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We narrowed to 14,359 results for: cas9

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Showing: 4221 - 4240 of 14359 results

  1. pLenti gRNA KO OCLNx2 spCas9-T2A-iRFP670-P2A-puro

    Plasmid
    #208399
    Purpose
    codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, iRFP670 fluorescent protein and puromycin resistance
    Depositor
    Raphael Gaudin
    Publication
    Pradel et al Autophagy. 2024 Aug;20(8):1825-1836. doi
    Insert
    hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
    Use
    CRISPR and Lentiviral
    Tags
    iRFP670
    Expression
    Mammalian
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    208399-plasmid-map-sequence-id-417652

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  2. pLenti gRNA KO OCLNx2 spCas9-T2A-Crimson-P2A-puro

    Plasmid
    #208398
    Purpose
    codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, E2-Crimson fluorescent protein and puromycin resistance
    Depositor
    Raphael Gaudin
    Publication
    Pradel et al Autophagy. 2024 Aug;20(8):1825-1836. doi
    Insert
    hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
    Use
    CRISPR and Lentiviral
    Tags
    E2-Crimson
    Expression
    Mammalian
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    208398-plasmid-map-sequence-id-417651

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  3. pLenti gRNA KO OCLNx2 spCas9-T2A-mNeonGreen-P2A-puro

    Plasmid
    #208400
    Purpose
    codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, mNeonGreen fluorescent protein and puromycin resistance
    Depositor
    Raphael Gaudin
    Publication
    Pradel et al Autophagy. 2024 Aug;20(8):1825-1836. doi
    Insert
    hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
    Use
    CRISPR and Lentiviral
    Tags
    mNeonGreen
    Expression
    Mammalian
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    208400-plasmid-map-sequence-id-417650

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  9. pCas9CR4-NG

    Plasmid
    #120225
    Purpose
    pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Tags
    ssrA
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120225-plasmid-map-sequence-id-233264

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  10. Fungal Toolkit for Modular Cloning (FTK)

    Plasmid kit
    #1000000191
    Purpose
    Synthetic biology entry vectors with ready-to-use fungal genetic parts for rapid construction of genetic circuits as well as CRISPR components for efficient genome engineering in filamentous fungi.
    Depositor
    Arnold Driessen
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector type
    Fungal Expression
    Editing type
    CRISPR
    Cloning type
    Golden Gate
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only

  12. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196080
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Publication
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Blasticidin S deaminase
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196080-plasmid-map-sequence-id-388988

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  13. pTRE3G-dCas9-VP64-MS2-VP64-ZsG

    Plasmid
    #192669
    Purpose
    Dox-inducible expression of dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    dCas9-VP64, MS2-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    TRE3G
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192669-plasmid-map-sequence-id-379887

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  14. Lenti-EF1alpha-VP64-dCas9-VP64-ZsG

    Plasmid
    #192667
    Purpose
    3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    VP64-dCas9-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1a
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192667-plasmid-map-sequence-id-379870

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  15. nCas9-UGI-NLS-P2A-EGFP (pJUL1001)

    Plasmid
    #123611
    Purpose
    CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1).
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nCas9-UGI-NLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A in SpCas9
    Promoter
    CAG
    Available since
    April 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    123611-plasmid-map-sequence-id-239339

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  16. dCas9-10xSunTag-P2A-tagBFP

    Plasmid
    #174141
    Purpose
    Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.
    Depositor
    Albert Jeltsch
    Publication
    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9, 10xSunTag, tagBFP
    Tags
    HA and tagBFP
    Expression
    Mammalian
    Promoter
    SV40
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    174141-plasmid-map-sequence-id-345615

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  19. pdCas9-sgRNA-com-BoxB-vector

    Plasmid
    #132556
    Purpose
    Expresses dCas9-DMNT3a-DMNT3l fusion protein, lambda N22-KRAB fusion protein, and sgRNA scaffold with com and BoxB replacing the Tetraloop and the loop 2 respectively.
    Depositor
    Baisong Lu
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-DMNT3a-DMNT3l (DNMT3A Synthetic)
    Lambda N22-KRAB
    sgRNA acaffold with com and BoxB replacing the Tetraloop and the loop 2.
    Expression
    Mammalian
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    132556-plasmid-map-sequence-id-261537

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  20. GCN5 core-dCas9-CBP core

    Plasmid
    #179555
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179555-plasmid-map-sequence-id-354784

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Showing: 4221 - 4240 of 14359 results