We narrowed to 9,373 results for: Pol;
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Plasmid#11424DepositorInsertaP2 promoter (Fabp4 Mouse)
ExpressionBacterialAvailable SinceApril 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
Polymerase expression - pCMV-Phi29 (LM2708)
Plasmid#208965PurposeUnfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.DepositorInsertPhi29-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationPhi29(-exo;D169A)PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AP1muA-Halo-ALFA-polyA-G418 HR
Plasmid#229681PurposeHomology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.DepositorAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA
Plasmid#105648PurposeExpression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeksDepositorInsertTol2-Isl2b:GFP:polyA (isl2b Zebrafish)
ExpressionMammalianAvailable SinceFeb. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-SV40 polyA signal
Plasmid#48627PurposeContributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.DepositorInsertSV40 early polyadenylation signal cassette
UseGateway entry vectorPromoternoneAvailable SinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
single polypeptide FPX biosensor for caspase-3
Plasmid#60883PurposeFPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs).DepositorInsertsingle polypeptide FPX biosensor for caspase-3
TagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET empty polycistronic destination vector (2D)
Plasmid#29774DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-FHC-POLQ-DY2230AA
Plasmid#64878Purposelentiviral expression of human POLQ -DY2230AA mutant (a polymerase domain mutant)DepositorInsertPOLQ (POLQ Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationD2330A,Y2331A, in the DNA polymerase domain (POL)…PromoterEF1alphaAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
PL452-hGH-polyA-PGK-neoR
Plasmid#191681PurposeDonor plasmid backbone PGK-neoRDepositorTypeEmpty backboneExpressionMammalianMutationWTAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-tdTomato-polyA
Plasmid#174022PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInserttdTomato
UseZebrafish knock-in taggingAvailable SinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET empty polycistronic destination vector (2E)
Plasmid#29775DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLV-CMV-EGFP-PolB-Hygro
Plasmid#176056PurposeEGFP fused to the N-terminus of PolB & a hygromycin resistance cassetteDepositorAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-POLR2A-CTD-mCherry
Plasmid#185011PurposeBacterial Expression of 6xHis C-terminal domain of POLR2A mCherry Fusion ProteinDepositorInsertPOLR2A (Polr2a Mouse)
ExpressionBacterialAvailable SinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
LncRNA overexpression EF1α-BbsI-SV40polyA_puroR
Plasmid#219826PurposeOverexpression vector for non-coding RNAs driven by EF1α promoter with puromycin resistance. Cloning using BbsI.DepositorTypeEmpty backboneExpressionMammalianPromoterEF1aAvailable SinceJuly 29, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
Polymerase expression - pCMV-T7-EcKlenow (LM2692)
Plasmid#208963PurposeUnfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.DepositorInsertEcKlenow-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationEcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDH3pro-rtTA-tetO7pro-T7pol-pRS413
Plasmid#239293PurposeExpresses the reverse tetracycline activator (rtTA) and T7 polymerase; Plasmid #1 of the tet-on overexpression systemDepositorInsertsT7 polymerase with NLS and P278L
reverse tetracycline transactivator (rtTA)
ExpressionYeastMutationContains M2-SE-G72P mutations, described in Addge…PromoterTDH3 and tetracycline-responsive element (TRE) an…Available SinceJune 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR_5xUAS-BFP-WPRE_PGK-HygR-bGHpolyA
Plasmid#216665PurposeExpresses Gal4 inducible BFP, with hygromycin resistanceDepositorInsertsBFP
HygromycinR
UseLentiviralAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Ran-T24N-mRFP1-polyA
Plasmid#104561PurposeExpress Ran T24N mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-flag-hKDM4A(H188A)-polyA
Plasmid#101052Purposein vitro transcription (T7 promoter)DepositorAvailable SinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Ran-Q69L-mRFP1-polyA
Plasmid#104560PurposeExpress Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only