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pLBH532_MBP-Cas14b1 expression

Plasmid

#112505

Purpose

Expresses 10xHis-MBP fused to Cas14b1 under t7 promoter

Depositor

Jennifer Doudna

Publication

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14b1

Use

CRISPR

Tags

MBP

Expression

Bacterial

Promoter

T7

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pRB1080

Plasmid

#71309

Purpose

Expresses VQR Cas9 variant with eef-1A.1 (eft-3) promoter

Depositor

Andrew Fire

Publication

Bell et al Genetics. 2015 Dec 17. pii: genetics.115

Insert

VQR Cas9 variant

Use

CRISPR

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

Plasmid

#216152

Purpose

Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

Depositor

Pietro Fratta

Publication

Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mScarlet with cryptic exon and C-terminal FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pENN226_CRISPRcharm

Plasmid

#220834

Purpose

CRISPRcharm expression in mammalian cells

Depositor

Jonathan Weissman

Publication

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CRISPRcharm

Use

CRISPR

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28a-mCherry-MED1-IDR

Plasmid

#194545

Purpose

Bacterial Expression of 6xHis mCherry-MED1-IDR Fusion Protein

Depositor

Denes Hnisz

Publication

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

MED1 (MED1 Human)

Tags

mCherry

Expression

Bacterial

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHR-UCOE-SFFV-Zim3-dCas9-BFP

Plasmid

#188776

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9-mTagBFP2

Use

Lentiviral

Tags

HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

Promoter

SFFV

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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ND4.2-Right DdCBE-G1397-C- T1413I-GFP

Plasmid

#179686

Purpose

Expression of base editor in mammalian cells and for cell sorting

Depositor

David Liu

Publication

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_P2A_GFP_ATP5B 3'UTR

Expression

Mammalian

Mutation

T1413I

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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sfGFP-Caveolin-C-10

Plasmid

#56366

Purpose

Localization: Membrane, Excitation: 485, Emission: 507

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Caveolin (CAV1 Human)

Tags

sfGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
tdTomato-CD9-10

Plasmid

#58076

Purpose

Localization: Tetraspanin/Membrane, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CD9 (CD9 Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pRB1080

Plasmid

#71309

Purpose

Expresses VQR Cas9 variant with eef-1A.1 (eft-3) promoter

Depositor

Andrew Fire

Publication

Bell et al Genetics. 2015 Dec 17. pii: genetics.115

Insert

VQR Cas9 variant

Use

CRISPR

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

Plasmid

#216152

Purpose

Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

Depositor

Pietro Fratta

Publication

Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mScarlet with cryptic exon and C-terminal FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pENN226_CRISPRcharm

Plasmid

#220834

Purpose

CRISPRcharm expression in mammalian cells

Depositor

Jonathan Weissman

Publication

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CRISPRcharm

Use

CRISPR

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-28a-mCherry-MED1-IDR

Plasmid

#194545

Purpose

Bacterial Expression of 6xHis mCherry-MED1-IDR Fusion Protein

Depositor

Denes Hnisz

Publication

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

MED1 (MED1 Human)

Tags

mCherry

Expression

Bacterial

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
pHR-UCOE-SFFV-Zim3-dCas9-BFP

Plasmid

#188776

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9-mTagBFP2

Use

Lentiviral

Tags

HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

Promoter

SFFV

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
ND4.2-Right DdCBE-G1397-C- T1413I-GFP

Plasmid

#179686

Purpose

Expression of base editor in mammalian cells and for cell sorting

Depositor

David Liu

Publication

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_P2A_GFP_ATP5B 3'UTR

Expression

Mammalian

Mutation

T1413I

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
sfGFP-Caveolin-C-10

Plasmid

#56366

Purpose

Localization: Membrane, Excitation: 485, Emission: 507

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Caveolin (CAV1 Human)

Tags

sfGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
tdTomato-CD9-10

Plasmid

#58076

Purpose

Localization: Tetraspanin/Membrane, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CD9 (CD9 Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAA3.stelN

Plasmid

#112011

Purpose

Expresses TelN enzyme

Depositor

Stephen Harrison

Publication

Jenni et al Science. 2018 May 4;360(6388):552-558. d

Insert

TelN

Tags

strep and his

Expression

Bacterial

Promoter

T7

Available since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBBR1k-con-GFPuv

Plasmid

#106386

Purpose

Constitutive trc promoter expressing gfpuv on BBR1 origin

Depositor

Brian Pfleger

Publication

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

trc

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAA3.stelN

Plasmid

#112011

Purpose

Expresses TelN enzyme

Depositor

Stephen Harrison

Publication

Jenni et al Science. 2018 May 4;360(6388):552-558. d

Insert

TelN

Tags

strep and his

Expression

Bacterial

Promoter

T7

Available since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge