We narrowed to 14,138 results for: eng

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pHiUGE smFP-V5 donor ORF1

Plasmid

#200381

Purpose

Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE smFP-V5 donor ORF2

Plasmid

#200382

Purpose

Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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LOV-Turbo-His6_pET21a

Plasmid

#199668

Purpose

expresses LOV-Turbo in bacteria

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Tags

His6

Expression

Bacterial

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBTK1006 (pSL5)

Plasmid

#191001

Purpose

Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

Depositor

Jeffrey Barrick

Article

Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

Insert

red chromoprotein

Use

Synthetic Biology

Expression

Bacterial

Promoter

CP25

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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SPLINTR GFP v1 backbone

Plasmid

#179777

Purpose

SPLINTR backbone vector

Depositor

Mark Dawson

Article

Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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PA-Dre

Plasmid

#169706

Purpose

Photoactivatable Dre recombinase for AAV production and expression in mammalian cells

Depositor

Dali Li

Article

Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

Insert

Dre246-nMag-T2A-pMag-Dre247

Use

AAV

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ1149 - intron 3 smu-2 PATCs

Plasmid

#161516

Purpose

PATC-rich intron number three from smu-2 to stimulate germline expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron 3 from smu-2

Expression

Worm

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR

Plasmid

#136382

Purpose

CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion protein

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b (D570A)-TV-T2A-MCP-VPR

Use

CRISPR

Expression

Plant

Mutation

D570A

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCAS9-TPC

Plasmid

#61478

Purpose

Binary expression vector with A.th. codon-optimized Cas9 for conventional cloning

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

Cas9

Use

CRISPR

Expression

Plant

Promoter

PcUbi4-2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

Plasmid

#98973

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 2 and Cas9(D10A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pBW103ParsR-Amp30C

Plasmid

#78638

Purpose

pBW100ParsR carrying 30hrpR-30hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

30hrpR-30hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pTXB1-Tn5-F

Plasmid

#160081

Purpose

Bacterial expression of C-terminally FLAG-tagged Tn5 transposase

Depositor

Pier Pandolfi

Article

Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

Insert

Tn5 transposase

Tags

FLAG and Mxe intein - Chitin-binding domain

Expression

Bacterial

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCBEclos-cbei2385-g2-opt

Plasmid

#118215

Purpose

CRISPR-mediated base editing in Clostridium

Depositor

Sheng Yang

Article

Li et al Biotechnol Bioeng. 2019 Feb 10. doi: 10.

Insert

Apobec1, Casp nickase, UG1-sgRNA

Expression

Bacterial

Promoter

Ptm

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR221-JUN

Plasmid

#79512

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human JUN CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

JUN (JUN Human)

Use

Gateway Cloning

Tags

HA Tag

Promoter

no Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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994 pSG5L HA PTEN T366A

Plasmid

#10788

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

T366A

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pCMV-SpRYc-ABE8e-EGFP

Plasmid

#208340

Purpose

Expresses SpRYc-ABE8e in mammalian cells

Depositor

Pranam Chatterjee

Article

Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

Insert

ecTadA(8e)-nSpRYc

Use

CRISPR

Expression

Mammalian

Mutation

Sc++ (1-1119), SpRY (1111-1368), D10A

Promoter

CMV

Available Since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pTMB304_ZFcharm_Kv1_Prnp_SCR

Plasmid

#220842

Purpose

AAV genome expressing ZFcharm Kv1 targeting Prnp; scrambled self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv1

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTMB301_ZFcharm_Kv1_Prnp_SPM

Plasmid

#220840

Purpose

AAV genome expressing ZFcharm Kv1 targeting Prnp; single perfect match self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv1

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-H2-Kb SCT-T2A-GFP

Plasmid

#205454

Purpose

lentiviral plasmid for expression of mouse H2-Kb SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

H2-Kb SCT (H2-K1 Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pHR V5-RNF25_IRES-mCherry

Plasmid

#198386

Purpose

RNF25 expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RNF25 (RNF25 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1A

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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