We narrowed to 13,280 results for: cDNA

1. pcDNA5 CMV 3xFG eIF4G1

   Plasmid

   #158775

   Purpose

   pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform lacking the microexon.

   Depositor

   Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

   Insert

   eIF4G1
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

   Plasmid

   #182287

   Purpose

   Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
   PylT
   

   Use

   Tags

   nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   Y306A/Y384F (AF) double mutant of Methanosarcina …

   Promoter

   CMV and U6

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Delta(Pro-MP)ADAM10-HA

   Plasmid

   #65107

   Purpose

   Expression of HA tagged ADAM10 lacking prodomain and metalloprotease domain in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

   Insert

   Delta(Pro-MP)ADAM10 (ADAM10 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deleted AA 19-455

   Promoter

   CMV

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA4TO-24xGCN4_v4-BFP-24xPP7

   Plasmid

   #74929

   Purpose

   Translation reporter

   Depositor

   Marvin Tanenbaum

   Article

   Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

   Insert

   24xGCN4_v4-BFP-24xPP7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA GASTM-3-RVG-10-Lamp2b-HA

   Plasmid

   #71295

   Purpose

   Encodes (N to C): GASTM mutated glycosylation motif, 3 residue spacer, RVG peptide,10 residue spacer, Lamp2b (exosomal membrane protein), 3 residue spacer, HA tag.

   Depositor

   Joshua Leonard

   Article

   Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

   Insert

   Lamp2b (LAMP2 Human)
   

   Use

   Tags

   GASTM mutated glycosylation motif, HA, Lamp2 sig…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-H2B-AID-EYFP

   Plasmid

   #47329

   Purpose

   for plasmid integration using the Flp-In system. AID at C-terminal

   Depositor

   Don Cleveland

   Article

   Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

   Insert

   histone H2B (H2BC21 Human)
   

   Use

   Tags

   AID and EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Nuc-ExRai-AktAR2

   Plasmid

   #184049

   Purpose

   Nuclear-targeted ExRai-AktAR2.

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   Nuc-ExRai-AktAR2
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)

   Plasmid

   #117484

   Purpose

   Expresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Insert

   SpyCatcher002-6aa-sfCherry2(1-10)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-His:dCas9:nls:10P3

   Plasmid

   #135982

   Purpose

   Expresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cells

   Depositor

   Roman Jerala

   Article

   Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

   Insert

   dCas9 fused to 10 copies of the P3 coiled-coil
   

   Use

   CRISPR

   Tags

   His

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA5/FRT_CMV_NES-Kinprola_JNK-mEGFP

    Plasmid

    #233359

    Purpose

    CMV driven expression of the JNK activity recorder Kinprola_JNK in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Insert

    NES-Kinprola_JNK-mEGFP
    

    Use

    Tags

    NES and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA5/FRT_CMV_NES-Kinprola_AMPK-mEGFP

    Plasmid

    #233361

    Purpose

    CMV driven expression of the AMPK activity recorder Kinprola_AMPK in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Insert

    NES-Kinprola_AMPK-mEGFP
    

    Use

    Tags

    NES and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA5/FRT_CMV_NES-Kinprola_PKC-mEGFP

    Plasmid

    #233357

    Purpose

    CMV driven expression of the PKC activity recorder Kinprola_PKC in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Insert

    NES-Kinprola_PKC-mEGFP
    

    Use

    Tags

    NES and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-FRB-VMAc-CSarcin

    Plasmid

    #70223

    Purpose

    Expresses VMA intein with C-terminal portion of alpha-sarcin

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    alpha Sarcin (C-terminal portion)
    

    Use

    Tags

    FKBP-rapamycin binding (FRB) domain of the mammal…

    Expression

    Mammalian

    Mutation

    Changed Asp 28 to Cys, deleted amino acids 1-27

    Promoter

    CMV

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA 3.2/V5-DEST CDC42EP1 WT

    Plasmid

    #69819

    Purpose

    Expresses CDC42EP1-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    CDC42 effector protein (CDC42EP1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4TO-24xGCN4_v4-kif18b-24xPP7

    Plasmid

    #74928

    Purpose

    Translation reporter

    Depositor

    Marvin Tanenbaum

    Article

    Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

    Insert

    24xGCN4_v4-kif18b-24xPP7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-hLbCpf1(TYCV) (pY230)

    Plasmid

    #89355

    Purpose

    Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1, RR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    G532R/K595R

    Promoter

    CMV

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5/FRT/TO_b4g-HaloTag9

    Plasmid

    #175546

    Purpose

    Expression of HaloTag9 at the at the Gogli apparatus of the mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Beta4Gal-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 2xFLAG-2xSTREP E2F7

    Plasmid

    #236442

    Purpose

    transient overexpression of E2F7 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Tags

    FLAG-FLAG-STREP-STREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3 Flag-humanTRAF6 (1093)

    Plasmid

    #66929

    Purpose

    Mammalian Expression of TRAF6

    Depositor

    Michael Karin

    Insert

    TRAF6 (TRAF6 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1+-Tornado-split-nLuc

    Plasmid

    #212612

    Purpose

    For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.

    Depositor

    Samie Jaffrey

    Article

    Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

    Insert

    Tornado-CVB3-split-nLuc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    C373T mutation in CVB3

    Promoter

    CMV

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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