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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,238 results for: 272

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Showing: 4161 - 4180 of 4238 results

  1. TLP-bio

    Plasmid
    #47787
    Purpose
    Expresses enzymatically monobiotinylated full-length TLP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised TLP
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47787-plasmid-map-sequence-id-68823

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  3. pET28-His10-BTLAicd (AA190-289, Y243F, Y257F, Y282F)-TwinStrep

    Plasmid
    #180806
    Purpose
    Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y243F, Y257F, Y282F)-TwinStrep
    Depositor
    Enfu Hui
    Article
    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Insert
    BTLAint (AA190-289, Y243F, Y257F, Y282F)-TwinStrep (BTLA Human)
    Expression
    Bacterial
    Mutation
    BTLA (Y243F, Y257F, Y282F)
    Promoter
    T7
    Available Since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    180806-plasmid-map-sequence-id-356797

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  4. pET28-His10-BTLAicd (AA190-289, Y226F, Y257F, Y282F)-TwinStrep

    Plasmid
    #180807
    Purpose
    Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y257F, Y282F)-TwinStrep
    Depositor
    Enfu Hui
    Article
    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Insert
    BTLAint (AA190-289, Y226F, Y257F, Y282F)-TwinStrep (BTLA Human)
    Expression
    Bacterial
    Mutation
    BTLA (Y226F, Y257F, Y282F)
    Promoter
    T7
    Available Since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    180807-plasmid-map-sequence-id-356795

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  5. pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y282F)-TwinStrep

    Plasmid
    #180808
    Purpose
    Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y282F)-TwinStrep
    Depositor
    Enfu Hui
    Article
    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Insert
    BTLAint (AA190-289, Y226F, Y243F, Y282F)-TwinStrep (BTLA Human)
    Expression
    Bacterial
    Mutation
    BTLA (Y226F, Y243F, Y282F)
    Promoter
    T7
    Available Since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    180808-plasmid-map-sequence-id-356794

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  6. pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y257F)-TwinStrep

    Plasmid
    #180809
    Purpose
    Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y257F)-TwinStrep
    Depositor
    Enfu Hui
    Article
    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Insert
    BTLAint (AA190-289, Y226F, Y243F, Y257F)-TwinStrep (BTLA Human)
    Expression
    Bacterial
    Mutation
    BTLA (Y226F, Y243F, Y257F)
    Promoter
    T7
    Available Since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    180809-plasmid-map-sequence-id-356793

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  7. NSS-GFP

    Plasmid
    #163661
    Purpose
    Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from TNF and TMD and luminal domain from ST6GAL1 in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the cytosolic tail from TNF…
    Available Since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163661-plasmid-map-sequence-id-322793

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  8. SNN-GFP

    Plasmid
    #163664
    Purpose
    Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, and TMD and luminal domain from TNF in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the cytosolic tail from ST,…
    Available Since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163664-plasmid-map-sequence-id-322797

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  9. NNS-GFP

    Plasmid
    #163666
    Purpose
    Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail and TMD from TNF, and luminal domain from ST6GAL1 in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the cytosolic tail from TNF…
    Available Since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163666-plasmid-map-sequence-id-322799

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  13. p28-2iDMD-puro

    Plasmid
    #88895
    Purpose
    28°C full-length DMD luc-puro-red plasmid. Co-expresses human DMD, firefly luciferase, puroR and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 37°C.
    Depositor
    Michele Calos
    Article
    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/
    Inserts
    Dystrophin (DMD Human)
    luciferase
    puromycin resistance enzyme
    mCherry
    Expression
    Mammalian
    Mutation
    codon optimized
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    88895-plasmid-map-sequence-id-451851

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  14. TFORF2775

    Plasmid
    #144234
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    KDM5C (KDM5C Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144234-plasmid-map-sequence-id-404121

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  16. pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP

    Plasmid
    #176279
    Purpose
    Viral vector for co-expression of non-functional Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.
    Depositor
    Martin Riccomagno
    Article
    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    Kir2.1MUT-P2A-EGFP (Kcnj2 Synthetic, Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    GYG to AAA (aa144-146)
    Promoter
    human Synapsin I
    Available Since
    Jan. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    176279-plasmid-map-sequence-id-350684

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  17. TFORF2778

    Plasmid
    #144157
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    KDM5C (KDM5C Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    144157-plasmid-map-sequence-id-396227

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  18. TFORF2774

    Plasmid
    #142160
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    KDM5C (KDM5C Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142160-plasmid-map-sequence-id-397717

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  19. FSF-GFP

    Plasmid
    #163658
    Purpose
    Expresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from TFR, TMD from ST6GAL1, and luminal domain from TFR in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and transferrin receptor (ST6GAL1, TFRC Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the cytosolic tail from TFR…
    Available Since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163658-plasmid-map-sequence-id-322789

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  20. JA412

    Plasmid
    #49935
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD KL-1 (KLF4 Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 4161 - 4180 of 4238 results