We narrowed to 5,172 results for: PEP

1. pTAMAHISTEV_PfeA

   Plasmid

   #128943

   Purpose

   expresses PfeA in E. coli

   Depositor

   James Naismith

   Article

   Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

   Insert

   ferric enterobactin receptor
   

   Use

   Tags

   TEV protease cleavable 7xHis and TamA

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed (1-75)

   Promoter

   T7

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. RH5-bio

   Plasmid

   #47780

   Purpose

   Expresses enzymatically monobiotinylated full-length RH5 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised RH5
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP4-myc-HLA-B8

   Plasmid

   #135512

   Purpose

   Mammalian expression of myc-tagged HLA-B*08:01

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-B*08:01 (HLA-B Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-human NMyc-humanER-IRES-mCherry

   Plasmid

   #73571

   Purpose

   Mammalian retroviral expression of NMyc-ER

   Depositor

   Martine Roussel

   Article

   Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

   Insert

   NMyc (MYCN Human)
   

   Use

   Retroviral

   Tags

   Human ER and IRES-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pNG1256

   Plasmid

   #149710

   Purpose

   Expresses RpoA, RpoB, RpoC-9His and RpoZ from Bacillus subtilis to produce core RNAP complex

   Depositor

   Peter Lewis

   Article

   Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

   Insert

   rpoZ (rpoZ )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCEP4-AD8-QES.i08.c04

   Plasmid

   #123259

   Purpose

   Mammalian expression plasmid for Env from the AD8 HIV-1 isolate; QES mutant for enhanced presentation of quaternary epitopes

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (AD8) Env
   

   Use

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; Mutations P124D, …

   Promoter

   CMV

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Nrxn3α-AS4+

   Plasmid

   #184333

   Purpose

   Expression of mouse Nrx3-alpha containing insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

   Depositor

   Peter Scheiffele

   Article

   Nguyen et al Elife. 2016 Dec 13;5. doi: 10.7554/eLife

   Insert

   Nrxn3-alpha (Nrxn3 Mouse)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOCC304

   Plasmid

   #118897

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with TEV and C-terminal HIS6, cleavable with TEV

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-TwinStrep-MBP-TEV-NotI-ccdB-AscI-TEV-HIS6-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with TEVprotease and TwinStrep-MB…

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-mPCSK8

   Plasmid

   #122677

   Purpose

   Expresses mouse PCSK8 in mammalian cells

   Depositor

   Liming Pei

   Article

   Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

   Insert

   Mus musculus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant 2 (Mbtps1 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. p221z-erVenusYFP

    Plasmid

    #71265

    Purpose

    Entry clone containing ER-localized Venus. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    VenusYFP
    

    Use

    Gateway

    Tags

    ER retention signal HDEL and signal peptide (ER)…

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-myc-H2-Dd

    Plasmid

    #135518

    Purpose

    Mammalian expression of myc-tagged H2-Dd

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    H2-Dd (H2-D1 Mouse)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    C121S

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTarget TMEM97-P2A-mCardinal

    Plasmid

    #157747

    Purpose

    Express in mammalian cells TMEM97 and mCardianl to follow transfection efficiency using flow cytometry. mCardinal is expressed following a P2A skip peptide so it is not connected to TMEM97.

    Depositor

    Andrew Kruse

    Article

    Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

    Insert

    TMEM97 (TMEM97 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. p221z-erTagRFP

    Plasmid

    #71266

    Purpose

    Entry clone containing ER-localized TagRFP. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    TagRFP
    

    Use

    Gateway

    Tags

    ER retention signal HDEL and signal peptide (ER)…

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-PKI nls

    Plasmid

    #118301

    Purpose

    Expression of PKIa

    Depositor

    Philip Stork

    Article

    Billiard et al J Biol Chem. 2001 Aug 17;276(33):31238-4

    Insert

    PKI nls (aa 2-36)
    

    Use

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    inserting 2xnls

    Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. GlycophorinB-CD4d3+4-bio

    Plasmid

    #73094

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding GlycophorinB with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Glycophorin B (GYPB Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCIneo-NHA-Ago2

    Plasmid

    #115361

    Purpose

    Expression vector to produce NHA-tagged human Ago2 protein

    Depositor

    Witold Filipowicz, Ramesh Pillai

    Article

    Pillai et al RNA. 2004 Oct . 10(10):1518-25.

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    N peptide and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. p221k-erTq2CFP

    Plasmid

    #71264

    Purpose

    Entry clone containing ER-localized Turqoise2. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system.

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    Tq2CFP
    

    Use

    Gateway

    Tags

    ER retention signal HDEL and signal peptide (ER)…

    Expression

    Mutation

    Promoter

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOCC119

    Plasmid

    #118884

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
    

    Use

    Tags

    HIS6-MBP, cleavable with 3C protease and mGFP, cl…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a(+) 6xHis-TEV-IFIT3

    Plasmid

    #53559

    Purpose

    bacterial expression of IFIT3

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT3 (IFIT3 Human)
    

    Use

    Tags

    6xHIS and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pEF NS34a-msGFP-A'Q-mito

    Plasmid

    #158763

    Purpose

    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    NS3/4a protease and msGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Flexible linkers between the protease and msGFP a…

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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