We narrowed to 5,172 results for: PEP

1. MET7A-CD4d3+4-bio

   Plasmid

   #73127

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding MET7A with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   MET7A (METTL7A Human)
   

   Use

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV BARK D110A p2A mCherry

   Plasmid

   #117690

   Purpose

   CMV-iBARK(D110A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencing

   Depositor

   Baljit Khakh

   Article

   Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

   Insert

   BARKrgs D110A peptide
   

   Use

   Tags

   HA / FLAG

   Expression

   Mammalian

   Mutation

   D110A

   Promoter

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 3xFlag IFIT3

   Plasmid

   #53553

   Purpose

   mammalian expression of IFIT3

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT3 (IFIT3 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pME-H2B-mCerulean3-P2A-mKOFP2-CAAX

   Plasmid

   #75154

   Purpose

   Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   H2B-mCerulean3-P2A-mKOFP2-CAAX
   

   Use

   Middle entry vector for multisite gateway three f…

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. SSTR3-DuET

   Plasmid

   #213378

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   SSTR3 (SSTR3 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBT2-4xUAS:P2A-mCerulean3

   Plasmid

   #127592

   Purpose

   This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with mCerulean3 through P2A self-cleaving peptides.

   Depositor

   Longhou Fang

   Article

   Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

   Type

   Empty backbone

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. HMBP-3C-STAR.66-285

   Plasmid

   #100094

   Purpose

   expresses MBP-tagged STARD1 protein domain (residues 66-285)

   Depositor

   Nikolai Sluchanko

   Article

   Sluchanko et al Protein Expr Purif. 2016 Mar;119:27-35.

   Insert

   STAR protein (STAR Human)
   

   Use

   Tags

   His-tagged MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYSD313

   Plasmid

   #212931

   Purpose

   Encodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   Sed1 (SED1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Activin A (S2-2)

   Plasmid

   #184621

   Purpose

   for transfection into S2 cells and expression of Activin A protein

   Depositor

   Timothy Springer

   Article

   Le et al J Mol Biol. 2022 Mar 15;434(5):167439. d

   Insert

   INHBA (INHBA Human)
   

   Use

   Tags

   8x His - streptavidin binding peptide

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4-DU422gp140-TM

    Plasmid

    #123251

    Purpose

    Mammalian expression plasmid for soluble gp140 from the DU422 HIV-1 isolate with a transmembrane tether

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (DU422) Env
    

    Use

    Tags

    CD5 leader peptide and Linker (with 6his tag) and…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNUT N6His hTFNG

    Plasmid

    #67240

    Purpose

    Expresses N-His tagged nonglycosylated human serum transferrin in mammalian cells

    Depositor

    Anne Mason, Ross MacGillivray

    Article

    Mason et al Biochemistry. 2002 Jul 30;41(30):9448-54

    Insert

    human serum transferrin (TF Human)
    

    Use

    Tags

    N-terminal signal peptide, 4 aa link, 6 His, Fact…

    Expression

    Mammalian

    Mutation

    Asn413 Asp, Asn611Asp

    Promoter

    SV40

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. NTSR1-DuET

    Plasmid

    #213356

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    NTSR1 (NTSR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EWB-DIO-myrTagRFP-T-P2A-post-eGRASP

    Plasmid

    #111581

    Purpose

    An AAV vector that expresses double floxed myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Science. 2018 Apr 27;360(6387):430-435.

    Insert

    myrTagRFP-T-P2A-post-eGRASP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. SSTR2-DuET

    Plasmid

    #213377

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    SSTR2 (SSTR2 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ173

    Plasmid

    #99907

    Purpose

    Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Insert

    pco-dCas9-VP64-T2A-MS2-VP64
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. SWEET11:SWEET11-2A-GFP-GUS

    Plasmid

    #172484

    Purpose

    Translational fusion of SWEET11 with a P2A self cleaving peptide sequence

    Depositor

    Wolf Frommer

    Article

    Kim et al Plant Cell. 2021 May 5;33(3):511-530. do

    Insert

    SWEET11 (AT3G48740 Mustard Weed)
    

    Use

    Tags

    GFP, GUS

    Expression

    Plant

    Mutation

    Promoter

    SWEET11

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-RBP-LOV2

    Plasmid

    #185792

    Purpose

    Encodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linker

    Depositor

    Lizhong He

    Article

    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

    Insert

    RBP-LOV2
    

    Use

    Tags

    10xHis, TEV protease cleavage site after His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCEP4-myc-HLA-A3

    Plasmid

    #135505

    Purpose

    Mammalian expression of myc-tagged HLA-A*03:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*03:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR P2R-P3-SV40 polyA signal

    Plasmid

    #48627

    Purpose

    Contributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.

    Depositor

    Tomas Santalucia, Anna Planas

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    SV40 early polyadenylation signal cassette
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-p58

    Plasmid

    #166942

    Purpose

    mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with GFP

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70.

    Insert

    p58 (Lman1 Rat)
    

    Use

    Tags

    GFP and chicken lysozyme signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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