We narrowed to 5,560 results for: SPI

1. T/Brachyury-eGFP Rex Neo

   Plasmid

   #21222

   Depositor

   Mark Mercola

   Article

   Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

   Insert

   Brachyury promoter
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   July 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7-7 asyn S129E

   Plasmid

   #36050

   Depositor

   Hilal Lashuel

   Article

   Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

   Insert

   alpha-synuclein S129E (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S129E

   Promoter

   T7

   Available since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-hACE2-LgBiT

   Plasmid

   #215837

   Purpose

   Expresses hACE2 receptor with LgBiT tag in mammalian cells

   Depositor

   Thomas Gallagher

   Article

   Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

   Insert

   hACE2-LgBiT (ACE2 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

   Plasmid

   #171588

   Purpose

   Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter

   Depositor

   Kenneth Matreyek

   Article

   Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

   Inserts

   Bxb1 integrase
   iCasp9
   BFP
   rtTA3G
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-mEGFP-CaMKIIa

   Plasmid

   #127389

   Purpose

   Fluorescent reporter for Ca2+/calmodulin-dependent protein kinase II alpha

   Depositor

   Ryohei Yasuda

   Article

   Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

   Insert

   calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJRH-1364 LV EF1a-CD4 IRES-EGFP

   Plasmid

   #201920

   Purpose

   2nd generation lentiviral transfer plasmid for engineering cells to express human CD4

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   CD4 (CD4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TUBB5-Halo

   Plasmid

   #64691

   Purpose

   Expresses beta-tubulin labeled with Halo-tag

   Depositor

   Yasushi Okada

   Article

   Uno et al Nat Chem. 2014 Aug;6(8):681-9. doi: 10.1

   Insert

   TUBB
   

   Use

   Tags

   Halo-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. EF1a-LCB1_v1.3-PDGFR-T2A-mCherry (pZYW048)

   Plasmid

   #194231

   Purpose

   Lentiviral expression vector expressing LCB1 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   EF1a-LCB1-PDGFR TMD-T2A-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTE4398

   Plasmid

   #74042

   Purpose

   Expresses human codon-optimized LbCpf1 and Lb crRNA.

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   Lb crRNA
   LbCpf1
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and human U6

   Available since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. mEGFP-HRas G12V

    Plasmid

    #18666

    Depositor

    Karel Svoboda

    Article

    Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

    Insert

    H-Ras (HRAS Human)
    

    Use

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Mutation

    G12V mutation in HRas

    Promoter

    Available since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. Stargazin-GFP-LOVpep

    Plasmid

    #80406

    Purpose

    Membrane localized GFP-tagged LOVpep. Expresses in mammalian cells

    Depositor

    Michael Glotzer

    Article

    Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

    Insert

    Stargazin-GFP-LOVpep (CACNG2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)

    Plasmid

    #160611

    Purpose

    Transcriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    P35s:LbCas12a:T35s
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAT003

    Plasmid

    #171628

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-TRAC
    mNeon
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and EF1-a

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-IRES-puro-NLS-dLbaCas13a-EGFP-NLS-3xFlag

    Plasmid

    #132408

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dLbaCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    R600A; H605A; R1243A; H1248A

    Promoter

    Available since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. 6691 Bicistronic_ires_puro

    Plasmid

    #64335

    Purpose

    This a retroviral expression plasmid with a minimal polylinker followed by IRES and puro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. BDNF-SEP

    Plasmid

    #83955

    Purpose

    BDNF with pH sensitive fluorophore for visualization of release

    Depositor

    Ryohei Yasuda

    Article

    Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

    Insert

    Brain derived neurotrophic factor (Bdnf Mouse)
    

    Use

    Tags

    SEP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-C1-MKLP1

    Plasmid

    #70145

    Purpose

    mammalian expression of MKLP1 with an eGFP fusion

    Depositor

    Masanori Mishima

    Article

    Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

    Insert

    MKLP1 (KIF23 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ141-ZmUbi-RZ-Lb

    Plasmid

    #86197

    Purpose

    LbCpf1 Gateway gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    gRNA cloning site
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Maize ubiquitin 1

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLGB36

    Plasmid

    #135621

    Purpose

    Suicide vector for allelic replacement in Bacteroides species including B. fragilis strain 638R, erythromycin selection and aTC-inducible ss-Bfe3 counterselection

    Depositor

    Laurie Comstock

    Article

    Ito et al mBio. 2020 Feb 4;11(1). pii: mBio.03238-

    Insert

    bfe3
    

    Use

    Bacteroides suicide vector with inducible counter…

    Tags

    signal sequence for periplasmic targeting

    Expression

    Mutation

    Promoter

    Bacteroides promoter regualted by TetR transcript…

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. ePop

    Plasmid

    #50953

    Purpose

    Expresses E lysis protein that causes in subpopulation death when threshold cell density is reached, resulting in oscillations

    Depositor

    Lingchong You

    Article

    Marguet et al PLoS One. 2010 Jul 30;5(7):e11909. doi:

    Inserts

    E lysis protein
    LuxI
    LuxR
    

    Use

    Tags

    Expression

    Mutation

    Early stop codon introduced in mutated luxR resul…

    Promoter

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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