We narrowed to 11,655 results for: pcas

1. pCAG-CFP-SaCas9-HF (without sgRNA)

   Plasmid

   #134470

   Purpose

   Human expression vector for SaCas9-HF variant.

   Depositor

   Zongli Zheng

   Article

   Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p

   Insert

   Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
   

   Use

   CRISPR

   Tags

   SV40NLS-3xFLAG

   Expression

   Mammalian

   Mutation

   R245A, N413A, N419A, R654A

   Promoter

   CAG

   Available Since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAMBIA-FLS2prom::FLS2-3xmyc-GFP

   Plasmid

   #86157

   Purpose

   FLS2-GFP expresssion

   Depositor

   Silke Robatzek

   Article

   Robatzek et al Genes Dev. 2006 Mar 1;20(5):537-42.

   Insert

   FLS2 (FLS2 Mustard Weed)
   

   Tags

   3xmyc and GFP

   Expression

   Plant

   Available Since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-mCherry-FKBP-GEF(ARHGEF1)

   Plasmid

   #85152

   Purpose

   acute activation of Rho through rapamycin-induced translocation to membrane-localized FRB

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   Fusion of mCherry, FKBP, and the DH-domain of ARHGEF1 (amino acids 380-630) (ARHGEF1 Human)
   

   Tags

   mCherry-FKBP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGG>nls-hCas9-nls-GFP

   Plasmid

   #99141

   Purpose

   Cas9-GFP fusion protein under the regulation of chicken beta-actin promoter

   Depositor

   Marianne Bronner

   Article

   Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

   Insert

   Cas9-GFP
   

   Use

   CRISPR

   Tags

   GFP

   Promoter

   Chicken beta actin (CAG)

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2

   Plasmid

   #167295

   Purpose

   Plasmid encoding for 2 gRNAs targeting the human BAK gene and a CMV driven nuclease Cas9 followed by self-cleaving mCherry

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   BAK (BAK1 Human)
   

   Expression

   Mammalian

   Available Since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-mCherry-FKBP-GEF(TIAM1)

   Plasmid

   #85156

   Purpose

   acute activation of Rac through rapamycin-induced translocation to membrane-localized FRB

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   Fusion of mCherry, FKBP, and the DH-domain of human TIAM1 (amino acids 1012-1250)
   

   Tags

   mCherry-FKBP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCag SE (Self-excising) FlpO-2A-Cre EV

   Plasmid

   #130986

   Purpose

   episomal expression of FlpO and Cre recombinases, self excising

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   FlpO-2A-Cre
   

   Use

   Cre/Lox and Unspecified; Episomal expression vect…

   Expression

   Mammalian

   Mutation

   protamine intron added to FlpO to prevent bacteri…

   Promoter

   CMV/Chick β-actin (CAG)

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2

   Plasmid

   #167296

   Purpose

   Plasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFP

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   BAX (BAX Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. PET-B-Sniper SpCas9-NLS-6xHis

   Plasmid

   #207377

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Sniper SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F539S, M763I, K890N, amino acids 1005-1013 replac…

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-FLAG-puro-PTPIP51(1-470)

    Plasmid

    #170536

    Purpose

    Expresses PTPIP51(1-470) in human HeLa cells after transfection

    Depositor

    Byung Il Lee

    Article

    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

    Insert

    Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
    

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

11. pcs2+spCas9 ABE-Umax-nanos

    Plasmid

    #222142

    Purpose

    Adenine base editor with modified TadA variant for germline A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9 with nanos UTR
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Promoter

    SP6

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS SARS-CoV-2 D614G Spike

    Plasmid

    #185692

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 D614G Spike

    Depositor

    Marceline Côté

    Article

    Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

    Insert

    SARS-CoV-2 D614G Spike (S )
    

    Expression

    Mammalian

    Mutation

    Contains the following mutation: D614G

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available Since

    June 8, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. R26-pCAG-Ceru-10unit-BGHpA

    Plasmid

    #158387

    Purpose

    10-unit intMEMOIR recording array for mammalian cells

    Depositor

    Michael Elowitz

    Article

    Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

    Insert

    intMEMOIR 10-unit array
    

    Expression

    Mammalian

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCA12-pHR-SFFV-BFP-B23

    Plasmid

    #199443

    Purpose

    Expression of BFP-B23 in mammalian cells

    Depositor

    Baohui Chen

    Article

    Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

    Insert

    B23 (NPM1 Human)
    

    Use

    Lentiviral

    Tags

    TagBFP

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOTTC1080 - prRosa26v2 LSL FLAG-SpCas9

    Plasmid

    #113163

    Purpose

    A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKRG3-mU6-PUb-hSpCas9-pAc

    Plasmid

    #162163

    Purpose

    Expresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    hSpCas9
    sgRNA
    

    Use

    CRISPR

    Tags

    T2A-pAc

    Expression

    Insect

    Promoter

    Ae. aegypti PUb and Ae. aegypti U6

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKS004 - pCAGGS-3XFLAG-CTCF-eGFP

    Plasmid

    #156438

    Purpose

    For transient expression of mouse CTCF tagged with eGFP

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse CTCF (Ctcf Mouse)
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC CBA-SpCas9.EF1a-BFP.sgLMNA

    Plasmid

    #98971

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNA and spCas9
    

    Expression

    Mammalian

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-SpyTag-Flag-SRSF3-V5-mCherry

    Plasmid

    #235091

    Purpose

    SRSF3 mCherry fusion protein without MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-SpyTag-Flag-SRSF3-V5-mCherry (SRSF3 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-SpyTag-Flag-SRSF1-V5-mCherry

    Plasmid

    #235089

    Purpose

    SRSF1 mCherry fusion protein without MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-SpyTag-Flag-SRSF1-V5-mCherry (SRSF1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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