Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 18,888 results for: REV

Showing: 10501 - 10550 of 18888 results

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pY71-tet.I.5

Plasmid

#133527

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 8 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pY71-tet.I.3

Plasmid

#133525

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 6 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pY71-tet.I.2

Plasmid

#133524

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 5 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pY71-tet.I.4

Plasmid

#133526

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 7 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pY71-tet.I.1

Plasmid

#133523

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 4 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFP-RIP2_S176E

Plasmid

#131203

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

Insert

RIPK2 S176E (RIPK2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S176E

Promoter

pCMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFP-RIP2_Y474F

Plasmid

#131204

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

Insert

RIPK2 Y474F (RIPK2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Y474F

Promoter

pCMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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miR-722 pCDNA

Plasmid

#97162

Purpose

luciferase assay

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

miR-722 (mir722 Zebrafish)

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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miR-722 plix4.03

Plasmid

#97140

Purpose

Lentiviral tet-inducible expression of miR-722and Dendra 2

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

miR-722 (mir722 Zebrafish)

Use

Lentiviral

Tags

dendra2

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_fly-4xUAS

Plasmid

#125149

Purpose

Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cells

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

4 x upstream activating sequence (UAS)

Use

Stap-seq screening vector

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-ATG1-ATG7 3'UTR(406)

Plasmid

#122070

Purpose

Expressing ATG1 with ATG7 3'UTR

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

serine/threonine protein kinase ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Mutation

The native 3'UTR was replaced by ATG7 3'…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pGBDU-Atg17(F317D, I325A)

Plasmid

#122275

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to Aspartic acid; Isoleucine 32…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(F317H)

Plasmid

#122269

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to Histidine

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pGBDU-Atg17(I325A)

Plasmid

#122274

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Isoleucine 325 to alanine

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(F317D, I325A)

Plasmid

#122273

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to Aspartic acid; Isoleucine 32…

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(I325A)

Plasmid

#122272

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Isoleucine 325 to alanine

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(F317Q)

Plasmid

#122271

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to glutamine

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(F317D)

Plasmid

#121391

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to Aspartic acid

Promoter

ADH1 promoter

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(F317G)

Plasmid

#122268

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to glycine

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(F317K)

Plasmid

#122270

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to lysine

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(∆335-417)

Plasmid

#121525

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

deleted amino acids 335-417*

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pESC/mCitrine/erg3-mCherry

Plasmid

#117993

Purpose

Co-expression of mCitrine under the control of the Gal1 promoter and erg3 fused to mCherry (an ER-targeting marker protein) under the control of the Gal10 promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

erg3-mCherry

Use

Plant/bacteria binary vector

Expression

Plant

Promoter

Gal10

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBDU-Atg17(L105D)

Plasmid

#121395

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Leucine 105 to Aspartic acid

Promoter

ADH1 promoter

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBDU-Atg17(I325D)

Plasmid

#121397

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Isoleucine 325 to Aspartic acid

Promoter

ADH1 promoter

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(L331D)

Plasmid

#121393

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Leucine 331 to Aspartic acid

Promoter

ADH1 promoter

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(L105D)

Plasmid

#121388

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Leucine 105 to Aspartic acid

Promoter

ADH1 promoter

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(I325D)

Plasmid

#121392

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Isoleucine 325 to Aspartic acid

Promoter

ADH1 promoter

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(I310D)

Plasmid

#121389

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Isoleucine 310 to Aspartic acid

Promoter

ADH1 promoter

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBDU-Atg17(F317D)

Plasmid

#121396

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to Aspartic acid

Promoter

ADH1 promoter

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(I98E)

Plasmid

#121387

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Isoleucine 98 to Glutamic acid

Promoter

ADH1 promoter

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(∆92-106)

Plasmid

#121517

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

deleted amino acids 92-106

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(∆313-334)

Plasmid

#121524

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

deleted amino acids 313-334

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(∆296-312)

Plasmid

#121523

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

deleted amino acids 296-312

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(∆279-295)

Plasmid

#121522

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

deleted amino acids 279-295

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(∆129-177)

Plasmid

#121518

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

deleted amino acids 129-177

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(∆266-280)

Plasmid

#121520

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

deleted amino acids 266-280

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(∆15-106)

Plasmid

#121515

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

deleted amino acids 15-106

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(∆186-236)

Plasmid

#121519

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

deleted amino acids 186-236

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(∆266-312)

Plasmid

#121521

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

deleted amino acids 266-312

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC 3GLA

Plasmid

#71762

Purpose

gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant

Depositor

Matthias Soller

Article

Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

Type

Empty backbone

Expression

Insect

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRPL28 SpHIS5 (pNTI663)

Plasmid

#115433

Purpose

Expresses wild-type RPL28 from HIS3 yeast plasmid

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
HIS5

Expression

Yeast

Mutation

MfeI site in intron

Promoter

Ashbya gospii TEF1 and endogenous

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRPL28(Q38P) SpHIS5 (pNTI664)

Plasmid

#115434

Purpose

Expresses Q38P RPL28 from HIS3 yeast plasmid

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
HIS5

Expression

Yeast

Mutation

Q38P; MfeI site in intron

Promoter

Ashbya gospii TEF1 and endogenous

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRPL28(H39L) SpHIS5 (pNTI665)

Plasmid

#115435

Purpose

Expresses H39L RPL28 from HIS3 yeast plasmid

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
HIS5

Expression

Yeast

Mutation

H39L; MfeI site in intron

Promoter

ashbya gospii TEF1 and endogenous

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
TIAM2_PDZ_1

Plasmid

#103952

Purpose

Protein expression and purification of TIAM2 PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

TIAM2 (TIAM2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
SNTA1_PDZ_1

Plasmid

#103928

Purpose

Protein expression and purification of STNA1 PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

SNTA1 (SNTA1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MPP1_PDZ_1

Plasmid

#103931

Purpose

Protein expression and purification of MPP1 PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

MPP1 (MPP1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
INADL_PDZ_5

Plasmid

#103905

Purpose

Protein expression and purification of INADL PDZ5 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

INADL (PATJ Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
INADL_PDZ_6

Plasmid

#103906

Purpose

Protein expression and purification of INADL PDZ6 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

INADL (PATJ Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
INADL_PDZ_7

Plasmid

#103907

Purpose

Protein expression and purification of INADL PDZ7 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

INADL (PATJ Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MPP6_PDZ_1

Plasmid

#103924

Purpose

Protein expression and purification of P55 PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

MPP6 (PALS2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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