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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,193 results for: puro

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Showing: 4141 - 4160 of 18193 results

  8. pPB9P3

    Plasmid
    #210034
    Purpose
    Contains cloning site for PE3/3b prime editing gRNA constructs, inducible prime editor 2, rtTA-T2A-puro
    Depositor
    Michael Talkowski
    Article
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Inserts
    prime editor 2
    rtta-T2A-puro
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    EF-1α and TRE3G
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210034-plasmid-map-sequence-id-414000

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  10. MSCV-PIG-N3FLAG-TAF12

    Plasmid
    #105593
    Purpose
    retrovirally express mouse TAF12 with 3*FLAG tag at N terminal, GFP marker and puro resistance
    Depositor
    Christopher Vakoc
    Article
    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.
    Insert
    full length TAF12 with silent mutations, making it resistant to TAF12 shRNA#364 (Taf12 Mouse)
    Use
    Retroviral
    Tags
    3*FLAG
    Expression
    Mammalian
    Mutation
    silent mutations to make it resistant to the targ…
    Promoter
    MSCV-LTR
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    105593-plasmid-map-sequence-id-209432

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  11. pLentiV2-dCas9-VP64

    Plasmid
    #141104
    Purpose
    Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa
    Depositor
    Igor Ulitsky
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    141104-plasmid-map-sequence-id-298612

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  12. pLKO-sh-BNIP3-FL

    Plasmid
    #100771
    Purpose
    Lentiviral expression of shRNA targeting BNIP3
    Depositor
    Joseph Gordon
    Insert
    Lenti-sh-BNIP3-FL
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    Sept. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    100771-plasmid-map-sequence-id-193107

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  13. pLV CMV FST-HA

    Plasmid
    #175168
    Purpose
    Lentiviral expression of human FST-HA
    Depositor
    Larry Gerace
    Insert
    FST
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Available Since
    Jan. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    175168-plasmid-map-sequence-id-352511

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  14. pRosetta

    Plasmid
    #59700
    Purpose
    Lentiviral vector for use as a standard for titering experiments when trying to compare vectors with different selection markers or to determine appropriate antibiotic conditions.
    Depositor
    John Doench, David Root
    Inserts
    Puro
    BlastR
    EGFP
    Use
    Lentiviral
    Tags
    2A
    Expression
    Mammalian
    Available Since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    59700-plasmid-map-sequence-id-140560

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  15. pGGSG-FKBP-2HA-P2a-Pac

    Plasmid
    #235521
    Purpose
    C-term tagging construct: clone into PmlI and AfeI sites
    Depositor
    Brian Hendrich
    Insert
    FKBP
    Use
    Mouse Targeting
    Tags
    FKBP, 2xHA, Puro
    Mutation
    None
    Available Since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    235521-plasmid-map-sequence-id-474041

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  16. CROP-seq-opti-dsRed

    Plasmid
    #201999
    Purpose
    Based on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.
    Depositor
    Daniel Geschwind
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    dsRed
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    201999-plasmid-map-sequence-id-398468

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  17. pGGSG-Bromo-T7-T2a-Pac

    Plasmid
    #235518
    Purpose
    Cloning vector for GGSG-BromoTag-T7-T2A-Pac. Clone into PmlI and AfeI
    Depositor
    Brian Hendrich
    Insert
    BromoTag
    Use
    Mouse Targeting
    Tags
    BromoTag, T7, Puro
    Mutation
    None
    Available Since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    235518-plasmid-map-sequence-id-474042

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  18. pGGSG-Bromo-T7-P2a-Pac

    Plasmid
    #235517
    Purpose
    Cloning vector for GGSG-BromoTag-T7-P2A-Pac. Clone into PmlI and AfeI
    Depositor
    Brian Hendrich
    Insert
    BromoTag
    Use
    Mouse Targeting
    Tags
    BromoTag, T7, Puro
    Mutation
    None
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    235517-plasmid-map-sequence-id-473996

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  19. pLV-EF1a-V5-Tmem128

    Plasmid
    #120239
    Purpose
    Expresses V5-tagged Tmem128 in lentiviral vector
    Depositor
    Larry Gerace
    Insert
    Tmem128 (Tmem128 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Promoter
    EF1a
    Available Since
    Jan. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    120239-plasmid-map-sequence-id-232808

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  20. pLEX_307

    Plasmid
    #41392
    Purpose
    Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag
    Depositor
    David Root
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41392-plasmid-map-sequence-id-331248

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Showing: 4141 - 4160 of 18193 results