We narrowed to 15,096 results for: ef1

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LV_EF1a_SV40_Large_T_Antigen-P2A-Hygro_Barcode

Plasmid

#170255

Purpose

Barcoded lentiviral vector to express SV40 Large T Antigen in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

SV40 Large T Antigen

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pT3-EF1A-mTert

Plasmid

#162555

Purpose

Overexpression of Tert

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

Overexpression of Tert (Tert Mouse)

Expression

Mammalian

Mutation

None

Promoter

EF1A

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a-TET1-dCas9

Plasmid

#235593

Purpose

Tet1CD fused to N-terminus of dCas9

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

TET1-dCas9 (TET1 Human, SpCas9 is from Streptococcus pyogenes)

Use

CRISPR

Tags

3xNLS, TET1 CD, and tagBFP

Expression

Mammalian

Promoter

EF1a

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-EF1Alpha-Tet-on 3G

Plasmid

#118592

Purpose

Expresses Tet-on 3G from the EF1Alpha promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

Tet-on 3G

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pT3-EF1a-YapS127A

Plasmid

#46049

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

Flag tag

Expression

Mammalian

Mutation

S127A

Promoter

EF1a

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pT3-EF1aH NICD1

Plasmid

#86500

Purpose

Mammalian expression of Myc tagged NICD1

Depositor

Xin Chen

Article

Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

Insert

Notch1 (C-term) (Notch1 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

Cytoplasmic domain only (AA 1753-2531)

Promoter

EF1a

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pBS598 EF1alpha-EGFPcre

Plasmid

#11923

Purpose

Expresses EGFP-Cre fusion under the control of the EF1alpha promoter; EGFP is human codon-optimized.

Depositor

Brian Sauer

Article

Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

Insert

EGFP-cre (cre bacteriophage P1)

Use

Cre/Lox

Tags

EGFP

Expression

Mammalian

Promoter

EF1alpha

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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EF1a-dCasRx-RBM25

Plasmid

#221001

Purpose

Transient expression of dCasRx-RBM25. EF1a promoter.

Depositor

Benjamin Blencowe, Mikko Taipale

Article

Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

Insert

dCasRx-RBM25

Use

CRISPR

Tags

NLS and NLS-HA

Expression

Mammalian

Promoter

EF1a

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCVL.SFFV.d14GFP.EF1a.HA.NLS.Sce(opt).T2A.TagBFP

Plasmid

#32627

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Donor + Sce BFP

Use

Lentiviral

Available Since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G

Plasmid

#194458

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-CD19 CAR

Use

Lentiviral

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_CDK4-P2A-Hygro_Barcode

Plasmid

#170223

Purpose

Barcoded lentiviral vector to express CDK4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK4 (CDK4 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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p5E-EF1a/b-actin

Plasmid

#82583

Purpose

5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expression

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

EF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1aGFP2AKAT7(E508Q)-W

Plasmid

#159293

Purpose

Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, E508Q mutant) driven by human EF1a promoter

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

KAT7 E508Q

Use

Lentiviral

Expression

Mammalian

Mutation

CRISPR sites mutated (gRNA ID. 5 and A10) and E50…

Promoter

human EF1a promoter

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pT3-EF1a-c-Met

Plasmid

#31784

Depositor

Xin Chen

Article

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

Met (MET Human)

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113341

Purpose

Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

VP64-dCas9-VP64-T2A-MS2-p65-HSF1

Use

Piggybac

Expression

Mammalian

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pT3-EF1aH c-Met

Plasmid

#86498

Purpose

Mammalian expression of human c-Met

Depositor

Xin Chen

Article

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

Met (MET Human)

Expression

Mammalian

Promoter

EF1a

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-iCreV

Plasmid

#140135

Purpose

Can be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Has Service

AAV PHP.eB and AAV1

Insert

iCreV

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pT3-EF1a-NICD1

Plasmid

#46047

Depositor

Xin Chen

Article

Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

Insert

Notch1 (C-term) (Notch1 Mouse)

Tags

Myc tag

Expression

Mammalian

Mutation

Cytoplasmic domain initiating at amino acid 1753

Promoter

EF1a

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-mCherry

Plasmid

#114470

Purpose

mCherry Control

Depositor

Karl Deisseroth

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

mCherry

Use

AAV

Promoter

Ef1a

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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mc2 neo EF1a

Plasmid

#206220

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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