We narrowed to 5,115 results for: LucY

1. pGL4.23-WIPI1promotor

   Plasmid

   #206840

   Purpose

   The plasmid contains the human WIPI1 promotor sequence (-1226 bp upstream to +329 bp downstream of the transcription start site) cloned into pGL4.23[luc2/minP] (NheI/HindIII).

   Depositor

   Tassula Proikas-Cezanne

   Article

   Sporbeck et al Commun Biol. 2023 Aug 24;6(1):872. doi:

   Insert

   WIPI1 promotor (partial)
   

   Use

   Luciferase

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTFG027_Spike_sACE2-resistant_D614G_F486S_3xFlag

   Plasmid

   #191572

   Purpose

   Mutated SARS-CoV-2 Spike under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. Encodes F486S mutation. For expression and pseudotyping lentiviral particles.

   Depositor

   Joshua Leonard

   Article

   Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

   Insert

   SARS-CoV-2 Spike Protein (S Synthetic)
   

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   D614G and F486S (confers reduced binding affinity…

   Promoter

   CMV

   Available Since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Lenti-sgRNA-Cre-GpNLuc-sgmMRE11

   Plasmid

   #208384

   Purpose

   Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine MRE11 gene.

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Insert

   sgMRE11 (Mre11a Mouse)
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. cacna2d1 R241A pMT2

   Plasmid

   #206110

   Purpose

   expression of rat alpha2delta-1 calcium channel auxillary subunit and with R421A mutation that disrupts Gabapentin binding

   Depositor

   Annette Dolphin

   Article

   Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

   Insert

   cacna2d1 (Cacna2d1 Rat)
   

   Expression

   Mammalian

   Mutation

   R241A

   Promoter

   Ad MLP/TPL/SV40

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1(+)Gα12-133-NLuc

   Plasmid

   #207156

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA12 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNA12 (GNA12 Human)
   

   Expression

   Mammalian

   Mutation

   NLuc inserted at position A(133)F

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. psicheck2-B4GALT3mut

   Plasmid

   #203602

   Purpose

   Reporter plasmid carrying B4GALT3 3'UTR with mutated miR27b target sequences.

   Depositor

   Hiroyuki Mizuguchi

   Article

   Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

   Insert

   beta-1,4-galactosyltransferase 3 (B4GALT3 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Seed sequences in miR-27b target sequences were m…

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. psicheck2-MAIP1mut

   Plasmid

   #203604

   Purpose

   Reporter plasmid carrying MAIP1 3'UTR with mutated miR27b target sequences

   Depositor

   Hiroyuki Mizuguchi

   Article

   Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

   Insert

   matrix AAA peptidase interacting protein 1 (MAIP1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   miR-27b target sequences in the 3'UTR was mu…

   Promoter

   SV40

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Cav2.2 BBS W391A pcDNA3

   Plasmid

   #206077

   Purpose

   Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop that prevents Beta binding

   Depositor

   Annette Dolphin

   Article

   Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Expression

   Mammalian

   Mutation

   W391A

   Promoter

   CMV

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. lentiTet-CSK-P2A-mCherry BlastR

   Plasmid

   #186742

   Purpose

   Dox inducible CSK-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   CSK (CSK Human)
   

   Use

   Lentiviral

   Tags

   P2A-mCherry

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL3-pSMANTIS_WT_1kb

    Plasmid

    #194187

    Purpose

    Includes the promoter (1kb) of SMTS (SMANTIS, MANTIS, AK125871)

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Promoter

    SMANTIS promoter (1kb)

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ABHD2-3'UTR-Del-1

    Plasmid

    #190095

    Purpose

    examine the activity of ABHD2 3'UTR

    Depositor

    Bowen Liu

    Article

    Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

    Insert

    ABHD2 3' UTR delete-1 (ABHD2 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    deleted the base pairs 6704-6709 refer to NM_0070…

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ABHD2-3'UTR-Del-2

    Plasmid

    #190096

    Purpose

    examine the activity of ABHD2 3'UTR

    Depositor

    Bowen Liu

    Article

    Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

    Insert

    ABHD2 3' UTR delete-2 (ABHD2 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    deleted the base pairs 7122-7129 refer to NM_0070…

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNOC_dCas9_sgRNA Td1

    Plasmid

    #176257

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 1

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    dead spCas9
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNOC_hfnCas12a-Nlux_crRNA NR2

    Plasmid

    #176245

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNOC_hfnCas12a-Nlux_crRNA NR3

    Plasmid

    #176246

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. hsa-cdk2 3'UTR sicheck2 mut

    Plasmid

    #121124

    Purpose

    luciferase assay

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    hsa-cdk2 3'UTR mutated 199 binding (CDK2 Human)
    

    Expression

    Bacterial

    Mutation

    miR-199 seed binding site mutated

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. dre-cdk2 3'UTR sicheck2 199 mut

    Plasmid

    #121122

    Purpose

    luciferase assay

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    dre-cdk2 3'UTR mutated 199 binding (cdk2 Zebrafish)
    

    Expression

    Bacterial

    Mutation

    miR-199 seed binding site mutated

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Gal4-TET1-878-911-I894A(pL84)

    Plasmid

    #124404

    Purpose

    Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with I894A mutation.

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    Gal4-TET1-878-911(I894A mutation) (TET1 Human)
    

    Tags

    gal4 dna binding domain

    Expression

    Mammalian

    Mutation

    I894A

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Gal4-TET1-878-911-T898A(pL84)

    Plasmid

    #124403

    Purpose

    Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with T898A mutation.

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    Gal4-TET1-878-911(T898A mutation) (TET1 Human)
    

    Tags

    gal4 dna binding domain

    Expression

    Mammalian

    Mutation

    T898A

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Gal4-TET1-878-911-L900A(pL84)

    Plasmid

    #124402

    Purpose

    Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L900A mutation.

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    Gal4-TET1-878-911(L900A mutation) (TET1 Human)
    

    Tags

    gal4 dna binding domain

    Expression

    Mammalian

    Mutation

    L900A

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge