We narrowed to 11,339 results for: UTY

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pEx-insertion-erm-T1T2

Plasmid

#172212

Purpose

For genetic insertion in erythromycin-sensitive Prevotella copri strains, with T1-T2 terminator

Depositor

Till Strowig

Article

Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

Type

Empty backbone

Use

Genetic insertion in prevotella copri

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCF CREB M1

Plasmid

#22969

Depositor

Marc Montminy

Article

Du et al Mol Cell Biol. 2000 Jun . 20(12):4320-7.

Insert

CREB (Creb1 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

Changed Serine 133 to Alanine

Available Since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

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mOtop1_pcDNA3

Plasmid

#114677

Purpose

This construct was used to express mouse Otop1 in HEK-293 cells

Depositor

Emily Liman

Article

Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

Insert

Otopetrin 1 (Otop1 Mouse)

Expression

Mammalian

Mutation

SNP (rs49325632): Glycine 396 to Alanine

Promoter

CMV

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pZZMP26

Plasmid

#231755

Purpose

Mammalian expression of TOP6BL

Depositor

Scott Keeney

Article

Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

Insert

TOP6BL (Gm960 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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APneg-VAMP1b

Plasmid

#168008

Purpose

negative selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

VAMP1(T29-K89)-T7PAP

Expression

Bacterial

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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APneg-VAMP1c

Plasmid

#168009

Purpose

negative selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

VAMP1(T29-K89)-T7PAP

Expression

Bacterial

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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ACPPiso1-V5

Plasmid

#113052

Purpose

Expresses human prostatic acid phosphatase (isoform 1) in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

Insert

prostatic acid phosphatase isoform 1 (ACP3 Human)

Tags

V5

Expression

Mammalian

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pJH645

Plasmid

#172169

Purpose

Reporter plasmid for pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Pem7 luxCDA(B.1TAG)E; Plpp pylS; PproK pylT

Expression

Bacterial

Mutation

Inserted amber codon immediately after initiator …

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pPy-CAG-Cre::ERT2-IRES-BSD

Plasmid

#48760

Purpose

CAG promoter driving Cre-ERT2 fusion-IRES-Blasticidin resistance

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Insert

Cre::ERT2

Use

Ppy

Tags

ERT2

Expression

Mammalian

Promoter

CAG

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pEX.d14GFP.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

Plasmid

#45564

Purpose

Expresses I-Sce I nuclease and BFP tracker in mammalian cells, codes for eGFP donor sequence

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Inserts

d14 eGFP donor
I-Sce I Nuclease
BFP

Tags

HA

Expression

Mammalian

Mutation

deleted n' 15 amino acids

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pET-X(5010)B1

Plasmid

#168019

Purpose

Expresses X(5010)B1-6xHis in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

X(5010)B1-6xHis

Tags

Hisx6

Expression

Bacterial

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5

Plasmid

#135454

Purpose

Bacterial expression of TEVΔ protease

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5

Tags

MBP, His6

Expression

Bacterial

Mutation

S219V mutation improves stability. Truncated form…

Promoter

tac promoter

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pSin_EF1a_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR

Plasmid

#176310

Purpose

Expresses CreN59-nMag-NLS with mCherry marker.

Depositor

Yingxiao Wang

Article

Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

Insert

CreN59_nMag_NLS_P2A_mCherry_IRES_puroR

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

human EF1alpha

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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Ubiquitin K48C

Plasmid

#86588

Purpose

Bacterial expression of Ubiquitin K48C for cross linking studies

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

Ubiquitin (UBC Human)

Tags

His6-MBP

Expression

Bacterial

Mutation

TEV site; K48C

Promoter

Tac

Available Since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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ACPPiso2-V5

Plasmid

#113053

Purpose

Expresses human prostatic acid phosphatase (isoform 2) in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

Insert

prostatic acid phosphatase isoform 2 (ACP3 Human)

Tags

V5

Expression

Mammalian

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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His6-PRC1

Plasmid

#69111

Purpose

For bacterial expression of recombinant human PRC1 tagged with His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Protein Regulator of Cytokinesis 1 (PRC1 Human)

Tags

His6

Expression

Bacterial

Available Since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCMU-LEr

Plasmid

#61185

Purpose

Fluorescent reporter for late endosome mCherry-MtRAB5A2 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB5A2

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pJB042 (FtsZ-mEos2)

Plasmid

#49764

Purpose

Inducible expression of FtsZ-mEos2 in bacteria

Depositor

Jie Xiao

Article

Buss et al Mol Microbiol. 2013 Sep;89(6):1099-120.

Insert

FtsZ-mEos2

Tags

mEos2

Expression

Bacterial

Promoter

T5-lac

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-BE4max-BlastR

Plasmid

#194061

Purpose

Mammalian expression of evoCjCas9 CBE4max

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-BE4max

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMUT2-pTcpP-mCherry

Plasmid

#192861

Purpose

pMUT2 based vector for sensing human primary bile acids in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

TcpP-CadC fusion protein and cognate promoter inducible by human primary bile acids

Expression

Bacterial

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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