We narrowed to 10,837 results for: ada

1. pLJM1 Flag Raptor HRas25

   Plasmid

   #26637

   Depositor

   David Sabatini

   Article

   Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

   Insert

   raptor-HRas25 (RPTOR Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pJW1354

   Plasmid

   #69490

   Purpose

   Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology arms

   Depositor

   Jordan Ward

   Article

   Zhang et al Development. 2015 Nov 9. pii: dev.129635

   Insert

   GSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
   

   Use

   CRISPR; Template to generate cassettes for -media…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. LRP6 deltaN-pCS2-VSVG

   Plasmid

   #27281

   Depositor

   Xi He

   Article

   Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

   Insert

   low density lipoprotein receptor-related protein 6 (LRP6 Human)
   

   Use

   Xenopus injection

   Tags

   VSVG

   Expression

   Mutation

   contains amino acid residues 1370–1613 (extracell…

   Promoter

   Available since

   Jan. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-cT3G-cERP2-MEIOC

   Plasmid

   #222562

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of MEIOC

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   MEIOC (C17orf104 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. px459 VRER

   Plasmid

   #101716

   Purpose

   sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E and T1337R)

   Promoter

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET22b-GFP-CPDSalI

   Plasmid

   #38257

   Depositor

   Aimee Shen, Matthew Bogyo

   Article

   Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

   Insert

   eGFP
   

   Use

   Tags

   6xHis and Vibrio cholerae MARTX toxin cysteine pr…

   Expression

   Bacterial

   Mutation

   cloned from pEGFPN3 (Clonetech)

   Promoter

   T7

   Available since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pBad-HaloGFP-Ca0.1(Na0.1, 143-144)

   Plasmid

   #198599

   Purpose

   Green fluorescent Ca2+ ion indicator for bacterial expression.

   Depositor

   Robert Campbell

   Article

   Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

   Insert

   HaloGFP-143-144
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Clathrin-LCa-ECFP

   Plasmid

   #21742

   Depositor

   Xiaowei Zhuang

   Article

   Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

   Insert

   Clathrin, light polypeptide (LCa) (Clta Mouse)
   

   Use

   Tags

   ECFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pRK-flag-USP19 1-1290

   Plasmid

   #78579

   Purpose

   Expression flag-USP19 1-1290 in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   flag-USP19 1-1290 (USP19 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   mutate 1291 residue to stop condon

   Promoter

   CMV

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pMK401 (Hygro-P2A-mAID-Nluc)

    Plasmid

    #121196

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID-Nluc
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. EK0501 SFFV-mCherry-SGc(MYC.s1)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #191185

    Purpose

    Expression of MYC sensor in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:MYC.s1:EGFP-3xMS2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. USP19 sgRNA2

    Plasmid

    #78586

    Purpose

    delete USP19 gene in human cell

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    USP19 sgRNA2 (USP19 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pspCas9-3'UTR-Tetra-com-vector

    Plasmid

    #132553

    Purpose

    Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

    Depositor

    Baisong Lu

    Article

    Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

    Inserts

    Cas9
    sgRNA scaffold with com replacing the Tetraloop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF6 rglut1 flag complete

    Plasmid

    #89571

    Purpose

    rglut1 flag complete

    Depositor

    Jeffrey Rathmell

    Article

    Wieman et al Biochem J. 2009 Mar 1;418(2):345-67. doi

    Insert

    rglut1 flag complete (Slc2a1 Rat)
    

    Use

    Tags

    Exo Facial

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAID1.2-CMV-CmCherry-mAID

    Plasmid

    #140602

    Purpose

    Expression of OsTIR1 and mAID-mCherry protein in DT40 cells under the control of CMV promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. NsvBa

    Plasmid

    #100015

    Purpose

    Electrophysiology in mammalian cells: Mammalian expression of a non-selective voltage gated ion channel and GFP

    Depositor

    David Clapham, Paul DeCaen

    Article

    DeCaen et al Elife. 2014 Nov 11;3. doi: 10.7554/eLife

    Insert

    NsvBa
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPB-cT3G-cERP2-DMC1

    Plasmid

    #222541

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of DMC1

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    DMC1 (DMC1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. MAC_N_SOX10

    Plasmid

    #167791

    Purpose

    Expresses SOX10 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    SOX10 (SOX10 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. MAC_N_NKX25

    Plasmid

    #167785

    Purpose

    Expresses NKX25 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    NKX25 (NKX2-5 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTK332

    Plasmid

    #59573

    Purpose

    SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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