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pBS-I-Sce1-slc6a3[11kb]:egfp

Plasmid

#53240

Purpose

allow visualization of pretectal dopaminergic neurons in vivo from day 3 post-fertilization and allow expression of transgenes in these neurons through adulthood

Depositor

Edward Burton

Article

Bai et al Neuroscience. 2009 Dec 15;164(3):1138-51

Insert

DAT-GFP

Use

Cloning

Tags

GFP

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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GADD45 P-3m-luc

Plasmid

#8358

Depositor

Michael Greenberg

Article

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

GADD45 promoter P-3m (GADD45A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

P-3m

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
L_NES 67kDa

Plasmid

#201357

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Adeno_NES-EGFP-6PrA

Expression

Mammalian

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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M_NLS 67kDa

Plasmid

#201351

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A5-EGFP-6PrA

Expression

Mammalian

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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H_NES 67kDa

Plasmid

#201350

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

HIV_NES-EGFP-6PrA

Expression

Mammalian

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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M_NES 67kDa

Plasmid

#201356

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

MAPK_NES-EGFP-6PrA

Expression

Mammalian

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_Notch1 del-NRR

Plasmid

#188353

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-del-NRR-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_Notch1-NRRdeletion-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBTK401

Plasmid

#110597

Purpose

BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

mRFP dropout (RSF1010 origin)

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pHES873

Plasmid

#87945

Purpose

Expresses estradiol-inducible synthetic transcription factor in yeast under control of GAL1 promoter. It activates transcription of promoters containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pGAL1- Zif268 DBD - hPR LBD - MSN2 AD (PGR Human, Budding Yeast)

Expression

Yeast

Promoter

GAL1

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pEF HA-TSAdwt

Plasmid

#84517

Purpose

Mammalian expression of TSAd

Depositor

Anne Spurkland

Article

Granum et al BMC Immunol. 2006 Jul 13;7:15.

Insert

TSAd (SH2D2A Human)

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-DRD1-NNES-eLOV-TEVcs-Flag-Gal4-V5

Plasmid

#125227

Purpose

Expresses SPARK DRD1 transmembrane component in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

DRD1-NNES-eLOV-TEVcs-Flag-Gal4-V5

Use

AAV

Tags

Flag, V5

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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Pmyo3::tag:sl1(G16C)

Plasmid

#79003

Purpose

Expression of exogenous SL1 in C. elegans muscle

Depositor

Xiao Liu, Ge Zhan

Article

Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw

Insert

sls-1.2 (sls-1.2 Nematode)

Tags

illumina-adapter

Expression

Worm

Mutation

G16C

Promoter

myo-3

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBTK550d

Plasmid

#110617

Purpose

BTK assembled broad-host-range plasmid encoding inducible GFP driven by T7 polymerase

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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L1-1147

Plasmid

#13267

Depositor

Vance Lemmon

Article

Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

Insert

L1CAM (L1CAM Human)

Expression

Bacterial

Mutation

Truncated in the juxtamembrane region

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pEPQD1CB0109

Plasmid

#177042

Purpose

MoClo Level 1, position 2, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies driven by 35S promoter; contains a chloroplast transit peptide

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geranyl pyrophosphate synthase (PaGPPS1) from Picea abies

Use

Synthetic Biology

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-IgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc

Plasmid

#125229

Purpose

Expresses transmembrane intracellular NanoLuc in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

IgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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Pmyo-3::tag:SL1(G20A)

Plasmid

#81114

Purpose

Expression of exogenous SL1(G20A) in C. elegans muscle

Depositor

Xiao Liu

Article

Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw

Insert

sls-1.2 (sls-1.2 Nematode)

Tags

illumina-adapter

Expression

Worm

Mutation

G20A

Promoter

promoter of myo-3

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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RP78 pUAST Orco mRNA

Plasmid

#53431

Purpose

Expresses the full-length Drosophila melanogaster Orco cDNA under Gal4/UAS control

Depositor

Leslie Vosshall

Article

Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

Insert

Orco (Orco )

Expression

Insect

Available Since

Aug. 12, 2014

Availability

Academic Institutions and Nonprofits only
pMS7: pCascade(AvCAST)_entry

Plasmid

#168140

Purpose

Inducible expression of AvCAST Cascade proteins. Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-RELΔ424-490

Plasmid

#27267

Depositor

Thomas Gilmore

Article

Starczynowski et al Oncogene. 2007 Apr 26. 26(19):2685-94.

Insert

REL (REL Human)

Expression

Mammalian

Mutation

Deletion of transactivation domain I (aa 425-490)

Available Since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only