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MSCV-miRE_shBRD9_561-SV40-GFP

Plasmid

#75139

Purpose

Retroviral expression plasmid encoding shBRD9_561 (targeting human BRD)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBRD9_561

Use

Retroviral

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
pBS-KS-attB2-SA(2)-T2A-Gal4-Hsp70

Plasmid

#62898

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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pYFAC-CH4

Plasmid

#168980

Purpose

AMA1 based expression in filamentous fungi.pYFAC-pyroA carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA

Depositor

Yit Heng Chooi

Article

Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

Type

Empty backbone

Use

Synthetic Biology; Heterologous expression in asp…

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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PB-EF1-IRES-NEO deleted ITR

Plasmid

#67906

Purpose

PiggyBac transposon encoding Neomycin resistance with deleted inverted terminal repeat

Depositor

Alex Kentsis

Article

Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

Insert

NeoR (neoR Synthetic)

Expression

Mammalian

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pC-(loxP2-attB2-SA(0)-T2A-Gal4-Hsp70)

Plasmid

#62954

Purpose

Singlet donor (phase 0) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-p65AD-Hsp70

Plasmid

#62915

Purpose

Creating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-p65AD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pC-(loxP2-attB2-SA(1)-T2A-Gal4-Hsp70)

Plasmid

#62955

Purpose

Singlet donor (phase 1) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBLO 64.1

Plasmid

#123120

Purpose

E. coli expression vector for active deltaproteobacteria CasX

Depositor

Jennifer Doudna, Benjamin Oakes

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

CasX

Use

CRISPR

Promoter

NA

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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LB701: pMVP/PB/mCherry-DEST

Plasmid

#121871

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ mCherry selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-LexA::QFAD-Hsp70

Plasmid

#62948

Purpose

Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-LexA::QFAD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNAintron-CHIKV-Envelope protein

Plasmid

#215700

Purpose

Produces the Chikungunya virus envelope protein E3-E2-6K-E1

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV E3-E2-6K-E1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBS-KS-attB2-SA(1)-T2A-3X Gal80-Hsp70

Plasmid

#62952

Purpose

Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

3XGal80-FLAG/HA/EGFP-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-3X Gal80-Hsp70

Plasmid

#62953

Purpose

Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

3XGal80-FLAG/HA/EGFP-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
LC301: pMVP/PB/Zeo-DEST

Plasmid

#121877

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Zeo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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actb2-SecAnxaV-mKate2-acry-EGFP

Plasmid

#123628

Purpose

Ubiquitous zebrafish expression vector containing secreted human AnnexinV tagged to mKate2. Parton lab clone BDN.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

AnnexinA5

Tags

mKate2

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 S6K2 E388 D3E

Plasmid

#17731

Depositor

John Blenis

Article

Lee-Fruman et al Oncogene. 1999 Sep 9. 18(36):5108-14.

Insert

S6K2 (RPS6KB2 Human)

Tags

HA

Expression

Mammalian

Mutation

T388E, D3E(S410D, S417D, S423D): rapamycin-resist…

Promoter

CMV

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV Ef1a DIO GeNL_SS hGtACR1

Plasmid

#233511

Purpose

Luciferase opsin fusion protein (luminopsin) for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Ikefuama et al iScience. 2025 Feb 13;28(3):112022. doi:

Inserts

mNeonGreen
ISN-IE
hGtACR1

Use

AAV

Tags

Gaussia luciferase signal sequence

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pC-(lox2-attB2-SA-T2A-Gal4-Hsp70)3

Plasmid

#62957

Purpose

Triplet donor for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4 in three phases

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Ub[9]-mRFP[1]

Plasmid

#20249

Depositor

Nico Dantuma

Article

Salomons et al Mol Cell Biol. 2009 Apr;29(7):1774-85. d

Insert

ubiquitin C (UBC Human)

Tags

mRFP

Expression

Mammalian

Mutation

changed Stop codon 686 to Glycine

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-RiboL1-jGCaMP7c

Plasmid

#192619

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted (RiboL1) jGCaMP7c.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP7c

Use

AAV

Tags

6xHis

Expression

Mammalian

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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