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Plasmid#164218PurposeExpresses mEmerald tagged CAPRIN1 under EF-1a promoterDepositorInsertCAPRIN1 (CAPRIN1 Human)
UseLentiviralTagsmEmeraldExpressionMutationPromoterEF-1aAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EF1aL-TagBFP-W
Plasmid#126687PurposeConstitutive, ubiquitous blue fluorescence protein (BFP) expression.DepositorInsertTagBFP
UseLentiviralTagsExpressionMutationPromoterEf1aLAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-mSTING
Plasmid#229739PurposeExpresses mouse STING in mammalian cells. Stable expression with piggybac transposase.DepositorInsertSTING (Sting1 Mouse)
UseTagsNoneExpressionMammalianMutationPromoterEF1AAvailable sinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G
Plasmid#194458PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-CD19 CAR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-HaloSFPQ
Plasmid#166948PurposeLentiviral plasmid expressing Halo-tagged SFPQ protein from the EF1a promoterDepositorInsertSFPQ (SFPQ Human)
UseLentiviralTagsHaloExpressionMutationPromotereF1aAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-IRES-Neo
Plasmid#28019DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-Ef1a-mCh
Plasmid#31847PurposeCre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.DepositorTypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNA; Ef1alpha for mCherryAvailable sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#113341PurposeExpresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteinsDepositorInsertVP64-dCas9-VP64-T2A-MS2-p65-HSF1
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mito-dsRED2
Plasmid#174541Purposemitochondria targeted dsRED expressed off of EF1a promoterDepositorInsertMito
UseLentiviralTagsdsREDExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS598 EF1alpha-EGFPcre
Plasmid#11923PurposeExpresses EGFP-Cre fusion under the control of the EF1alpha promoter; EGFP is human codon-optimized.DepositorInsertEGFP-cre (cre bacteriophage P1)
UseCre/LoxTagsEGFPExpressionMammalianMutationPromoterEF1alphaAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH c-Met
Plasmid#86498PurposeMammalian expression of human c-MetDepositorInsertMet (MET Human)
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMV1 pEF1a-HGF
Plasmid#188748PurposeVector constitutively expressing HGFDepositorInsertHGF (HGF Human)
UseTagsExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-mCherry
Plasmid#114470PurposemCherry ControlDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertmCherry
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
EF1a- gfp-2a-puro
Plasmid#129443Purposeexpression of GFP and Puro RDepositorInsertgfp-puro
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#113343PurposeExpresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteinsDepositorInsertp300core-dCas9-VP64-T2A-MS2-p65-HSF1
UsePiggybacTagsExpressionMutationPromoterAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lv EF1a dCas9-HA 2A Blast
Plasmid#102812PurposeLentiviral plasmid from FIRE-Cas9 system to express dCas9-HA fusion proteinDepositorInsertdCas9-HA
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A (dCas9)PromoterEF1aAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti_Ef1a:PE2-P2A-GFP
Plasmid#184445PurposeSingle EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and Ef1AAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-Cas9Bsd-W
Plasmid#68343PurposeLentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminusDepositorInsertEF1a-Cas9 cassette, WPRE
UseCRISPR and LentiviralTagsCas9 fused with Flag-tag and a NLS at the N termiā¦ExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PEW-pEF1A-inactEGFP
Plasmid#212009PurposeCleavable backbone for constitutive expression of TCR alpha and beta chains through lentiviral transductionDepositorInsertInactivated eGFP
UseLentiviralTagsExpressionMammalianMutationPromoterpEF1aAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-hSNCA
Plasmid#170445PurposeLentiviral vector expressing human SNCA, under control of EF1alpha promoter.DepositorInserthuman SNCA (SNCA Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only