We narrowed to 31,681 results for: PLE
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Plasmid#220470PurposeDual luciferase reporter for RNAi, with target site within a tunable ORF (version B)DepositorInsertSUMO_Eu1
Tags3xHAExpressionMammalianPromoterMinimal CMVAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSliceRep_A_miR-7
Plasmid#220464PurposeDual luciferase reporter for RNAi, with target site within a tunable ORF (version A)DepositorInsertSuperfolder GFP
Tags3xFLAGExpressionMammalianPromoterMinimal CMVAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSliceRep_A_miR-7.M4
Plasmid#220465PurposeDual luciferase reporter for RNAi, with target site within a tunable ORF (version A)DepositorInsertSuperfolder GFP
Tags3xFLAGExpressionMammalianPromoterMinimal CMVAvailable SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
nTALE+CD_for TUB_8N_1397NC
Plasmid#193383PurposenTALECD (1397NC) expression vectorDepositorInsertFor multiplex C-to-T base editing to introduce amino acid mutations in the coding sequence of AtTUBs.
ExpressionPlantAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfuNca-MCS
Plasmid#190820PurposeMultiple cloning site between Nitzschia captiva promoter and termination regionsDepositorInsertsMultiple cloning site with SpeI and PstI
Bleomycin resistance gene
ExpressionBacterialAvailable SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pLux LuxI
Plasmid#193627PurposeInducible expression of LuxI by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL). LuxI is codon optimized for E. coli.DepositorInsertLuxI
Tags6xHisExpressionBacterialPromoterLux promoterAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
Atg20-24 pST39
Plasmid#200439PurposeHis6 tagged Atg20 and untagged Atg24 from S. cerevisiae for co-expression in E. coli.DepositorInsertsAtg20
Atg24
Tags6xHis-TEVExpressionBacterialAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSumo-His6-SUMO-AtTPR2(1-209)
Plasmid#177860PurposeBacterial Expression of TPR2DepositorAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1-tTA/Zeo
Plasmid#171685Purposeconstitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (zeocin)DepositorInsertZeocin
ExpressionMammalianPromoterRPBSA promoterAvailable SinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCS-TCCC
Plasmid#165774PurposeMultiple cloning site part for enhancer cloning, requires minimal promoter elementDepositorInsertMCS-TCCC
ExpressionInsectAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-wzzBST
Plasmid#172968PurposepWSK29 with Salmonella typhimurium wzzBST geneDepositorInsertwzzB gene from Salmonella enterica serovar Typhimurium
ExpressionBacterialMutationK198E- please see depositor commentsAvailable SinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-fepEST
Plasmid#172969PurposepWSK29 with Salmonella typhimurium fepEST geneDepositorInsertfepE gene from Salmonella enterica serovar Typhimurium
ExpressionBacterialMutationG160S- please see depositor commentsAvailable SinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCL12-decoder
Plasmid#159447PurposeInsert: PlexAtet-citrine-tCyc1. The fluorescent protein Citrine is inducible by LexA-ER-B112 upon addition of estradiol, and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.DepositorInsertPlexAtet-Citrine-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPlexAtetAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8637 PB-PGK-C intein-2XNLS-miniVPR-mCherry-pA
Plasmid#140224PurposeConstitutive PGK driven expression of intein with miniVPR for 3-way split activatorDepositorInsertC-intein with miniVPR
TagsmCherryExpressionMammalianPromoterPGKAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMtnA eGFP MALAT1
Plasmid#132643PurposeExpresses an eGFP mRNA from the Drosophila Metallothionein A promoter that ends in the MALAT1 triple helixDepositorInserteGFP
ExpressionInsectPromoterMtnAAvailable SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL1211
Plasmid#129521PurposepDEF-SpCas9::GFP, For deletion of pyalba4, PyGAPDH promoter, 2nd GenerationDepositorInsertSpCas9
UsePlasmodium, rodent-infectious speciesTags3XFlag-NLSAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAW218
Plasmid#113240PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flipDepositorInsertsCas1
Cas2
Leader-repeat
ExpressionBacterialMutationAAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…Available SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-pcr1P-natMX6-pcr2P
Plasmid#80620Purposeto delete part of the mat1-P locus and fix the mating type in natural S. pombe isolatesDepositorInsertsPCR1 from mat1-P
PCR2 from mat1-P
ExpressionYeastAvailable SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-pcr1M-natMX6-pcr2M
Plasmid#80621Purposeto delete part of the mat1-M locus and fix the mating type in natural S. pombe isolatesDepositorInsertsPCR1 from mat1-M
PCR2 from mat1-M
ExpressionYeastAvailable SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-2
Plasmid#61737PurposeStreptomyces expression of codon-optimized Cas9 and custom gRNADepositorInsertssSpCas9
gRNA cassette
UseCRISPRExpressionBacterialMutationBbsI-flanked lacZ cassette inserted in place of s…Promotergapdhp(EL) and rpsL(XC)-BbsIAvailable SinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only