We narrowed to 14,902 results for: EGF

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pLV-H1-shSRGAP2A-hsynapsin-EGFP

Plasmid

#220798

Purpose

shRNA plasmid against human SRGAP2A gene with EGFP expression in neurons

Depositor

Pierre Vanderhaeghen

Article

Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

Insert

EGFP

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1_35S-eGFP-T35S (GB4279)

Plasmid

#225434

Purpose

Transcriptional unit for constitutive expression of the enhanced GFP.

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

35S:eGFP:T35S

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEZYeGFP-SARS-CoV-2E GGG

Plasmid

#224582

Purpose

To express a GFP tagged version of the Envelope protein of SARS-CoV-2 with a mutated PBM in mammalian cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Int J Mol Sci. 2023 Aug 14;24(16):12793.

Insert

SARS-CoV-2E GGG (E SARS-CoV-2)

Tags

GFP

Expression

Mammalian

Mutation

PBM (LLV) mutated to GGG

Promoter

CMV

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-Rac1(P29S)

Plasmid

#224280

Purpose

Cell transfection and expression of Rac1 (P29S)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rac1 (RAC1 Human)

Expression

Mammalian

Mutation

P29S

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-Rac1(G12C)

Plasmid

#224279

Purpose

Cell transfection and expression of Rac1 (G12C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rac1 (RAC1 Human)

Expression

Mammalian

Mutation

G12C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-eGFP-msMMS22L

Plasmid

#221014

Purpose

mammalian expression vector of eGFP tagged mouse MMS22L

Depositor

Daniel Durocher

Article

O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

Insert

MMS22L (Mms22l Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

naturally siResistant to siMMS22L (human) aagacuu…

Promoter

CMV

Available Since

Sept. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pT7-NSP3SA11-F2A-EGFP

Plasmid

#220808

Purpose

Express separately the Rotavirus SA11 NSP3 protein and the original enhanced GFP (Yang et al., 1996)

Depositor

Alex Borodavka

Insert

Rotavirus SA11 NSP3-F2A-EGFP

Use

Other

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-eGFP-shAqp4-4

Plasmid

#223225

Purpose

mir30 based shRNA strategy, shRNA targeting mouse Aqp4

Depositor

Li-Huei Tsai

Article

Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

Insert

shAqp4 (Aqp4 Mouse)

Use

AAV

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-eGFP-shAqp4-3

Plasmid

#223224

Purpose

mir30 based shRNA strategy, shRNA targeting mouse Aqp4

Depositor

Li-Huei Tsai

Article

Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

Insert

shAqp4 (Aqp4 Mouse)

Use

AAV

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-eGFP-shAqp4-1

Plasmid

#223223

Purpose

mir30 based shRNA strategy, shRNA targeting mouse Aqp4

Depositor

Li-Huei Tsai

Article

Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

Insert

shAqp4 (Aqp4 Mouse)

Use

AAV

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHIV-EGFP.Flag-EZH2-mt2*

Plasmid

#220246

Purpose

Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 2* (EZH2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

PRKKKR494-499NAAIRS

Promoter

EF-1α

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pHIV-EGFP.Flag-EZH2-mt1

Plasmid

#220244

Purpose

Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 1 (EZH2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

R27A, R31A, F165A

Promoter

EF-1α

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pKSR1-CA3-GS-EGFP (C-KSR-GS)

Plasmid

#217757

Purpose

Fluorescent reporter for ceramide (mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Kinase suppressor of ras 1 CA3 domain (KSR1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

CA3 domain aa 317-400 with GGSSGGGGA linker

Promoter

CMV

Available Since

June 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5_NLS-Halo-eGFP-BioID

Plasmid

#218448

Purpose

expresses the NLS-Halo-eGFP-BioID fusion protein via stable Flp-In integration

Depositor

Thorsten Stafforst

Article

Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

Insert

NLS-Halo-eGFP-BirA

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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CEL-Frb-EGFP-HOTag3

Plasmid

#208748

Purpose

part A of SparkDrop-Frb

Depositor

Xiaokun Shu

Article

Chung et al ACS Cent Sci. 2023 Jul 7;9(7):1466-1479.

