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Showing: 10501 - 10550 of 11549 results

  1. TL1 alphaDdCBE 3_NC (left, DddA11-N)

    Plasmid
    #222004
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-3_NC left)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A | DddA: S1330I, A1341V,…
    Promoter
    CMV
    Available Since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222004-plasmid-map-sequence-id-443120

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  2. pTE5418_pMBP-GlcR

    Plasmid
    #221192
    Purpose
    Expression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli.
    Depositor
    Tobias Erb
    Article
    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0
    Insert
    glcR (pden4400 from Paracoccus denitrificans)
    Tags
    10x His tag with E. coli maltose binding protein …
    Expression
    Bacterial
    Promoter
    T7 promoter and T7 promoter-lacO
    Available Since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221192-plasmid-map-sequence-id-446698

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  3. Tol2-U6.3-sgRNA-non-targeting-control -GFP

    Plasmid
    #221844
    Purpose
    Tol2 transposon expresses control non-targeting sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter
    Depositor
    Stephen Terry
    Article
    O'Sullivan et al Development. 2024 Aug 1;151(15):dev20284
    Inserts
    EGFP
    non-targeting control sgRNA- GCACTGCTACGATCTACACC
    Use
    CRISPR; Tol2 transposon optimised for chick expre…
    Expression
    Mammalian
    Promoter
    GACG and U6.3 chick
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    221844-plasmid-map-sequence-id-437475

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  9. pRAR051

    Plasmid
    #185029
    Purpose
    400 nt l31 promoter region, l31 5'UTR with nt. 47-51 and 80-84 mutated, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB
    Depositor
    Julius Lucks
    Article
    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127
    Insert
    L31-sfGFP
    Expression
    Bacterial
    Mutation
    Nucleotides 47-51 & 80-84 mutated rpmE 5'…
    Available Since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    185029-plasmid-map-sequence-id-363327

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  10. pRAR052

    Plasmid
    #185030
    Purpose
    400 nt l31 promoter region, l31 5'UTR with nt. 56-61 and 68-73 mutated, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB
    Depositor
    Julius Lucks
    Article
    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127
    Insert
    L31-sfGFP
    Expression
    Bacterial
    Mutation
    Nucleotides 56-61 and 68-73 mutated in rpmE 5…
    Available Since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    185030-plasmid-map-sequence-id-366943

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  16. pRAR039

    Plasmid
    #185025
    Purpose
    400 nt l31 promoter region, l31 5'UTR with nt. 1-31 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB
    Depositor
    Julius Lucks
    Article
    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127
    Insert
    L31-sfGFP
    Expression
    Bacterial
    Mutation
    Nucleotides 1-31 deleted from rpmE 5'UTR, On…
    Available Since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    185025-plasmid-map-sequence-id-366941

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  17. pRAR040

    Plasmid
    #185026
    Purpose
    400 nt l31 promoter region, l31 5'UTR with nt. 35-46 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB
    Depositor
    Julius Lucks
    Article
    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127
    Insert
    L31-sfGFP
    Expression
    Bacterial
    Mutation
    Nucleotides 35-46 deleted from rpmE 5'UTR, O…
    Available Since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    185026-plasmid-map-sequence-id-363325

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  18. pRAR041

    Plasmid
    #185027
    Purpose
    400 nt l31 promoter region, l31 5'UTR with nt. 47-54 & 76-86 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB
    Depositor
    Julius Lucks
    Article
    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127
    Insert
    L31-sfGFP
    Expression
    Bacterial
    Mutation
    Nucleotides 47-54 & 76-86 deleted from rpmE …
    Available Since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    185027-plasmid-map-sequence-id-374681

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  24. pBCC086

    Plasmid
    #202213
    Purpose
    Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlum
    Depositor
    Paul Schulze-Lefert
    Article
    Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.
    Insert
    pBCC25, pBCC33, pBCC55, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
    Expression
    Bacterial
    Available Since
    April 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    202213-plasmid-map-sequence-id-403467

