We narrowed to 16,621 results for: puromycin

1. hDNMT3B_KO_puro

   Plasmid

   #234998

   Purpose

   Stable knockout of DNMT3B function in mammalian cells

   Depositor

   Samrat Roy Choudhury

   Article

   Choudhury et al Genes & Diseases. 2025 Sep 6; 101843. do

   Insert

   hCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   July 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX459_LIG4_Exon3

   Plasmid

   #225363

   Purpose

   pX459 plasmid encoding the sgRNA protospacer sequence “5’-GCATAATGTCACTACAGATC-3’ to target the LIG4 gene.

   Depositor

   Sujatha Jagannathan

   Article

   Cortazar et al Methods Mol Biol. 2025;2863:397-417. doi

   Insert

   LIG4 (LIG4 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFUW PRKN-P2A-PuroR

   Plasmid

   #239571

   Purpose

   Overexpression of human PARK2

   Depositor

   Tim Ahfeldt, Joel Blanchard

   Article

   et al iScience 28 (2025)

   Insert

   PRKN (PRKN Human)
   

   Use

   Lentiviral

   Promoter

   UbC

   Available Since

   June 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSG297-(Sp-gRNA)-(Sa-gRNA)-(PuroR_T2A_BFP(C>T screening))

   Plasmid

   #239448

   Purpose

   Orthogonal dual Cas9 gRNA and BFP reporter lentivirus cassette plasmid. For use with S. pyogenes and S. aureus gRNAs and includes a BFP reporter which reports on C-to-T editing.

   Depositor

   Alexis Komor

   Inserts

   S.p. gRNA backbone
   S.a. gRNA backbone
   PuroR-T2A-BFP(C>T_screening)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   BFP: T65S, S72S, V145F, K206K, H231L

   Promoter

   EF1alpha, U6, and mU6

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR Ef1a_LaG16-synNotch_tTA-PGK_Puro

   Plasmid

   #223536

   Purpose

   Anti-GFP LaG16 nanobody-based synNotch; puro-selectable

   Depositor

   Jonathan Brunger

   Article

   Walton et al Osteoarthritis Cartilage. 2025 Feb;33(2)

   Insert

   LaG16_synNotch_tTA
   

   Use

   Lentiviral

   Tags

   c-myc tag

   Expression

   Mammalian

   Available Since

   June 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRCA595 - pBA904 Puro-T2A-mCherry

   Plasmid

   #238171

   Purpose

   Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

   Depositor

   Thomas Norman

   Article

   Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

   Insert

   Non-targeting sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRCA594 - pBA904 Puro-T2A-BFP

   Plasmid

   #238170

   Purpose

   Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

   Depositor

   Thomas Norman

   Article

   Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

   Insert

   Non-targeting sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   mTagBFP2

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-TMEM9-EGFP

   Plasmid

   #225515

   Purpose

   Lentiviral TMEM9-EGFP expression vector

   Depositor

   Wade Harper

   Article

   Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

   Insert

   TMEM9 (TMEM9 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLJC2-NADK (D184A)-3xFLAG

   Plasmid

   #233889

   Purpose

   lentiviral overexpression vector for NADK (D184A)

   Depositor

   Jason Cantor

   Article

   Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

   Insert

   NADK (NADK Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D184A

   Promoter

   CMV

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. MCUb-mVenus-pLJM

    Plasmid

    #236789

    Purpose

    MCUB tagged with mVenus for FRET / MW change for stoichiometry

    Depositor

    Dipayan Chaudhuri

    Article

    Rai et al J Cell Physiol. 2025 Apr;240(4):e70033.

    Insert

    MCUB (MCUB Human)
    

    Use

    Lentiviral

    Tags

    mVenus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-TetOne-Puro-LbNOX-Flag

    Plasmid

    #234984

    Purpose

    Expresses LbNOX in human cell lines

    Depositor

    Denis Titov

    Article

    Choe et al Cell Chem Biol. 2025 Apr 17;32(4):620-63

    Insert

    LbNOX
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. EW172 CMV-TO-UTRN-14ZF-VP64 (FLP-IN)

    Plasmid

    #236160

    Purpose

    Plasmid encoding the UTRN-14 zinc finger array attached by a GS linker to the VP64 transcriptional activation domain, under control of CMV promoter with two TetR binding sites

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    UTRN-14ZF-VP64
    

    Expression

    Mammalian

    Promoter

    CMV promoter with two TetR binding sites

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. EW215 PGK-ZNF35(5-8)-deImmunLink-NZF(FLP-IN)

    Plasmid

    #236130

    Purpose

    Plasmid encoding the ZNF35-derived portion of the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoter

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    ZNF35(5-8)-deImmunLink-NZF
    

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. JPZF4 CMV-TO-SCN1A-383-NZF

    Plasmid

    #236165

    Purpose

    Plasmid encoding the SCN1A-383 zinc finger array attached by a GS linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sites

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    SCN1A-383-NZF
    

    Expression

    Mammalian

    Promoter

    CMV promoter with two TetR binding sites

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. JPZF5 CMV-TO-SCN1A-472-NZF

    Plasmid

    #236166

    Purpose

    Plasmid encoding the SCN1A-472 zinc finger array attached by a GS linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sites

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    SCN1A-472-NZF
    

    Expression

    Mammalian

    Promoter

    CMV promoter with two TetR binding sites

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. EW269 CMV-TO-Gal4-NZF[GS Linkers] (FLP-IN)

    Plasmid

    #236122

    Purpose

    Plasmid encoding Gal4-NZF transcription factor with GS linkers between the component domains of NZF, under control of CMV promoter with two TetR binding sites

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    Gal4-NZF (GS Linkers)
    

    Expression

    Mammalian

    Promoter

    CMV promoter with two TetR binding sites

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. EW152 CMV-TO-Gal4-NZF[Mutated Linkers] (FLP-IN)

    Plasmid

    #236123

    Purpose

    Plasmid encoding Gal4-NZF transcription factor with deimmunized linkers between the component domains of NZF, under control of CMV promoter with two TetR binding sites

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    Gal4-NZF (mutated linkers)
    

    Expression

    Mammalian

    Promoter

    CMV promoter with two TetR binding sites

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. EW216 PGK-ZNF250(1-4)-deImmunLink-NZF(FLP-IN)

    Plasmid

    #236131

    Purpose

    Plasmid encoding the ZNF250-derived portion of the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoter

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    ZNF250(1-4)-deImmunLink-NZF
    

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. JPZF9 CMV-TO-SCN1A-371-NZF

    Plasmid

    #236170

    Purpose

    Plasmid encoding the SCN1A-371 zinc finger array attached by a GS linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sites

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    SCN1A-371-NZF
    

    Expression

    Mammalian

    Promoter

    CMV promoter with two TetR binding sites

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EW490 CMV-TO-UTRN-14ZF-realdeImmLink-NZF (FLP-IN)

    Plasmid

    #236149

    Purpose

    Plasmid encoding the UTRN-14 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sites

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    UTRN-14ZF-deImmLink-NZF
    

    Expression

    Mammalian

    Promoter

    CMV promoter with two TetR binding sites

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge