Addgene

We narrowed to 7,975 results for: abo

Showing: 401 - 420 of 7975 results

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pCTD26-S>A10-17

Plasmid

#112826

Purpose

pSMF2 with all S to A mutations in only repeats 10-17.

Depositor

Stephen Fuchs

Article

Babokhov et al G3 (Bethesda). 2018 May 4;8(5):1593-1601

Insert

RPB1 (RPO21 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

pSMF2 with all S to A mutations in only repeats 1…

Promoter

Native promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCTD26-S>A2-9

Plasmid

#112825

Purpose

pSMF2 with all S to A mutations in only repeats 2-9.

Depositor

Stephen Fuchs

Article

Babokhov et al G3 (Bethesda). 2018 May 4;8(5):1593-1601

Insert

RPB1 (RPO21 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

pSMF2 with all S to A mutations in only repeats 2…

Promoter

Native promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCTD26-S>A18-25

Plasmid

#112827

Purpose

pSMF2 with all S to A mutations in only repeats 18-25.

Depositor

Stephen Fuchs

Article

Babokhov et al G3 (Bethesda). 2018 May 4;8(5):1593-1601

Insert

RPB1 (RPO21 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

pSMF2 with all S to A mutations in only repeats 1…

Promoter

Native promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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B3rec.zf1

Plasmid

#108354

Purpose

Zebrafish codon optimized B3 recombinase that acts on blown-out sites

Depositor

Harold Burgess

Article

Tabor et al Curr Biol. 2018 Aug 20;28(16):2527-2535.

Insert

B3 recombinase

Use

Synthetic Biology

Tags

Expression

Mutation

Zebrafish codon optimized

Promoter

lac

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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LifeAct-mCherry

Plasmid

#193723

Purpose

Labeling microtubules in living mammalian cells

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

LifeAct-mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

mCherry: Met1 Deleted

Promoter

Chicken beta-actin

Available since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pBetaActin-dCer3-C2-FRB

Plasmid

#191327

Purpose

Expresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-C2-dCer3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FRB-dCer3-CAAX

Plasmid

#191326

Purpose

Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-dCer-CAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-mEos3.2

Plasmid

#191320

Purpose

Expresses fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mEos3.2 (Tubb2a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-Dronpa

Plasmid

#191321

Purpose

Expresses fuorescently labeled beta tubulin to allow photoactivation of microtubule patches with very little UV light

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-Dronpa (Tubb2a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pKB236.1

Plasmid

#170028

Purpose

Encodes LacI-controlled expression of surface-displayed TCP fragment lacking C-terminal alpha-helix sequence and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
TCP Δ C-terminal alpha-helix

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Mutation

Promoter

Ptac and PvirK

Available since

Availability

Academic Institutions and Nonprofits only

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pSR43.6r

Plasmid

#63197

Purpose

Expresses CcaS, Ho1, and PcyA constitutively

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

CcaS
Ho1
PcyA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pSR58.6

Plasmid

#63176

Purpose

Expresses CcaR constitutively and sfGFP under the PcpcG2-172 promoter

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

CcaR
sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pSR33.4r

Plasmid

#63198

Purpose

Expresses Cph8, Ho1, and PcyA constitutively

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

Cph8
Ho1
PcyA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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pBL4

Plasmid

#50548

Purpose

Expresses CcaS and CcaR constitutively, and sfGFP/TetR under the PcpcG2 promoter

Depositor

Jeffrey Tabor

Article

Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

Inserts

CcaS
CcaR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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pBAMD1-4

Plasmid

#61565

Purpose

Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pBAMD1-2

Plasmid

#61564

Purpose

Standardized mini-Tn5 delivery plasmid, kanamycin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pKD238-1a

Plasmid

#90958

Purpose

Expresses S. baltica TtrS constitutively

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Insert

Tetrathionate sensor kinase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Bba_J23109

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pBAMD1-6

Plasmid

#61566

Purpose

Standardized mini-Tn5 delivery plasmid, gentamicin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pPR220.08

Plasmid

#78553

Purpose

Plasmid constitutively expressing phycocyanobilin (PCB) biosynthesis genes and UirS sensor kinase gene

Depositor

Jeffrey Tabor

Article

Ramakrishnan et al ACS Synth Biol. 2016 Apr 27.

Insert

uirS

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

J23108

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pNO286-3

Plasmid

#107746

Purpose

Key genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)

Depositor

Jeffrey Tabor

Article

Ong et al Chembiochem. 2018 Feb 8. doi: 10.1002/cb

Inserts

CcaS
Ho1
PcyA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Deleted AA 223 and 512

Promoter

J23106 and J23108

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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