Insert

CEL-Frb-EGFP-HOTag3

Expression

Mammalian

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSCALPS-Flag-Miwi-EGFP

Plasmid

#212575

Purpose

Lentiviral expression of Flag-tagged mouse Miwi in mammalian cells

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

Miwi (Piwil1 Mouse)

Use

Lentiviral

Tags

5x Flag and SNAP tag

Expression

Mammalian

Promoter

SFFV

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-TetR-KRf-TetR

Plasmid

#216270

Purpose

Construct 3: TetR - Lipid binding domain KRφ - TetR

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

TetR - Lipid binding motif (KRf) - TetR

Tags

EGFP

Expression

Mammalian

Available Since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pKH143_EGFP-YIPF3 LIR mut

Plasmid

#214975

Purpose

GFP tagged LIR mutant YIPF3

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

GFP-YIPF3_mut (YIPF3 Human)

Use

Lentiviral

Mutation

LIR mutant

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1 EGFP-LC3B HRD

Plasmid

#207551

Purpose

Homologous recombination donor to integrate and expression cassette for eGFP-LC3B in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible EGFP-LC3B

Tags

EGFP

Expression

Mammalian

Promoter

Endogenous

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1 EGFP-GABARAPL1 HRD

Plasmid

#207552

Purpose

Homologous recombination donor to integrate and expression cassette for EGFP-GABRAPL1 in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible EGFP-GABARAPL1

Tags

EGFP

Expression

Mammalian

Promoter

Endogenous

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec22b-P33-shR

Plasmid

#208359

Purpose

Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b-P33 mutant

Depositor

Paula Nunes-Hasler

Article

Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

Insert

Sec22b (Sec22b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

four silent mutations (shRNA resistance), 33 aa p…

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-ORP8-H514A-H515A

Plasmid

#208360

Purpose

Mammalian expression of fluorescent N-terminally tagged H514A-H515A mutant of ORP8

Depositor

Paula Nunes-Hasler

Article

Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

Insert

ORP8 (OSBPL8 Human)

Tags

EGFP

Expression

Mammalian

Mutation

H514A-H515A

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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psgRNA-GST-EGFP-GPIDAF

Plasmid

#213719

Purpose

To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF under a CMV promoter.

Depositor

Qilai Huang

Article

Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

Insert

EGFP

Use

CRISPR

Tags

GST

Promoter

CMV

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-TetON-mEGFP-MYOD1_WT

Plasmid

#215617

Purpose

For integration of MYOD1 WT T2A mEGFP

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

MYOD1 (MYOD1 Human)

Tags

T2A mEGFP

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-TetON-mEGFP-NGN2_WT

Plasmid

#215610

Purpose

For integration of NGN2 WT T2A mEGFP

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

NGN2 (NEUROG2 Human)

Tags

T2A mEGFP

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-TetON-mEGFP-MYOD1_AroPERFECT-C

Plasmid

#215621

Purpose

For integration of MYOD1 AroPERFECT C T2A mEGFP

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

MYOD1 AroPERFECT C (MYOD1 Human)

Tags

T2A mEGFP

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSCALPS-Flag-Mili-EGFP

Plasmid

#212576

Purpose

Lentiviral expression of Flag-tagged mouse Mili in mammalian cells

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

Mili (Piwil2 Mouse)

Use

Lentiviral

Tags

5x Flag and SNAP tag

Expression

Mammalian

Promoter

SFFV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_06-mEGFP

Plasmid

#194684

Purpose

CMV driven expression of the calcium recorder Caprola_06 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_06-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_04-mEGFP_WRPE-SV40

Plasmid

#194688

Purpose

hSyn1 driven expression of the calcium recorder Caprola_04 fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_04-mEGFP

Use

AAV

Tags

mEGFP

Promoter

hSyn1

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-PARP1-delM43;F44I

Plasmid

#211579

Purpose

CMV driven PARP1-delM43;F44I -eGFP expressing construct with IRES-Neomycin selection

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

Contains deletion of M43 and F44I

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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