We narrowed to 18,031 results for: Mut
-
Plasmid#220278PurposeConstitutive expression of a chimeric LacI:ScrR transcription repressorDepositorInsertLLhScrR
UseTagsExpressionBacterialMutationV52I/Q62S/G120SPromoterAvailable SinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQdCas12a.luxR(mut)-sggfp(B)
Plasmid#236041PurposeThe plasmid pQdCas12a.luxR(mut)-sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.DepositorInsertquorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
UseCRISPRTagsExpressionMutationPromoterquorum sensing promoterAvailable SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQdCas12a.luxR(mut)-sggfp(A)
Plasmid#236039PurposeThe plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.DepositorInsertquorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
UseCRISPRTagsExpressionMutationPromoterquorum sensing promoterAvailable SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3_5UTR_Myc_FLuc Compensatory Mutant
Plasmid#229504PurposeMyc 5' untranslated region firefly luciferase reporter with compensatory mutations in the region bound by RBM42DepositorInsertMyc 5'UTR_CompMutant
UseTagsExpressionMammalianMutationMutations in bp 363-394PromoterSV40Available SinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
PGL4.11-COL1A1double mutants
Plasmid#230918PurposeDouble mutation of TEAD and SMAD binding elements in COL1A1 promoter; check the promoter activity.DepositorInsertHuman CLO1A1 promoter including SMAD and TEAD mutant elements (COL1A1 Human)
UseLuciferaseTagsLuciferase-luc2pExpressionBacterial and MammalianMutationCOL1A1 promoter with mutant SMAD and TEAD binding…PromoterCOL1A1 with mutant SMAD and TEAD elementsAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-BRAF_WT&Mut-SM
Plasmid#215916PurposeThis plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of siBRAF_WT&Mut (BRAF Human)
UseTagsExpressionMammalianMutationPromoterAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-BRAF_WT&Mut-CM
Plasmid#215920PurposeThis plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.DepositorInsertone complete-matched (CM) sequence of siBRAF_WT&Mut (BRAF Human)
UseTagsExpressionMammalianMutationPromoterAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTFG002-ACE2(Mut)-HA
Plasmid#191570PurposeACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) to generate ACE2(Mut)-expressing cells. Codon-optimized for human expression. Insert contains C-terminal HA Tag.DepositorInsertAngiotensin Converting Enzyme II (ACE2 Human)
UseLentiviral and RNAiTagsHA tagExpressionMammalianMutationCodon-optimized for human expression. Also contai…PromoterCMVAvailable SinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-TAF5-FL_WobbleMut
Plasmid#209053PurposeGateway-compatible entry vector, with insert of the full length TAF5 gene mutated to be resistant to TAF5 siRNADepositorInsertTAF5 full length
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-TAF5-∆ex8_WobbleMut
Plasmid#209054PurposeGateway-compatible entry vector, with insert of the TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNADepositorInsertTAF5 deltaexon-8
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGB-V5-TurboID-MUTT2A-C
Plasmid#218897Purposegolden gate cloningDepositorInsertV5-TurboID-MUTT2A
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
B2M-SDMutation-EhT
Plasmid#215546PurposeDonor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-outDepositorInsertB2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived hygromycin-NLS-tdTomato selection cassette (B2M Human)
UseCRISPRTagsExpressionMutationThe second base of the first intron of B2M (From …PromoterAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
B2M-SDMutation-EPG
Plasmid#215545PurposeDonor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-outDepositorInsertB2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived puromycin-GFP selection cassette (B2M Human)
UseCRISPRTagsExpressionMutationThe second base of the first intron of B2M (From …PromoterAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
B2M-SDMutation-gRNA
Plasmid#215547PurposegRNA targeting B2M to introduce splice donor mutation on the first intron of the locusDepositorAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFlare9A-Clip2E9 Mutant
Plasmid#209575Purposeminigene construct for Clip2 E9 alternative splicing, potential PTBP2 binding sites are deletedDepositorInsertClip2 (Clip2 Mouse)
UseTagsExpressionMammalianMutationACGCGTTTCTGAATTCTTCTAACTGCCCTCAAATGCACGGTGGCATGTG…PromoterCMVAvailable SinceMarch 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Caspase-11_humanized-mutant
Plasmid#214315PurposeProtein overexpression in bacteriaDepositorInsertCASP4 (Casp4 Mouse)
UseTagsHis-TEVExpressionBacterialMutationN152K_KLS288YKT_KASIHS348TPRAKAPromoterT7Available SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAB-HDAC5(mut-H-Y)
Plasmid#114398PurposeFor PYL-HDAC5 H1006Y expressionDepositorInsertPYL-HDAC5 H1006Y
UseTagsExpressionMammalianMutationadditional D593E compared to NCBI ref NP_005465.2PromoterCMVAvailable SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_r5-HTmut
Plasmid#208714PurposeExpresses the red 5-HT sensor GRAB_r5-HTmut in mammalian cellsDepositorInsertRed fluorescent 5-HT sensor GRAB_r5-HTmut
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_g5-HT3.0mut
Plasmid#208712PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0mut in mammalian cellsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0mut
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAB-HDAC5(mut-H-A)
Plasmid#114397PurposeFor PYL-HDAC5 H1006A expressionDepositorInsertPYL-HDAC5 H1006A
UseTags3xFlag-NLS (internal)ExpressionMammalianMutationD593E compared to NCBI ref NP_005465.2PromoterCMVAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only