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pRSETB PKA RIalpha

Plasmid

#14922

Depositor

Susan Taylor

Article

Taylor et al Biochim Biophys Acta. 2005 Dec 30. 1754(

Insert

PKA R1alpha regulatory subunit (PRKAR1A Bovine)

Expression

Bacterial

Available Since

July 24, 2007

Availability

Academic Institutions and Nonprofits only

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pNIC-HIS10-ZBASIC-TEV-GG

Plasmid

#215797

Purpose

Cloning of target gene with N-terminal His10 Zbasic tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCasY1

Plasmid

#87687

Purpose

p15A plasmid expressing CasY1

Depositor

Jennifer Doudna

Article

Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

Insert

CasY1

Expression

Bacterial

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pTK204

Plasmid

#13534

Depositor

Wolf Frommer

Article

Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

Insert

FLIPW-CTYT

Tags

See map

Expression

Bacterial

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only

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pJC25NM

Plasmid

#1126

Depositor

Susan Lindquist

Article

Scheibel et al Nat Struct Biol 2001 Nov;8(11):958-62.

Insert

NM stop (SUP35 Budding Yeast)

Expression

Bacterial

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pMAK461

Plasmid

#140699

Purpose

NBαRb-IgG-Fc

Depositor

Takayuki Iwaki

Article

Iwaki et al Protein Expr Purif. 2020 Feb 13;170:1056

Insert

NBαRb-IgG-Fc

Expression

Bacterial

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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ovoD1 D1B2NR (18 kb)

Plasmid

#37280

Depositor

Norbert Perrimon

Article

Chou et al Development. 1993 Dec;119(4):1359-69.

Insert

ovoD1 genomic DNA fragment

Expression

Insect

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
pNIC- AVITAG-CTH10STII- -GG

Plasmid

#215805

Purpose

Cloning of target gene with C terminal non cleavable AVITAG and TEV cleavage of C terminal His10 and Twin Strep tag

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pRSETB PKA Cat

Plasmid

#14920

Depositor

Susan Taylor

Article

Taylor et al Biochim Biophys Acta. 2005 Dec 30. 1754(

Insert

PKA catalytic subunit alpha (Prkaca Mouse)

Expression

Bacterial

Available Since

July 24, 2007

Availability

Academic Institutions and Nonprofits only
P48

Plasmid

#212155

Purpose

Plasmid expressing 6x His tagged secondary alcohol dehydrogenase fused with superfolder green fluorescent protein at N terminus under constitutive promoter PgyrA control.

Depositor

Kang Zhou

Insert

superfolder green fluorescent protein, secondary alcohol dehydrogenase

Tags

6x His

Expression

Bacterial

Promoter

PgyrA

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pKM342

Plasmid

#71486

Purpose

Contains loxP-hyg-loxP cassette for recombineering; HygR, AmpR.

Depositor

Kenan Murphy

Article

Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

Insert

loxP-hyg-loxP cassette

Use

Source of loxp-hyg-loxp for pcr amplification.

Expression

Bacterial

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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CIT96u

Plasmid

#128018

Purpose

K77A

Depositor

Uwe Sauer

Article

Ewald et al PLoS One. 2011;6(12):e28245. doi: 10.137

Insert

CitA

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pTK164

Plasmid

#13533

Depositor

Wolf Frommer

Article

Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

Insert

FLIPW-CTY

Tags

ECFP and Venus

Expression

Bacterial

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only

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pMAK462

Plasmid

#140700

Purpose

NBαMs/Rt-IgG1-Fc

Depositor

Takayuki Iwaki

Article

Iwaki et al Protein Expr Purif. 2020 Feb 13;170:1056

Insert

NBαMs/Rt-IgG1-Fc

Expression

Bacterial

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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pET28Teb1

Plasmid

#28173

Depositor

Kathleen Collins

Article

Min et al Mol Cell. 2009 Nov 25. 36(4):609-19.

Insert

Teb1

Tags

6xhistidine

Expression

Bacterial

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
pNIC-HIS6-GST-TEV-GG

Plasmid

#215801

Purpose

Cloning of target gene with N terminal His6 GST tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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FLIParaF.Ec-250n

Plasmid

#18855

Depositor

Wolf Frommer

Article

Kaper et al Biotechnol Biofuels. 2008 Jun 3. 1(1):11

Insert

arabinose FRET sensor

Tags

CFP and Venus

Expression

Bacterial

Available Since

Nov. 12, 2008

Availability

Academic Institutions and Nonprofits only
pMAK463

Plasmid

#140701

Purpose

NBαMs-IgK

Depositor

Takayuki Iwaki

Article

Iwaki et al Protein Expr Purif. 2020 Feb 13;170:1056

Insert

NBαMs-IgK

Expression

Bacterial

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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pNIC-NHSTIIT-AVITAG-GG

Plasmid

#215802

Purpose

Cloning of target gene with N terminal His6 Twin Strep tag and TEV cleavage non cleavable N-terminal AVITAG

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDH22

Plasmid

#83798

Purpose

Useful for tagging yeast proteins with N-terminal YFP tag; G418 selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

YFP

Tags

YFP

Expression

Yeast

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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