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Plasmid#42316DepositorInsertVAMP7 (1-220) (VAMP7 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX VAMP7 (1-188)
Plasmid#42315DepositorInsertVAMP7 Cytoplasmic domain (1-181) (VAMP7 Human)
UseTagsGSTExpressionBacterialMutationΔ181-220PromoterPtacAvailable sinceMarch 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP VAMP7 (1-120)
Plasmid#42317DepositorInsertVAMP7 Longin domain (1-120) (VAMP7 Human)
UseTagsEGFPExpressionMammalianMutationΔ120-220PromoterCMVAvailable sinceMarch 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1 hSlp2-a del.C2AB
Plasmid#40059DepositorInserthSlp2-a del.C2AB (SYTL2 Human)
UseTagsGSTExpressionBacterialMutationdeletion of C2A and C2B domains (601-910)PromoterTacAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1 Slp2-a C2AB
Plasmid#40058DepositorInsertSlp2-a C2AB (Sytl2 Mouse)
UseTagsGSTExpressionBacterialMutationC2AB domains onlyPromoterTacAvailable sinceNov. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pVS1
Plasmid#33143DepositorInsertLacO (256 copies)
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GVcl 1-1066 /*TBM
Plasmid#162781Purposeamplification of Gallus gallus Vcl mutated in Tankyrase Binding Domain IIDepositorArticleInsertGallus gallus vinculin (VCL Chicken)
UseTagsExpressionBacterialMutationchanged Gly 454 to ValPromoterAvailable sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
RV-mCD4-HH5-1
Plasmid#124961PurposeExpress the Helicobacter hepaticus specific TCRa/b HH5-1 in T cell hybridoma cellsDepositorInsertHH5-1
UseretroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCD/NL-BH∆1
Plasmid#41791PurposePackaging construct contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding RevDepositorInsertHIV-1 Gag/Pol and RRE sequences
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-AP-1 σ1C
Plasmid#198336PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 σ1C fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 σ1C
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationS148C substitutionPromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBAC-5xTAPE-1-BlastR
Plasmid#175808PurposeEncodes 5xTAPE-1 construct that can be integrated using piggyBAC systemDepositorInsert5xTAPE-1 construct
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-AP-1 σ1B
Plasmid#198335PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 σ1B fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 σ1B
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationExpresses a LELQMNRRY nonapeptide after the last …PromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-AP-1 σ1A
Plasmid#198334PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 σ1A fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 σ1A
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationExpresses a LELQMNRRY nonapeptide after the last …PromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-1
Plasmid#228983PurposeFor bacterial expression of anti-GST nanobody GST-1, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-1
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOAD eIF4G1 (1-341)
Plasmid#196833PurposeExpesses S.cerevisiae eIF4G1 amino acids 1-341 as Gal4 activation domain fusion.DepositorInserteIF4G1 (TIF4631 Budding Yeast)
UseTagsGal4ExpressionYeastMutationamino acids 1-341PromoterADHAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG94-1
Plasmid#228961PurposeFor bacterial expression of anti-GFP nanobody LaG94-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaG94-1
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-1
Plasmid#228974PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaTdT-1
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaRIgG-1
Plasmid#228995PurposeFor bacterial expression of anti-rabbit IgG nanobody LaRIgG-1, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaRIgG-1
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRK-ATF4 1-275
Plasmid#26118DepositorInsertATF4 (ATF4 Human)
UseTagsFLAGExpressionMammalianMutationaa's 276-351 have been deletedPromoterAvailable sinceMarch 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Blast-sgCRTC2-1
Plasmid#138700PurposeExpresses a human CRTC2-targeting sgRNA and Cas9DepositorInsertsgCRTC2 human (CRTC2 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only