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NUMB4-eGFP-M107K

Plasmid

#37805

Depositor

Rolf Bjerkvig

Publication

Euskirchen et al Exp Cell Res. 2011 Dec 10;317(20):2864-7

Insert

NUMB isoform 4 (NUMB Human)

Use

Lentiviral

Tags

eGFP

Promoter

pCMV

Available since

March 13, 2013

Availability

Academic Institutions and Nonprofits only

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pBrain-GFP-TACC3KDP-shTACC3

Plasmid

#59356

Purpose

Knocks down endogenous TACC3 and re-expresses knockdown-proof (RNAi-resistant) GFP-tagged human TACC3.

Depositor

Stephen Royle

Publication

Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Use

RNAi

Tags

GFP

Expression

Mammalian

Mutation

Silent mutations in amino acids 30-33 to confer s…

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pETMutS

Plasmid

#13245

Depositor

Peggy Hsieh

Publication

Biswas et al J Biol Chem 1996 Mar 1;271(9):5040-8.

Insert

MutS

Expression

Bacterial

Mutation

None

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pDONR223-PIP5K1B

Plasmid

#23848

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIP5K1B (PIP5K1B Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

Plasmid

#158651

Purpose

making transgenics expressing a UAS driven YFP and NTR 2.0

Depositor

Jeff Mumm

Publication

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:BFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Mutation

F70A/F108Y

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223-CSNK2A1

Plasmid

#23678

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CSNK2A1 (CSNK2A1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCre-SacB-zeo

Plasmid

#107706

Purpose

Expresses Cre recombinase for excising chromosomal loxP-Hyg-loxP cassette inserts.

Depositor

Kenan Murphy

Publication

Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

Insert

ble (Zeo resistance)

Expression

Bacterial

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pQE31-rsEGFP2

Plasmid

#102879

Purpose

Expresses rsEGFP2 in bacterial cells

Depositor

Stefan Jakobs

Publication

Grotjohann et al eLife. 2012 Dec 31:1:e00248. doi: 10.755

Insert

rsEGFP2

Tags

6xHisTag

Expression

Bacterial

Available since

Jan. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pPMW-attB

Plasmid

#61814

Purpose

pUASp plasmid with attB for Drosophila transgenesis

Depositor

Frederique Peronnet

Publication

Dardalhon-Cumenal et al PLoS Genet. 2018 Jul 11;14(7):e1007498.

Type

Empty backbone

Tags

6xMyc

Expression

Insect

Promoter

UASp

Available since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mTagBFP2-CD81-10

Plasmid

#55281

Purpose

Localization: Tetraspanin/Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Publication

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

CD81 (CD81 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
pDONR223-NME2

Plasmid

#23392

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NME2 (NME2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MAPKAPK2

Plasmid

#23426

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPKAPK2 (MAPKAPK2 Human)

Use

Gateway Cloning

Mutation

P18A

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-MINK1

Plasmid

#23522

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MINK1 (MINK1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pMAL-XPG

Plasmid

#39215

Depositor

Aziz Sancar

Publication

Matsunaga et al J Biol Chem. 1995 Sep 1;270(35):20862-9.

Insert

XPG (ERCC5 Human)

Tags

MBP

Expression

Bacterial

Mutation

Note that XPG starts at amino acid 5

Promoter

Ptac

Available since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

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pDONR223-EPHB4

Plasmid

#23896

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

EPHB4 (EPHB4 Human)

Use

Gateway Cloning

Mutation

K162R

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-BRD4-7A

Plasmid

#90006

Purpose

expresses 7A mutant BRD4

Depositor

Kornelia Polyak

Publication

Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed 484S to 484A, 488S to 488A, 492S to 492A,…

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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minCMV-1xmNG-dMCP

Plasmid

#231091

Purpose

Encodes a 1xmNeonGreen-dMCP under control of a weak promoter (minCMV).

Depositor

John Ngo

Publication

Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

Insert

1xmNG-dMCP

Expression

Mammalian

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_MFE1_hrGFP

Plasmid

#84617

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into MFE1 locus

Depositor

Ian Wheeldon

Publication

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHR_XPR2_hrGFP

Plasmid

#84614

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into XPR2 locus

Depositor

Ian Wheeldon

Publication

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLEX-EF1a ANXA11-mEmerald

Plasmid

#164210

Purpose

pLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoter

Depositor

Jennifer Lippincott-Schwartz

Publication

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mEmerald

Promoter

EF1a

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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