We narrowed to 152,943 results for: des

1. pRS306-PTDH3-SUMO7-6SIM

   Plasmid

   #190250

   Purpose

   Expression SUMO7-SIM6 in yeast cells

   Depositor

   Liam Holt

   Article

   Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

   Insert

   7SUMO-6SIM
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS304-PTDH3-HEXAMER

   Plasmid

   #190251

   Purpose

   Expression hexamer component in yeast

   Depositor

   Liam Holt

   Article

   Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

   Insert

   hexamer
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Paxillin DN

   Plasmid

   #186151

   Purpose

   For expressing recombinant paxillin variants in bacteria.

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   PXN (PXN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Paxilln residues 1-321 added to N-terminus of WT …

   Promoter

   Lac

   Available since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMC_cherry donor_Rosa target (pE002)

   Plasmid

   #149348

   Purpose

   Replace donor vector for cherry insertion using the mouse Rosa26 guide RNA

   Depositor

   Ralf Kuehn

   Article

   Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

   Insert

   cherry
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMC_cherry donor_Polb target (pE038)

   Plasmid

   #149349

   Purpose

   Replace donor vector for cherry insertion using the human Polb guide RNA

   Depositor

   Ralf Kuehn

   Article

   Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

   Insert

   cherry
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCTCON2-GXB

   Plasmid

   #160926

   Purpose

   Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Inserts

   BFP
   sfGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCTCON2-Aga1p-FAPB2.3.6L1TAG

   Plasmid

   #158143

   Purpose

   Yeast display reporter for non-display yeast strains (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   FAPB2.3.6L1TAG
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAF302

   Plasmid

   #137752

   Purpose

   Intracellular control plasmid for HiBiTopt experiments

   Depositor

   Wiep Klaas Smits

   Article

   Oliveira Paiva et al J Bacteriol. 2020 Aug 31. pii: JB.00771-

   Insert

   hupA-linker-hibitopt
   

   Use

   Tags

   HiBiTopt

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAP233

   Plasmid

   #137753

   Purpose

   Extracellular control for HiBiTopt experiments

   Depositor

   Wiep Klaas Smits

   Article

   Oliveira Paiva et al J Bacteriol. 2020 Aug 31. pii: JB.00771-

   Insert

   srtB-linker-hibitopt
   

   Use

   Luciferase

   Tags

   HiBiTopt

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDRF1-GW ymVenus-T2A-mTurquoise2

    Plasmid

    #118443

    Purpose

    Produces equimolar expression of yeast codon-optimized mVenus and mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymVenus-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDRF1-GW yotagRFPT-T2A-mTurquoise2

    Plasmid

    #118449

    Purpose

    Produces equimolar expression of yeast codon-optimized tagRFPT and mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    yotagRFPT-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEPOR2KN0012

    Plasmid

    #117584

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-DE(pEPOR1CB0008) and sgRNA_NbPDS(pEPOR1CB0114)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-DE(pEPOR1CB0008) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEPOR0SP0001

    Plasmid

    #117523

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-DE (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-DE (no stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D1135E

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVH011

    Plasmid

    #80395

    Purpose

    Contains bicistronic pTet-Scaffold(3xFLAG)- ShineDelgarno-mCherry and TetR genes. Used for Western blots.

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Insert

    Bicistronic Scaffold-3xFLAG-SD-mCherry
    

    Use

    Synthetic Biology

    Tags

    3xFLAG tag on scaffold protein for Western

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRAJ12f

    Plasmid

    #39248

    Depositor

    Alfonso Jaramillo

    Article

    Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

    Insert

    RAJ12 system (no terminator for the transRNA)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLlacO-1+pLtetO-1

    Available since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRAJ11-12

    Plasmid

    #39258

    Depositor

    Alfonso Jaramillo

    Article

    Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

    Insert

    transRAJ11+cisRAJ12
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLlacO-1+pLtetO-1

    Available since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1-blast-cfRab8b_1

    Plasmid

    #26708

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

    Insert

    dog Rab8b RNAi
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Dog Rab8b RNAi

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

18. PLVX_SPTA1_IGF2BP1_IRES_PURO

    Plasmid

    #100016

    Purpose

    To over-express IGF2BP1 in Adult Erythroblasts using tissue specific promoter with IRES for puromycin selection.

    Depositor

    Jeffery Miller

    Article

    de Vasconcellos et al Proc Natl Acad Sci U S A. 2017 Jul 11;11

    Insert

    Insulin like growth factor 2 mRNA binding protein 1 (IGF2BP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SPTA1 erythroid specific promoter

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGfa2(ABDx3)-nLac

    Plasmid

    #53133

    Purpose

    expression of nuclear LacZ driven by Gfa2 promoter with 3 additional copies of the gfa28 ABD segment; has about 100 times higher activity than pGfa2-nLac in transfected cells

    Depositor

    Michael Brenner

    Article

    de Leeuw et al J Neurosci Res. 2006 Apr;83(5):744-53.

    Insert

    Gfa2 (GFAP Human)
    

    Use

    Mouse Targeting

    Tags

    LacZ, NLS, and mP1

    Expression

    Mammalian

    Mutation

    -2163 to +47, with 3 copies of 3 copies of the gf…

    Promoter

    human Gfa2

    Available since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-pGK-nAChRb2(E61C)-IRES-GFP

    Plasmid

    #164777

    Purpose

    cysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligand

    Depositor

    Alexandre Mourot

    Article

    Durand-de Cuttoli et al Elife. 2018 Sep 4;7. pii: 37487. doi: 10

    Insert

    nAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    E61C

    Promoter

    pGK

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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