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UbC-(mNeonGreen)4-tDeg

Plasmid

#129404

Purpose

(mNeonGreen)4-tDeg fluorogenic protein

Depositor

Samie Jaffrey

Publication

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

(mNeonGreen)4-tDeg

Expression

Mammalian

Promoter

UbC

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pHel2

Plasmid

#102960

Purpose

E.coli/H.Pylori shuttle vector

Depositor

Rainer Haas

Publication

Heuermann et al Mol Gen Genet. 1998 Mar;257(5):519-28.

Type

Empty backbone

Use

Plasmid shuttle vector system for genetic complem…

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223-TRPM7

Plasmid

#23476

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TRPM7 (TRPM7 Human)

Use

Gateway Cloning

Mutation

Insert encodes NCBI: NR_149154.2 bases 265 to 146…

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pIVM_26

Plasmid

#204501

Purpose

Expression of EloB (1-104) and EloC (17-112) for production of VCB complexes in conjunction with plasmid pIVM_02 (VHL 54-213)

Depositor

Alessio Ciulli

Publication

Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

Insert

EloB (1-104) and EloC (17-112) (ELOC, ELOB Human)

Expression

Bacterial

Mutation

0

Promoter

AmpR

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCFJ782 - HygroR

Plasmid

#190933

Purpose

Hygromycin resistance co-injection marker

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Hygromycin resistance gene

Expression

Worm

Promoter

rps-0

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTet-O-Nd1-puro

Plasmid

#52052

Purpose

Expresses Nd1 and puromycin resistent gene under control of TetON promoter

Depositor

Marius Wernig

Publication

Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

Insert

Neurod1 (Neurod1 Mouse)

Use

Lentiviral

Tags

T2A-and puromycin resistant cassette

Expression

Mammalian

Promoter

tet-on

Available since

April 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBT268_(pCA-ATG-intron-tTA2-iiTRE-tdT3Mycii)

Plasmid

#36880

Depositor

Liqun Luo

Publication

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

tTA2
tdT-3Myc
insulator

Tags

3 Myc tags

Expression

Mammalian

Mutation

insertion of a beta-globin intron with a loxP sit…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-ADRBK1

Plasmid

#23835

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ADRBK1 (GRK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pGF1-4eCOL2A1

Plasmid

#97210

Purpose

Lentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoter

Depositor

Ryan Porter

Publication

De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d

Insert

4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

COL2A1 regulatory elements

Available since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTTCC

Plasmid

#164264

Purpose

Expresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilus

Depositor

Gudmundur Hreggvidsson

Publication

Adalsteinsson et al Sci Rep. 2021 May 5;11(1):9586. doi: 10.

Inserts

Cas9
gRNA scaffold

Expression

Bacterial

Promoter

Pcel and trc

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-3HA-kanMX6

Plasmid

#39295

Depositor

Jurg Bahler, John Pringle

Publication

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

KanR

Use

Yeast genomic targeting

Tags

3HA, A. gossypii translation elongation factor 1a…

Available since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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MDH1-PGK-GFP microRNA-10b

Plasmid

#16070

Depositor

Bob Weinberg

Publication

Ma et al Nature. 2007 Oct 11. 449(7163):682-8.

Insert

microRNA-10b (MIR10B Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pFG4

Plasmid

#87251

Purpose

gene for resistance to hygromycin (Hyg) inserted next to the parS segments (INT). Also known as pANCH2-Hph

Depositor

Kerstin Bystricky

Publication

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

Hyg

Tags

parS segments

Expression

Yeast

Promoter

TEF

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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hsgk1

Plasmid

#83432

Purpose

full length human sgk1 with Flag tag

Depositor

Christie Thomas

Publication

Raikwar et al Am J Physiol Renal Physiol. 2008 Nov;295

Insert

human Sgk1 (SGK1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pMDC123SB-AtTAS1c-B/c

Plasmid

#51772

Purpose

Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor

Depositor

James Carrington

Publication

Carbonell et al Plant Physiol. 2014 Mar 19.

Insert

AtTAS1c-B/c

Use

Plant expression

Mutation

A. thaliana TAS1c precursor sequence including a …

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTol1-UAS:ChrimsonR-tdTomato

Plasmid

#124231

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChrimsonR fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Publication

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

ChrimsonR-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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betaRBPIG

Plasmid

#16422

Depositor

Juergen Hescheler

Publication

Pfannkuche et al Nat Protoc. 2008. 3(9):1510-26. doi: htt

Insert

loxP flanked RFP and blasticidin-resistance plus puromycin-acetyltransferase IRES GFP

Expression

Mammalian

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pTYB3-PUM1-HD

Plasmid

#17543

Depositor

Traci Hall

Publication

Wang et al Mol Cell. 2001 Apr . 7(4):855-65.

Insert

human PUM1 Pumilio homology domain (PUM1 Human)

Tags

intein

Expression

Bacterial

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFP

Plasmid

#90015

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFP

Depositor

Julien Béthune

Publication

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Ago2
NBirA*-GFP

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRK5-HA-UFM1

Plasmid

#134639

Purpose

expression of HA-tagged human UFM1 wildtype

Depositor

Yihong Ye

Publication

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFM1 (UFM1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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