We narrowed to 6,585 results for: arl
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Plasmid#215547PurposegRNA targeting B2M to introduce splice donor mutation on the first intron of the locusDepositorInsertB2M (B2M Human)
UseCRISPRAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
INS-3'gRNA
Plasmid#210468PurposegRNA targeting INS 3' terminal for CRISPR-Cas9-mediated knock-inDepositorInsertINS (INS Human)
UseCRISPRAvailable SinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003(M115G)-Talin rod-mCherry
Plasmid#210026PurposeExpression of SpyTag003(M115G)-Talin rod(434-2541)-mCherry in mammalian cellsDepositorInsertSpyTag003(M115G)-Talin1 rod domain(434-2541)-mCherry
ExpressionMammalianMutationSpyTag003 M115G mutation to modify SpyCatcher003 …PromoterCMVAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003(M115A)-Talin rod-mCherry
Plasmid#210025PurposeExpression of SpyTag003(M115A)-Talin rod(434-2541)-mCherry in mammalian cellsDepositorInsertSpyTag003(M115A)-Talin1 rod domain(434-2541)-mCherry
ExpressionMammalianMutationSpyTag003 M115A mutation to modify SpyCatcher003 …Available SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003(V114T, V116T)-Talin rod-mCherry
Plasmid#210027PurposeExpression of SpyTag003(V114T, V116T)-Talin rod(434-2541)-mCherry in mammalian cellsDepositorInsertSpyTag003(V114T,V116T)-Talin1 rod domain(434-2541)-mCherry
ExpressionMammalianMutationSpyTag003 V114T and V116T mutations to modify Spy…PromoterCMVAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Talin head-SpyCatcher003(K31TAG)
Plasmid#210023PurposeExpression of EGFP-Talin head(1-433)-SpyCatcher003(K31TAG) in mammalian cellsDepositorInsertEGFP-Talin head (1-433)-SpyCatcher003(K31TAG)
ExpressionMammalianMutationSpyCatcher003 with K31TAG (Amber stop codon) muta…PromoterCMVAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET19-TdTc253
Plasmid#207464PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 253DepositorInsertMTdTc253 (Dntt Mouse)
Tags10xHisExpressionBacterialMutationSer253Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…Available SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET19-TdTc188
Plasmid#207463PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 188DepositorInsertTdTc188 (Dntt Mouse)
Tags10xHisExpressionBacterialMutationCys216Ser, Cys302Ala, Cys378Ala, and Cys438SerAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET19-TdTc180
Plasmid#207462PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 180DepositorInsertTdTc180 (Dntt Mouse)
Tags10xHisExpressionBacterialMutationGlu180Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…Available SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET19-TdTc302
Plasmid#207461PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302DepositorInsertTdTc302 (Dntt Mouse)
Tags10xHisExpressionBacterialMutationCys188Ala, Cys216Ser, Cys378Ala, and Cys438SerAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_023d-sgCiPOLR2D
Plasmid#202445PurposeExpression of a CRISPRi guide targeting POLR2DDepositorAvailable SinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003-sgUBR4
Plasmid#202442PurposeExpression of a guide RNA targeting UBR4DepositorAvailable SinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003-sgUBA6
Plasmid#202440PurposeExpression of a guide RNA targeting UBA6DepositorAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN554
Plasmid#177361PurposeExpresses human KIF1A(1-393) fused with leucine zipper, mScarlet and StrepII tagDepositorInsertKIF1A (KIF1A Human)
TagsLeucine zipper, StrepII tag, and mScarletExpressionBacterialPromoterT7Available SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
LB001: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#2 gRNA
Plasmid#131757PurposepMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expressionDepositorInsertTRE#2 Sp/xCas9 gRNA
UseGateway Cloning and Synthetic BiologyPromoterhU6Available SinceOct. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
LA901: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#1 gRNA
Plasmid#131756PurposepMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expressionDepositorInsertTRE#1 Sp/xCas9 gRNA
UseGateway Cloning and Synthetic BiologyPromoterhU6Available SinceOct. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
JV201: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#1 gRNA
Plasmid#131755PurposepMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expressionDepositorInsertTRE#1 SaCas9 gRNA
UseGateway Cloning and Synthetic BiologyPromoterhU6Available SinceOct. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
LB101: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#2 gRNA
Plasmid#131758PurposepMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expressionDepositorInsertTRE#2 SaCas9 gRNA
UseGateway Cloning and Synthetic BiologyPromoterhU6Available SinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-cpVenus-FLARE-EKAR-EV(T/A)
Plasmid#123340PurposeNegative control mutant for Venus-cpVenus-FLARE-EKAR-EV biosensor.DepositorInsertVenus-cpVenus-FLARE-EKAR-EV(T/A)
Tags6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-cpVenus-FLARE-CKAR(T/A)
Plasmid#123344PurposeNegative control mutant for Venus-cpVenus-FLARE-CKAR biosensor.DepositorInsertVenus-cpVenus-FLARE-CKAR(T/A)
Tags6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only