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  25. pBCC091

    Plasmid
    #202218
    Purpose
    Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum
    Depositor
    Paul Schulze-Lefert
    Article
    Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.
    Insert
    pBCC25, pBCC30, pBCC55, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
    Expression
    Bacterial
    Available Since
    April 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    202218-plasmid-map-sequence-id-403465

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  26. pBCC100

    Plasmid
    #202226
    Purpose
    Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Kanamycin selectable marker and far-red-FP fluorescent marker mPlum
    Depositor
    Paul Schulze-Lefert
    Article
    Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.
    Insert
    pBCC25, pBCC32, pBCC55, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
    Expression
    Bacterial
    Available Since
    April 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    202226-plasmid-map-sequence-id-403464

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  27. pCF702

    Plasmid
    #209650
    Purpose
    Suicide plasmid carrying a neomycin resistance marker flanked by homologous regions of the Mth60-fimbria encoding operon of Methanothermobacter thermautotrophicus
    Depositor
    Bastian Molitor
    Article
    Fink et al Appl Environ Microbiol. 2023 Jul 26;89(7
    Inserts
    Downstream homologous region
    thermostable neomycin phosphotransferase gene
    Upstream homologous region
    Available Since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    209650-plasmid-map-sequence-id-416212

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  29. pYG215

    Plasmid
    #200837
    Purpose
    Transcribes a glmZ' (1-146)-gfp fusion. gfp can be replaced via AgeI/XbaI-sites with gene of interest to release its RNA with a 5' monophosphate upon cleavage.
    Depositor
    Boris Görke
    Article
    Schilder et al Nucleic Acids Res. 2023 Mar 29:gkad226.
    Insert
    glmZ (glmZ Escherichia coli str. K-12 substr. MG1655)
    Use
    Synthetic Biology
    Tags
    GFP
    Expression
    Bacterial
    Promoter
    P-LlacO-1
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200837-plasmid-map-sequence-id-405511

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  45. pRB116.2

    Plasmid
    #181946
    Purpose
    aTc-inducible expression of TorR with C-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129
    Depositor
    Jeffrey Tabor
    Article
    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11
    Insert
    TorR-mNG; mCherry; tetR (AX04_RS23485, torR Synthetic, torR - E. coli)
    Tags
    mNeonGreen
    Expression
    Bacterial
    Promoter
    TorR-mNG-PLtetO-1; mCherry-PtorCAD129; tetR-J23106
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181946-plasmid-map-sequence-id-356600

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  46. pRB120.2

    Plasmid
    #181947
    Purpose
    aTc-inducible expression of TorR with N-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129
    Depositor
    Jeffrey Tabor
    Article
    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11
    Insert
    mNG-TorR; mCherry; tetR (torR, AX04_RS23485 Synthetic, torR - E. coli)
    Tags
    mNeonGreen
    Expression
    Bacterial
    Promoter
    mNG-TorR-PLtetO-1; mCherry-PtorCAD129; tetR-J23106
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181947-plasmid-map-sequence-id-356558

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  47. pRB133.2

    Plasmid
    #181950
    Purpose
    aTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirK
    Depositor
    Jeffrey Tabor
    Article
    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11
    Insert
    PhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 Synthetic, PhoP-Salmonella enterica subsp. enterica serovar Typhimurium)
    Tags
    mNeonGreen
    Expression
    Bacterial
    Promoter
    PhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181950-plasmid-map-sequence-id-356579

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  48. pPbuCas13b-gRNA-1

    Plasmid
    #184567
    Purpose
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    184567-plasmid-map-sequence-id-374002

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  49. pSBK.136

    Plasmid
    #187220
    Purpose
    Expresses Eco1 ncRNA v35 (long a1/a2) and Eco1 ncRNA v35 (long a1/a2, barcode 6) from promoters pSalTTC and pTet*, respectively
    Depositor
    Seth Shipman
    Article
    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:
    Insert
    Retron Eco1 ncRNA v35 (long a1/a2); Retron Eco1 ncRNA v35 (long a1/a2, barcode 6)
    Expression
    Bacterial
    Promoter
    A: pSalTTC; B: pTet*
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    187220-plasmid-map-sequence-id-368082

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Showing: 10501 - 10550 of 11549 results