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Showing: 4041 - 4060 of 7257 results

  4. pH4-DEST

    Plasmid
    #90099
    Purpose
    Gateway destination vector featuring H4 promoter in front of AttR1/2 sites on episomal vector maintained in the diatom Phaeodactylum tricornutum.
    Depositor
    Philip Weyman
    Article
    Bielinski et al PeerJ Preprints. 2017 5:e2907v1
    Type
    Empty backbone
    Use
    Gateway destination vector for protein expression…
    Tags
    Expression
    Mutation
    Promoter
    H4
    Available since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    90099-plasmid-map-sequence-id-179765

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  5. pOpen-T4 g32

    Plasmid
    #165560
    Purpose
    A single-stranded DNA binding protein required for bacteriophage T4 replication and repair; Used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structure, to increase yield and processivity of reverse transcriptase during RT-PCR, and to increase yield and specificity of PCR products from soil samples.
    Depositor
    Jennifer Molloy, Drew Endy, FreeGenes Project
    Insert
    T4 gene 32
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165560-plasmid-map-sequence-id-339953

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  8. pFcpB-DEST

    Plasmid
    #90098
    Purpose
    Gateway destination vector featuring FcpB promoter in front of AttR1/2 sites on episomal vector maintained in the diatom Phaeodactylum tricornutum.
    Depositor
    Philip Weyman
    Article
    Bielinski et al PeerJ Preprints. 2017 5:e2907v1
    Type
    Empty backbone
    Use
    Destination vector for gateway expression
    Tags
    Expression
    Mutation
    Promoter
    FcpB
    Available since
    May 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    90098-plasmid-map-sequence-id-179764

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  11. pCMV6-XL4 FLAG-LRRC3-Fc

    Plasmid
    #115766
    Purpose
    Expression of the extracellular domain of LRRC3 with N-terminal FLAG tag and C-terminal hIgG tag.
    Depositor
    Davide Comoletti
    Insert
    LRRC3 (LRRC3 Human)
    Use
    Tags
    FLAG and hIgG/Fc
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    115766-plasmid-map-sequence-id-223835

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  12. pCMV6-XL4 FLAG-CBLN3-Fc

    Plasmid
    #115768
    Purpose
    Expression of the extracellular domain of CBLN3 with N-terminal FLAG tag and C-terminal hIgG tag.
    Depositor
    Davide Comoletti
    Insert
    CBLN3 (CBLN3 Human)
    Use
    Tags
    FLAG and hIgG/Fc
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    115768-plasmid-map-sequence-id-223837

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  13. pT7CFE1_EVD68_VP12A_C107R

    Plasmid
    #212982
    Purpose
    Encodes EV-D68 MO/14/18947 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 VP12A C107R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    changed cysteine 107 from 2A to Arginine
    Promoter
    T7
    Available since
    Feb. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212982-plasmid-map-sequence-id-422577

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  14. pT7CFE1_EVD68_3ABC_C147R

    Plasmid
    #212978
    Purpose
    Encodes EV-D68 MO/14/18947 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 3ABC C147R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    changed Cysteine 147 in 3C to Arginine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212978-plasmid-map-sequence-id-420358

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  15. pT7CFE1_EVD68_3ABCD_C147R

    Plasmid
    #212980
    Purpose
    Encodes EV-D68 MO/14/18947 3ABCD with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 3ABCD C147R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    changed cysteine 147 from 3C to arginine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212980-plasmid-map-sequence-id-420353

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  16. pT7CFE1_EVD68_VP12A_N84T

    Plasmid
    #212983
    Purpose
    Encodes EV-D68 MO/14/18947 VP12A with telaprevir resistance mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 VP12A N84T
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    changed Asparagine 84 from 2A to Threonine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212983-plasmid-map-sequence-id-420352

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  17. pAc5.1B-V5-SBP-DmPop2a_K

    Plasmid
    #146723
    Purpose
    Insect Expression of DmPop2a
    Depositor
    Elisa Izaurralde
    Insert
    DmPop2a (Pop2 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    ORF extracted from CG5684-PA; NM_140281.2, with o…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146723-plasmid-map-sequence-id-289169

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  18. pJM102: pHps2 RFP on pCM66T

    Plasmid
    #74741
    Purpose
    pCM66T with promoter pHps2 driving RFP expression (maxRFP = BBa_E1010)
    Depositor
    Mary Lidstrom
    Insert
    RFP (BioBrick part number BBa_E1010)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pHps2 (extracted from Methylovorus glucosetrophus…
    Available since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74741-plasmid-map-sequence-id-141458

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Showing: 4041 - 4060 of 7257 results