We narrowed to 7,815 results for: Lif

1. pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)

   Plasmid

   #86424

   Purpose

   PCR tagging module with yeast codon optimized mTurquoise2

   Depositor

   Sue Jaspersen

   Article

   Burns et al Elife. 2015 Sep 15;4. doi: 10.7554/eLife

   Insert

   mTurquoise2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac1-OSF-TRIM5alpha(human)

   Plasmid

   #79034

   Purpose

   Baculoviral entry vector to produce Bac-to-bac baculoviruses

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OSF-TRIM5alpha(human) (TRIM5 Human)
   

   Use

   Tags

   OneSTrEP-FLAG

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   March 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. GST-TPX2(1-43)

   Plasmid

   #69107

   Purpose

   For bacterial expression of human TPX2(1-43aa) tagged with GST.

   Depositor

   Stephen Royle

   Article

   Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

   Insert

   Targeting Protein for Xklp2 (TPX2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Amino acids 1-43 expressed.

   Promoter

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC57-Amp_CRISPIE donor B3

   Plasmid

   #172844

   Purpose

   CRISPIE donor B3 (Zhong et al, eLife 2021), mTurquoise2 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   CRISPIE designer exon (phase 0-0) encoding mTurquoise2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC57-Amp_CRISPIE donor B4

   Plasmid

   #172845

   Purpose

   CRISPIE donor B4 (Zhong et al, eLife 2021), mNeonGreen translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   CRISPIE designer exon (phase 0-0) encoding mNeonGreen
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3xNLS-SGFP2(T65G)-T2A-SGFP2-3xNES

   Plasmid

   #60492

   Purpose

   Co-expresses two green fluorescent protein variants, a short fluorescence lifetime GFP variant in the nucleus and a GFP variant with a long lifetime in the cytoplasm

   Depositor

   Dorus Gadella

   Article

   Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.

   Inserts

   3xnls-SGFP2(T65G)
   SGFP2-3xNES
   

   Use

   Tags

   SGFP2 and SGFP2(T65G)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-syn-IRES-hrGFP

   Plasmid

   #107549

   Purpose

   high transduction efficiency AAV-mediated synapsin promoter-dependent expression IRES-hrGFP

   Depositor

   Hélène Marie

   Article

   Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA 5/FR/TO-Ubl4A

   Plasmid

   #61810

   Purpose

   express C-terminal Ubl4A, for making the stable cell line

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   UBL4A (UBL4A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pRKB134_GI-DUX4-intron2(-)

   Plasmid

   #83968

   Purpose

   to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

   Depositor

   Robert Bradley

   Article

   Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

   Insert

   DUX4-3'UTR-intron2(-) (DUX4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouseCMV

   Available since

   Jan. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pRKB133_GI-DUX4-intron2(+)

    Plasmid

    #83967

    Purpose

    to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

    Depositor

    Robert Bradley

    Article

    Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

    Insert

    DUX4-3'UTR-intron2(+) (DUX4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouseCMV

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pFastBac1-OSF-TRIM5alpha(chimpanzee)

    Plasmid

    #79032

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(chimpanzee)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCZGY1584 Pcol-19::ebp-2::mGFP::unc-54 3'UTR

    Plasmid

    #129774

    Purpose

    Marker of microtubule dynamics in C. elegans hypodermis

    Depositor

    Andrew Chisholm

    Article

    Chuang et al Elife. 2016 Sep 23;5. doi: 10.7554/eLife

    Inserts

    Pcol-19
    ebp-2 (ebp-2 Nematode)
    unc-54 3'UTR
    

    Use

    Synthetic Biology; Gateway

    Tags

    Synthetic Intron and mGFP

    Expression

    Worm

    Mutation

    Promoter

    Pcol-19

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. HS-DN-Ect2

    Plasmid

    #105956

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

    Insert

    Ect2 (ECT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. px330-gp78

    Plasmid

    #61833

    Purpose

    to make gp78 Knockout cell line

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    gp78 (AMFR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ Lox 5'UTR

    Plasmid

    #67932

    Purpose

    Translational reporter - Lox 5'UTR

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

    Insert

    sfGFP-NLS-P2A-NLS-mCherry (LOX Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUASTLOTattB_mCherry::Nrv1::vhhGFP4

    Plasmid

    #163926

    Purpose

    Expression of GrabFP-B-intracellular under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUASTLOTattB_vhhGFP4::Vkg::mCherry

    Plasmid

    #163929

    Purpose

    Expression of GrabFP-ECM under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 nanobody with Viking (Collagen IV) protein and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Mutation

    G1767D

    Promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJW3.42

    Plasmid

    #62866

    Purpose

    Yeast two-hybrid bait vector expressing LexA-PPTR-1 fusion

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    PPTR-1/pLexA-N bait
    

    Use

    Two-hybrid bait vector

    Tags

    LexA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA 5/FR/TO-Ubl4A K48R

    Plasmid

    #61811

    Purpose

    express C-terminal Ubl4A K48R, for making the stable cell line

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    UBL4A (UBL4A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K48R

    Promoter

    CMV

    Available since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

20. VEE-mScarlet3-IRES-EGFP-IRES-moxBFP

    Plasmid

    #242407

    Purpose

    Native (conventional) self-amplifying RNA (saRNA) control construct expresses moxBFP as visual marker of IRES functionality

    Depositor

    Ewan St. John Smith

    Article

    Lim et al bioRxiv 2024.09.24.614636

    Inserts

    mScarlet3
    IRES-EGFP
    IRES-moxBFP
    

    Use

    Self-amplifying rna

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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