We narrowed to 4,204 results for: dcas9

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pHR-TRE3G-dCas9-HaloTag

Plasmid

#169448

Purpose

expression of dCas9-HaloTag

Depositor

Stanley Qi

Publication

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

dCas9-HaloTag

Use

Lentiviral

Expression

Mammalian

Available since

June 13, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTPGI_dCas9_VP64

Plasmid

#49013

Purpose

encodes yeast-optimized dCas9_VP64 synthetic transcription factor

Depositor

Timothy Lu

Publication

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

dCas9_VP64 (codon-optimized for expression in S. cerevisiae)

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Yeast

Promoter

pTPGI (galactose+aTc inducible)

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pXPR_123

Plasmid

#96919

Purpose

for CRISPRi, lentiviral expression of dCas9-p300 2A BlastR.

Depositor

John Doench, David Root

Publication

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Insert

dCas9

Use

Lentiviral

Tags

p300

Expression

Mammalian

Mutation

dCas9

Promoter

EF1a

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAW90.dCas9-YY1

Plasmid

#104373

Purpose

Expresses dCas9-YY1

Depositor

Richard Young

Publication

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-YY1

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTPGI_dCas9_VP64

Plasmid

#49013

Purpose

encodes yeast-optimized dCas9_VP64 synthetic transcription factor

Depositor

Timothy Lu

Publication

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

dCas9_VP64 (codon-optimized for expression in S. cerevisiae)

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Yeast

Promoter

pTPGI (galactose+aTc inducible)

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pEF1a-BirA-V5-neo

Plasmid

#100548

Purpose

Expresses C-terminal V5-tagged BirA biotin ligase

Depositor

Jian Xu

Publication

Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

C-terminal V5-tagged BirA

Tags

V5, His

Expression

Mammalian

Promoter

Human EF1a

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9

Plasmid

#84243

Purpose

Expresses ABA-inducible VPR-Sa dCas9

Depositor

Stanley Qi

Publication

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

ABI-tagBFP-Sa dCas9
PYL1-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

ABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …

Expression

Mammalian

Mutation

D10A, N580A and Synonymous mutation in PYL1 to re…

Promoter

CAG and PGK

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9

Plasmid

#84243

Purpose

Expresses ABA-inducible VPR-Sa dCas9

Depositor

Stanley Qi

Publication

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

ABI-tagBFP-Sa dCas9
PYL1-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

ABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …

Expression

Mammalian

Mutation

D10A, N580A and Synonymous mutation in PYL1 to re…

Promoter

CAG and PGK

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pXPR_123

Plasmid

#96919

Purpose

for CRISPRi, lentiviral expression of dCas9-p300 2A BlastR.

Depositor

John Doench, David Root

Publication

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Insert

dCas9

Use

Lentiviral

Tags

p300

Expression

Mammalian

Mutation

dCas9

Promoter

EF1a

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEF1a-BirA-V5-neo

Plasmid

#100548

Purpose

Expresses C-terminal V5-tagged BirA biotin ligase

Depositor

Jian Xu

Publication

Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

C-terminal V5-tagged BirA

Tags

V5, His

Expression

Mammalian

Promoter

Human EF1a

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pdestMB14_sur5p-dCas9-VP64

Plasmid

#177788

Purpose

Overexpression of dCas9-VP64 in C. elegans

Depositor

Michael Ristow

Publication

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

dCas9-VP64

Expression

Worm

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

Plasmid

#84244

Purpose

Expresses GA-inducible VPR-Sa dCas9

Depositor

Stanley Qi

Publication

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

GAI-tagBFP-Sa dCas9
GID1-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …

Expression

Mammalian

Mutation

D10A, N580A and Synonymous mutation in PYL1 to re…

Promoter

CAG and PGK

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

Plasmid

#84244

Purpose

Expresses GA-inducible VPR-Sa dCas9

Depositor

Stanley Qi

Publication

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

GAI-tagBFP-Sa dCas9
GID1-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …

Expression

Mammalian

Mutation

D10A, N580A and Synonymous mutation in PYL1 to re…

Promoter

CAG and PGK

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Lenti-dCas9-TurboID-blast

Plasmid

#222599

Purpose

Express fussed dCas9-TurboID for proximity labeling

Depositor

Yarui Diao

Publication

Sun et al Nat Genet. 2023 Aug;55(8):1324-1335. doi

Insert

dCas9-TurboID

Use

Lentiviral

Promoter

EF-1α

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pGH335_MS2-AID*Δ-Hygro

Plasmid

#85406

Purpose

lentiviral expression vector for MS2-AID*Δ fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Publication

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AID*Δ and Hygromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

Plasmid

#85756

Purpose

All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).

Depositor

Lawrence Stanton

Publication

Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Csy4-T2A-SpRFN-P2A-GFP
gRNA

Use

CRISPR

Tags

Csy4, Csy4 recognition sequence, FokI fusion via …

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CMV and U6

Available since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pdestMB14_sur5p-dCas9-VP64

Plasmid

#177788

Purpose

Overexpression of dCas9-VP64 in C. elegans

Depositor

Michael Ristow

Publication

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

dCas9-VP64

Expression

Worm

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

Plasmid

#85756

Purpose

All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).

Depositor

Lawrence Stanton

Publication

Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Csy4-T2A-SpRFN-P2A-GFP
gRNA

Use

CRISPR

Tags

Csy4, Csy4 recognition sequence, FokI fusion via …

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CMV and U6

Available since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b-BE1

Plasmid

#73018

Purpose

Expresses BE1 with N-terminal His6 tag in E. Coli

Depositor

David Liu

Publication

Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

Insert

BE1

Tags

His6 tag

Expression

Bacterial

Promoter

T7

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGH224_sgRNA_2xMS2_Puro

Plasmid

#85413

Purpose

hU6 driven sgRNA vector with 2xMS2 vectors with puro selectable marker

Depositor

Michael Bassik

Publication

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

puromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge