We narrowed to 10,970 results for: T7

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Plk1 FRET sensor c-jun substrate

Plasmid

#45203

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

Insert

Plk1 FRET sensor with c-jun substrate

Tags

CyPET, YPET

Expression

Mammalian

Promoter

T7

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-Nup37-EGFP-polyA

Plasmid

#104562

Purpose

Express Nup37-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Nup37 (Nup37 Mouse)

Tags

EGFP tag

Expression

Mammalian

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-HiBiTGFPHis

Plasmid

#188317

Purpose

Expression of HiBiT-GFP via T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

HiBiT-GFP fusion

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Promoter

T7Max

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET A-mEosEM-E

Plasmid

#226336

Purpose

Photoswitchable fluorescent protein for correlative light-electron microscopy; mEosEM-E for prokaryotic expression

Depositor

Pingyong Xu

Article

Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

Insert

mEosEM-E

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET24b-BG1.3

Plasmid

#199437

Purpose

expresses BAmGreen1.3 in bacterial cells

Depositor

Andrew Woolley

Article

Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

Insert

BAMGreen1.3

Tags

his tag

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pYFJ84

Plasmid

#227644

Purpose

Vibrio harveyi acyl-ACP synthetase

Depositor

John Cronan

Article

Jiang et al Biochemistry. 2006 Aug 22;45(33):10008-1

Insert

Acyl-Acyl carrier protein synthetase

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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wt HIV-1 integrase in pET15b

Plasmid

#61668

Purpose

wild type HIV-1 integrase in pET-15b

Depositor

Robert Craigie

Article

Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

Insert

wild type HIV-1 integrase

Tags

His

Promoter

T7 promotor

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only
pET28a-HisYFP-Sp100

Plasmid

#53141

Purpose

E.coli expression of HisYFP tagged human Sp100 fragment

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

Sp100A fragment (SP100 Human)

Tags

His-YFP

Expression

Bacterial

Mutation

fragment (amino acids 71-480); deletion of L75

Promoter

T7

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huErCas12a

Plasmid

#174685

Purpose

Expresses 6xHis-MBP tagged ErCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huErCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV, NLS and NLS, 6xHis

Expression

Bacterial

Mutation

Q926R mutation is observed as indicated from the …

Promoter

T7

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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EcRho

Plasmid

#113121

Purpose

Expresses E. coli Rho

Depositor

James Berger

Article

Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

Insert

Rho

Tags

MGH

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pKDC.109

Plasmid

#227189

Purpose

T7 IPTG-driven Cas9

Depositor

Seth Shipman

Article

Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

Insert

SpyCas9

Expression

Bacterial

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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luxB:Venus+luxA/pRSETB Duet

Plasmid

#197582

Purpose

BRET lux luciferase with Venus for bacterial expression

Depositor

Takeharu Nagai

Article

Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

Insert

LuxA LuxBVenus

Use

E.coli expression

Tags

His X 6

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRSETA_crE6

Plasmid

#172928

Purpose

Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 1 helix and 2 GSG linkers

Depositor

Arnold Boersma

Article

Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

Insert

CrowdingSensorE6

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET_BGL3

Plasmid

#101912

Purpose

Expresses a β-glucosidase gene from Rhodothermus marinus in E. coli under the control of the pET system.

Depositor

Steve Shih

Article

Husser et al ACS Synth Biol. 2018 Mar 16;7(3):933-944

Insert

β-glucosidase

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7+LacO

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFN18A-HaloTag-SpyCatcher

Plasmid

#206041

Purpose

HaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTag

Depositor

Sergi Garcia-Manyes

Article

Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

Type

Empty backbone

Tags

HaloTag and SpyCatcher-8His

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pDM_T7_HisLacZomega

Plasmid

#118815

Purpose

T7 RNAP-driven expression of N-terminal His-tagged lacZ omega subunit.

Depositor

Alexander Green

Article

Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

Insert

lacZ_

Tags

histag

Expression

Bacterial

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-RILP

Plasmid

#74600

Purpose

used for expression in E. coli and purification

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

Rab-interacting lysosomal protein (RILP Human)

Tags

FLAG, StrepII, and superfolder GFP

Expression

Bacterial

Promoter

T7

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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6his-WT gusA-pET28a+

Plasmid

#61156

Purpose

N -terminal His tagged wild-type GUS expression vector

Depositor

Andrew Ellington, Ichiro Matsumura

Article

Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

Insert

gusA

Tags

6x His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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ReNL_pRSETb

Plasmid

#89536

Purpose

Red color variant of bright luminescent protein for bacterial expression

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Tags

His X 6

Expression

Bacterial

Promoter

T7

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcNB1

Plasmid

#89241

Purpose

Resurfaced Cell-penetrating GFP nanobody

Depositor

Brian McNaughton

Article

Bruce et al Protein Sci. 2016 Jun;25(6):1129-37. doi

Insert

pcNB1 (GFP)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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