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AID-C12*-dCas9-BE4max

Plasmid

#216731

Purpose

Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and dCas9 (Cas9 with D10A and H840A mutations) in mammalian cells.

Depositor

Rahul Kohli

Publication

Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

Insert

AID-C12*-dCas9-BE4max (AICDA Human)

Expression

Mammalian

Promoter

CMV

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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ER2-hfYFP

Plasmid

#186518

Purpose

Localization and retention of hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Tags

Calreticulin and KDEL

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57.1 DOX off blast NFS1

Plasmid

#160801

Purpose

Express Dox repressible NFS1

Depositor

Richard Possemato

Publication

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

NFS1 (NFS1 Human)

Use

Lentiviral

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pL-rpb1-C67Y

Plasmid

#10982

Depositor

Jeff Strathern

Publication

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-C67Y (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 200 G to A in the RPB1 ORF resulting i…

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-C70Y

Plasmid

#10983

Depositor

Jeff Strathern

Publication

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-C70Y (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 209 G to A in the RPB1 ORF resulting i…

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-H80Y

Plasmid

#10984

Depositor

Jeff Strathern

Publication

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-H80Y (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 238 C to T in the RPB1 ORF resulting i…

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-mCherry-Tandem-PSAM4-GlyR

Plasmid

#208361

Purpose

Expression of the inhibitory chemogenetic tool PSAM4-GlyR and mCherry reporter. Application of ultrapotent agonists induces neuronal silencing.

Depositor

David Bennett

Publication

Perez-Sanchez et al Sci Transl Med. 2023 Oct 4;15(716):eadh3

Insert

mCherry-TANDEM-PSAM4-GlyR

Use

AAV

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast POLD1

Plasmid

#160805

Purpose

Express POLD1

Depositor

Richard Possemato

Publication

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLD1 (POLD1 Human)

Use

Retroviral

Mutation

Codon optimized C-terminal 230 amino acids

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast POLE1 N-ISCmut

Plasmid

#160803

Purpose

Express N-terminal ISC mutant POLE1

Depositor

Richard Possemato

Publication

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLE1 N-ISCmut (POLE Human)

Use

Retroviral

Tags

3xFLAG

Mutation

shPOLE_1 and shPOLE2 resistant, C651S, C654S, C66…

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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HisGB1-CITED2(216-269)+CBPTAZ1(340-439)

Plasmid

#173761

Purpose

Bacterial coexpression of human CITED2 CTAD with CBP TAZ1

Depositor

Peter Wright

Publication

Berlow et al Nature. 2017 Mar 16;543(7645):447-451. d

Inserts

CITED2 (216-269) (CITED2 Human)
CBP TAZ1 (340-439) (Crebbp Mouse)

Expression

Bacterial

Promoter

T7

Available since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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UCP3 promoter EP1

Plasmid

#71743

Purpose

Contains 7kb of the murine UCP3 gene encompassing the core promoter, exon 1, intron 1, and part of exon 2; used to quantify promoter activity

Depositor

Heinrich Taegtmeyer

Publication

Harmancey et al J Biol Chem. 2015 Nov 10. pii: jbc.M115.

Insert

Uncoupling Protein 3 promoter (Ucp3 Mouse)

Tags

Luciferase

Expression

Bacterial

Mutation

Contains 7kb of the murine UCP3 gene encompassing…

Promoter

UCP3

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pGRNA2I_LacZB1

Plasmid

#247076

Purpose

L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets Ecoli LacZ B1 site

Depositor

Seokhee Kim

Publication

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

type I-E CRISPR cas crRNA

Use

CRISPR

Expression

Bacterial

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pVAM05

Plasmid

#252056

Purpose

Gentamicin resistant derivative of pKEK2201 for allelic exchange

Depositor

James Bina

Publication

Van Allen et al J Microbiol Methods. 2025 Jan;228:107070

Type

Empty backbone

Use

Sucrose counter-selection allelic exchange vector

Available since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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pET21a.MJ0882

Plasmid

#11417

Depositor

Sung-Hou Kim

Publication

Huang et al J Struct Funct Genomics. 2002 . 2(3):12

Insert

methyltransferase (MJ_0882 M. jannaschii)

Expression

Bacterial

Promoter

T7

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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pMut20_tadDE32B

Plasmid

#247074

Purpose

L-arabinose inducible TadDE-XTEN32-CasB(Cas11)-GSSG-UGI

Depositor

Seokhee Kim

Publication

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

TadDE-XTEN16-CasB-UGI

Use

CRISPR

Expression

Bacterial

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGH311_wtGFP S65T

Plasmid

#85408

Purpose

lentiviral expression vector for wtGFP S65T

Depositor

Michael Bassik

Publication

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

wtGFP S65T

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a.MJ0697

Plasmid

#11411

Depositor

Sung-Hou Kim

Publication

Wang et al EMBO J. 2000 Feb 1. 19(3):317-23.

Insert

fibrillarin homolog (MJ_0697 M. jannaschii)

Expression

Bacterial

Mutation

M222T

Promoter

T7

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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pMut3_pm32B

Plasmid

#247071

Purpose

L-arabinose inducible pmCDA-XTEN32-CasB(Cas11)-GSSG-UGI

Depositor

Seokhee Kim

Publication

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

PmCDA-XTEN32-Cas11(CasB)-UGI

Use

CRISPR

Expression

Bacterial

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRM200U

Plasmid

#44529

Depositor

Michael Grunstein

Publication

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

HHF2-HHT2

Expression

Yeast

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pYPQ-Cas12j2

Plasmid

#173924

Purpose

Gateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS

Depositor

Yiping Qi

Publication

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Insert

NLS-Cas12j2-NLS

Use

CRISPR

Tags

NLS

Expression

Plant

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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