We narrowed to 4,111 results for: plasmid lenti crispr

1. pDECKO_GFP

   Plasmid

   #141210

   Purpose

   empty GFP vector for cloning sgRNA or pgRNAs

   Depositor

   Rory Johnson

   Article

   Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFUGW-shRIIα-RIIαWT-IRES-EGFP

   Plasmid

   #183456

   Purpose

   Replacement of endogenous rat PKA-RIIα subunits with wild-type RIIα

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   sgRNA and Prkar2a (Prkar2a Rat)
   

   Use

   Lentiviral

   Mutation

   shRNA-resistant mutations: C135>G, G138>A, …

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFUGW-shRIIα-RIIαS97A-IRES-EGFP

   Plasmid

   #183457

   Purpose

   Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variant

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   sgRNA and Prkar2a (Prkar2a Rat)
   

   Use

   Lentiviral

   Mutation

   shRNA-resistant mutations: C135>G, G138>A, …

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFUGW-shRIIα-RIIαS97E-IRES-EGFP

   Plasmid

   #183458

   Purpose

   Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variant

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   sgRNA and Prkar2a (Prkar2a Rat)
   

   Use

   Lentiviral

   Mutation

   shRNA-resistant mutations: C135>G, G138>A, …

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX301-ZIM3-KRAB-PYL1

   Plasmid

   #154761

   Purpose

   Expresses ZIM3 KRAB domain fused to PYL1

   Depositor

   Mikko Taipale

   Article

   Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

   Insert

   ZIM3 (ZIM3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Includes ZIM3 aa 1-100

   Promoter

   CMV

   Available Since

   Oct. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pKLV2.2-h7SKgRNA5(ARID1A(44))-hU6gRNA5(AAVS1)-PGKpuroBFP-W

   Plasmid

   #200506

   Purpose

   Lentiviral vector expressing gRNA targeting human ARID1A and AAVS1

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   ARID1A(44), AAVS1 (ARID1A, AAVS1 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(AAVS1)-PGKpuroBFP-W

   Plasmid

   #200503

   Purpose

   Lentiviral vector expressing gRNA targeting human SMARCA4 and AAVS1

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   SMARCA4(43), AAVS1 (SMARCA4, AAVS1 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tet-pLKO-puro-shTAL1

   Plasmid

   #210640

   Purpose

   Knockdown of TAL1 endogenous (3'UTR)

   Depositor

   Maayan Salton

   Article

   Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

   Insert

   TAL1 3' UTR gRNA (TAL1 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLCAR-CD19-28z

   Plasmid

   #135991

   Purpose

   Modular CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Scott McComb

   Article

   Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

   Insert

   FMC63-28-3z-P2A-EGFP (CD28, CD247 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a-short

   Available Since

   June 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSLCAR-CD19-CD3z

    Plasmid

    #135993

    Purpose

    Modular CD3z alone CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Scott McComb

    Article

    Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

    Insert

    FMC63-3z-P2A-EGFP (CD247, CD28 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a-short

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLCAR-CD19-BBz

    Plasmid

    #135992

    Purpose

    Modular 41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Scott McComb

    Article

    Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

    Insert

    FMC63-41BB-3z-P2A-EGFP (CD247, CD28, TNFRSF9 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a-short

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LV2btCRISPRko.v1

    Pooled Library

    #213927

    Purpose

    Uses lentivirus to deliver the library into hard-to-transduce cells

    Depositor

    Wenfang Spring Tan

    Article

    Tan et al PLoS Pathog. 2023 Dec 6;19(12):e1011822.

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

13. MYC-CRISPR

    Pooled Library

    #173195

    Purpose

    This pooled lentiviral CRISPR knockout library targets E-boxes genome-wide and can be applied for identification of essential MYC binding sites in a wide range of human cells.

    Depositor

    Agnieszka Dzikiewicz-Krawczyk

    Article

    Kazimierska et al Mol Oncol. 2023 Nov;17(11):2295-2313. do

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

14. Human CRISPR Metabolic Gene Knockout Library

    Pooled Library

    #110066

    Purpose

    Encodes gRNAs for targeting metabolic genes in CRISPR screens.

    Depositor

    David Sabatini

    Article

    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

    Use

    Lentiviral

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

15. Human ubiquitination-related proteins CRISPR KO library

    Pooled Library

    #174592

    Purpose

    CRISPR Cas9 KO library targets 660 ubiquitination-related proteins, including all major E1, E2, E3 and deubiquitinating enzymes, as well as other ubiquitination-related proteins.

    Depositor

    Juan Bonifacino

    Article

    Jia et al Elife. 2019 Nov 6;8. pii: 50034. doi: 10

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

16. Human Metabolic Gene CRISPRa sgRNA library

    Pooled Library

    #187080

    Purpose

    Lentiviral CRISPRa library targeting human metabolic genes for activation of gene expression

    Depositor

    Kivanc Birsoy

    Article

    Unlu et al Mol Cell. 2022 Aug 4;82(15):2832-2843.e7

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    Lentiviral

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

17. Mouse Metabolic Gene CRISPRa sgRNA library

    Pooled Library

    #187081

    Purpose

    Lentiviral CRISPRa library targeting mouse metabolic genes for activation of gene expression

    Depositor

    Kivanc Birsoy

    Article

    Unlu et al Mol Cell. 2022 Aug 4;82(15):2832-2843.e7

    Expression

    Mammalian

    Species

    Mus musculus

    Use

    Lentiviral

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

18. PBbtCRISPRko.v1

    Pooled Library

    #213928

    Purpose

    Uses the PiggyBac transposon system to deliver the library into hard to transduce cells

    Depositor

    Wenfang Spring Tan

    Article

    Tan et al PLoS Pathog. 2023 Dec 6;19(12):e1011822.

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

19. Human CRISPR Poison Exon Knockout Library

    Pooled Library

    #138084

    Purpose

    The human pgPoison library induces skipping of “poison” cassette exons and corresponding upstream constitutive exons by targeting 3' splice sites with paired gRNAs (pgRNAs).

    Depositor

    Robert Bradley

    Article

    Thomas et al Nat Genet. 2020 Jan;52(1):84-94. doi: 10

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

20. Mouse SLC KO Library

    Pooled Library

    #141436

    Purpose

    Expresses gRNAs for knockout of the mouse Solute Carrier family

    Depositor

    Giulio Superti-Furga

    Expression

    Mammalian

    Species

    Mus musculus

    Use

    CRISPR and Lentiviral

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only