We narrowed to 5,560 results for: SPI

1. pCS2-zfCSF3a

   Plasmid

   #168201

   Purpose

   Express zebrafish Csf3a, mRNA synthesis

   Depositor

   Petr Bartunek

   Article

   Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

   Insert

   Csf3a (csf3a Zebrafish)
   

   Use

   Vertebrate expression (zebrafish, chicken, xenopu…

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of A27

   Promoter

   Available since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCSN071

   Plasmid

   #166729

   Purpose

   Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae

   Depositor

   Rene Verwaal

   Article

   Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

   Insert

   dCas12a-Mxi1-NLS
   

   Use

   CRISPR

   Tags

   Mxi1 and SV40 NLS

   Expression

   Yeast

   Mutation

   E925A

   Promoter

   S. cerevisiae TEF1

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSN072

   Plasmid

   #166730

   Purpose

   Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae

   Depositor

   Rene Verwaal

   Article

   Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

   Insert

   dCas12a-NLS-Mxi1-NLS
   

   Use

   CRISPR

   Tags

   Mxi1 and SV40 NLS

   Expression

   Yeast

   Mutation

   E925A

   Promoter

   S. cerevisiae TEF1

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGBW-m4046774

   Plasmid

   #145574

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;FLAG_2

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pGBW-m4046846

   Plasmid

   #145575

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;HA

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pJS249

   Plasmid

   #134146

   Purpose

   Bacterial expression of human Ska1 microtubule binding domain (MTBD)

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   pHis-Precission-human Ska1 MTBD (SKA1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJS263

   Plasmid

   #134141

   Purpose

   Bacterial expression of C. elegans Ska1 microtubule binding domain (MTBD)

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   Ska1 MTBD-His (Y106G6H.15 Nematode)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIW087

   Plasmid

   #134128

   Purpose

   Bacterial expression of GFP-Ska1 and Ska2

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   His-GFP-human Ska1 ; human Ska2
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   K183A K184A

   Promoter

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIW084

   Plasmid

   #134130

   Purpose

   Bacterial expression of GFP-Ska1 and Ska2

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   His-GFP-human Ska1 ; human Ska2
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   R236A

   Promoter

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIW095

    Plasmid

    #134131

    Purpose

    Bacterial expression of GFP-Ska1 and Ska2

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-GFP-human Ska1 ; human Ska2
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    R155A

    Promoter

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIW090

    Plasmid

    #134129

    Purpose

    Bacterial expression of GFP-Ska1 and Ska2

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-GFP-human Ska1 ; human Ska2
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    K223A K226A

    Promoter

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIW093

    Plasmid

    #134137

    Purpose

    Retroviral expression of GFP-Ska1

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    CRISPR resistant, R155A

    Promoter

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIW082

    Plasmid

    #134136

    Purpose

    Retroviral expression of GFP-Ska1

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    CRISPR resistant, R236A

    Promoter

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIW088

    Plasmid

    #134135

    Purpose

    Retroviral expression of GFP-Ska1

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    CRISPR resistant, K223A K226A

    Promoter

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIW085

    Plasmid

    #134134

    Purpose

    Retroviral expression of GFP-Ska1

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    CRISPR resistant, K183A K184A

    Promoter

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Thy1.1-muSOX D219A

    Plasmid

    #131704

    Purpose

    encodes Thy1.1 followed by a P2A site and muSOX D219A catalytic mutant for enriching pure populations of transfected cells

    Depositor

    Britt Glaunsinger

    Article

    Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

    Insert

    Thy1.1-muSOX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Aspartic acid 219 mutated to an alanine

    Promoter

    CMV

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC0.007

    Plasmid

    #119671

    Purpose

    Level 0 part. Promoter, cpc560 promoter, extended spacer region, downstream TFB site region duplicated.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    Pcpc560-Dx2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    bp 89 T to C, bp 187 C to A, Duplicated downstrea…

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pC0.006

    Plasmid

    #119670

    Purpose

    Level 0 part. Promoter, cpc560 promoter, extended spacer region, upstream TFB site region duplicated.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    Pcpc560-Ux2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    bp 89 T to C, bp 187 C to A, Duplicated upstream …

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-puro-eGFP-HEC1-d80

    Plasmid

    #114025

    Purpose

    Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-d80-HEC1 (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Hec1 - 1 to 80 deleted

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5-hygro-eGFP-HEC1-d80

    Plasmid

    #114030

    Purpose

    Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-d80-HEC1 (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Hec1 - 1 to 80 deleted

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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