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hiSIIN

Plasmid

#185662

Purpose

shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

Depositor

Tyler Jacks

Article

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc

Use

Lentiviral

Promoter

Ef1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pQE32-pr-mEosFP (mEosFP-V69T)

Plasmid

#99213

Purpose

Bacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEosFP

Expression

Bacterial

Mutation

monomeric variant with mutation V69T for primed c…

Promoter

T5

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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midSIIN

Plasmid

#185665

Purpose

shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

Depositor

Tyler Jacks

Article

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc

Use

Lentiviral

Promoter

Ef1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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dimSIIN

Plasmid

#185661

Purpose

shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

Depositor

Tyler Jacks

Article

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc
GFP

Use

Lentiviral

Promoter

Ef1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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Neurl1Rm-Flag pcDNA3.1

Plasmid

#17319

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Use

RNAi

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pEY43

Plasmid

#191047

Purpose

eat-4(prom6-1) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

eat-4(prom6-1) (eat-4 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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mPA-GFP-Actin-7

Plasmid

#57120

Purpose

Localization: Actin, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Article

Olenych et al Curr Protoc Cell Biol. 2007 Sep;Chapter

Insert

Actin (ACTB Human)

Tags

mPA-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pEGFP-GNB1

Plasmid

#133856

Purpose

Mammalian expression of GNB1 N-terminally tagged with EGFP

Depositor

Dave Piston

Article

Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

Insert

GNB1 (GNB1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEF5B-FRT-AP-Smo-YFP-DEST

Plasmid

#49099

Purpose

Expressing AP-Smo-YFP in mammalian cells, can be used for establishing Flp-In stable cell lines

Depositor

Maxence Nachury

Article

Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

Insert

Smo (Smo Mouse)

Tags

YFP and acceptor peptide (AP)

Expression

Mammalian

Promoter

T7

Available Since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only

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EYFP-Mito-7

Plasmid

#56596

Purpose

Localization: Mitochondria, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Mito (COX8A Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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loSIIN

Plasmid

#185663

Purpose

shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

Depositor

Tyler Jacks

Article

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc
GFP

Use

Lentiviral

Promoter

Ef1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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mCyRFP1-RhoA

Plasmid

#84358

Purpose

fusion protein of RhoA, FRET donor

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mCyRFP1-RhoA (RHOA Synthetic, Human)

Tags

mCyRFP1 N terminal fusion to RhoA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A1

Plasmid

#135503

Purpose

Mammalian expression of myc-tagged HLA-A*01:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*01:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEX-CFP-hLC3WT

Plasmid

#24985

Depositor

Isei Tanida

Article

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Insert

a wild type human MAP1-LC3B fused with ECFP (MAP1LC3B Human)

Tags

ECFP

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mEos3.2 (mEosFP3.2-A69T)

Plasmid

#99224

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos3.2 (mEos3.2-A69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos3.2

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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noSIIN

Plasmid

#185666

Purpose

shApc-expressing lentiviruses with no expression of SIINFEKL

Depositor

Tyler Jacks

Article

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc

Use

Lentiviral

Promoter

Ef1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-Narg2

Plasmid

#49247

Purpose

mammalian expression of flag-tagged human Narg2

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

Narg2 (NARG2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only
pFastBac1_GI.1_WT

Plasmid

#162579

Purpose

Expresses norovirus GI.1 VP1 protein in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Promoter

polyhedrin

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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JN302: pMVP/SB/Puro-DEST

Plasmid

#121865

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Puro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEX-GFP-hLC3ΔG

Plasmid

#24988

Depositor

Isei Tanida

Article

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Insert

a mutant human MAP1-LC3B fused with EGFP (MAP1LC3B Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted Gly at 121

Available Since

June 28, 2010

Availability

Academic Institutions and Nonprofits only