We narrowed to 17,501 results for: puro

1. psBIG1a ActBT2ATetP2AP - H

   Plasmid

   #229972

   Purpose

   Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and Hygro

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Insert

   ActBT2ATetP2AP - H
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. psBIG1a ActBT2AmcherryP2AP -TagBFPH

   Plasmid

   #229971

   Purpose

   Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains Last ActB exon fused T2A-mCherry-P2A-puro and mTagBFPnls-T2A-Hygro

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Insert

   ActBT2AmcherryP2AP -TagBFPH
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-TRE-TurboID-HA-rtTA

   Plasmid

   #226995

   Purpose

   Backbone plasmid to express TurboID fusion proteins in mammalian cells

   Depositor

   Bruno Dallagiovanna

   Article

   Gomes-Junior et al PLoS One. 2025 May 30;20(5):e0324779. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiGuide-dsRed

   Plasmid

   #217006

   Purpose

   lentiviral plasmid for gRNA cloning with selectable dsRed expression

   Depositor

   Deepta Bhattacharya

   Article

   D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SOX2-3'gRNA

   Plasmid

   #215543

   Purpose

   gRNA targeting SOX2 3' terminal for CRISPR-Cas9-mediated knock-in

   Depositor

   Kai Wang

   Insert

   SOX2 (SOX2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. CJ26 (miR-1003 3'ssAG->GA)

   Plasmid

   #17765

   Depositor

   David Bartel

   Article

   Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

   Insert

   dme-miR-1003 minigene 3'ssAG->GA
   

   Use

   Drosophila metallothionein gene promoter drives e…

   Tags

   Expression

   Insect

   Mutation

   splice site mutation

   Promoter

   Available since

   April 15, 2008

   Availability

   Academic Institutions and Nonprofits only

7. MXS Chaining Kit

   Plasmid Kit

   #1000000064

   Purpose

   MXS-chaining system for assembly of constructs optimized for synthetic biology applications in mammalian systems. 14 different fluorescent proteins, 10 mammalian promoters/enhancers, 3 polyA signals

   Depositor

   Pierre Neveu

   Available since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1-ArchT-BFP2-TSERex

   Plasmid

   #123312

   Purpose

   Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

   Depositor

   Yulong Li

   Article

   Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

   Insert

   ArchT-BFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1 GFP shRNA

   Plasmid

   #30323

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   GFP shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe-Kras G12C

    Plasmid

    #58901

    Purpose

    Expresses human Kras with a G12C mutation

    Depositor

    Channing Der

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G12C mutation

    Promoter

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBabe-Kras G12D

    Plasmid

    #58902

    Purpose

    Expresses human Kras with a G12D Mutation

    Depositor

    Channing Der

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G12D mutation

    Promoter

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MscL(G22S)61-cpGFP-ERexp

    Plasmid

    #195311

    Purpose

    Fluorescent reporter/biosensor for membrane tension

    Depositor

    Allen Liu

    Article

    Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

    Insert

    MscL(G22S)61-cpGFP-ERexp
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX459_gRNA pAS dual_hspCas9

    Plasmid

    #193312

    Purpose

    Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    not applicable

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBabe-Kras S17N

    Plasmid

    #58899

    Purpose

    Expresses human Kras with a S17N mutation

    Depositor

    Channing Der

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S17N mutation

    Promoter

    Available since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX459-CDKN1A

    Plasmid

    #217457

    Purpose

    For CDKN1A (p21) knockout

    Depositor

    John Diffley

    Article

    Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

    Insert

    CRISPR Cas9 guide for CDKN1A
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. plvx-tetO-GFP

    Plasmid

    #191560

    Purpose

    Inducible expression of GFP

    Depositor

    Joseph Sodroski, Alon Herschhorn

    Article

    Herschhorn et al PLoS One. 2011;6(11):e26731. doi: 10.137

    Insert

    gfp
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX459_gRNA-ROSA26_hspCas9

    Plasmid

    #193310

    Purpose

    Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    not applicable

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Flag-HA-OTUB1

    Plasmid

    #22551

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    OTUB1 (OTUB1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hAur-A D274A

    Plasmid

    #8511

    Depositor

    Joan Ruderman

    Article

    Crane et al J Cell Sci 2004 Dec 1;117(Pt 25):5975-83

    Insert

    hAur-A (AURKA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    D274A, catalytically dead mutant

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCIP

    Plasmid

    #79009

    Purpose

    For expression in mouse ESCs or other eukaryotic cells. Contains pCAGGS-IRES-Puro-bpA, with a NheI-MfeI cloning site in between pCAGGS and IRES.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGs

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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