We narrowed to 15,156 results for: pET

1. pnEA-NpM-DmThor_50-83-R56ER63E-GB1_AA

   Plasmid

   #148272

   Purpose

   Bacterial Expression of DmThor_50-83-R56ER63E

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

   Insert

   DmThor_50-83-R56ER63E (Thor Fly)
   

   Expression

   Bacterial

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7-EGFP-C1-HsNot7-M141R_R

   Plasmid

   #147379

   Purpose

   Mammalian Expression of HsNot7-M141R

   Depositor

   Elisa Izaurralde

   Article

   Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

   Insert

   HsNot7-M141R (CNOT7 Human)
   

   Expression

   Mammalian

   Available Since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pnEA-NvG-HsNot7_9-260_R

   Plasmid

   #147369

   Purpose

   Bacterial Expression of HsNot7_9-260

   Depositor

   Elisa Izaurralde

   Article

   Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

   Insert

   HsNot7_9-260 (CNOT7 Human)
   

   Expression

   Bacterial

   Available Since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. RF15

   Bacterial Strain

   #102799

   Purpose

   BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB glyA serB genes deleted.

   Depositor

   Robert Gennis, Toshio Iwasaki

   Article

   Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

   Bacterial Resistance

   None

   Available Since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

5. RF16

   Bacterial Strain

   #102800

   Purpose

   BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB glyA serB cysE genes deleted.

   Depositor

   Robert Gennis, Toshio Iwasaki

   Article

   Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

   Bacterial Resistance

   None

   Available Since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

6. NA20 LN16

   Plasmid

   #101870

   Purpose

   Expresses HIV Env gene when transfected into 293-T cells for production of pseudovirus

   Depositor

   Paul Clapham

   Article

   Peters et al J Virol. 2004 Jul;78(13):6915-26.

   Insert

   Env (env )
   

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-CAG-H2BmGreenLantern

   Plasmid

   #177332

   Purpose

   To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.

   Depositor

   Pantelis Tsoulfas, Murray Blackmore, Gregory Petsko

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   H2B (Hist1h2bq Rat)
   

   Use

   AAV

   Tags

   mGreenLantern

   Expression

   Mammalian

   Mutation

   This fusion protein was optimized to the Human co…

   Promoter

   CAG

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5 FRT TO GFP11-LRRK2

   Plasmid

   #231174

   Purpose

   Expresses GFP-11-LRRK2 (wildtype, full length) in mammalian cells

   Depositor

   Samara Reck-Peterson

   Article

   Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

   Insert

   GFP11-LRRK2 (LRRK2 Human)
   

   Tags

   GFP11

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-DIO-mGreenLantern

   Plasmid

   #164468

   Purpose

   Cre-dependent EF1a-driven expression of mGreenLantern in animals using AAV

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   EF1a

   Available Since

   Jan. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. PLX_307 FDX1

    Plasmid

    #184495

    Purpose

    Lentivirus expression for FDX1

    Depositor

    Todd Golub, Peter Tsvetkov

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    FDX1 (FDX1 Human)
    

    Use

    Lentiviral

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLM-vexGFP-Oct4

    Plasmid

    #22240

    Purpose

    2nd generation lentiviral vector

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    vexGFP_2A_OCT4 (POU5F1 Human, synthetic fluorescent protein)
    

    Use

    Lentiviral

    Available Since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. gRNA-10Xcapture-PuroR

    Plasmid

    #211496

    Purpose

    Plasmid for the expression of SAM-compatible gRNA for CRISPRa with capturer sequence for 10X Genomics sequencing

    Depositor

    Antonella Fidanza, Lesley Forrester

    Article

    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

    Inserts

    U6-gRNA
    Ef1a-PuroR
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1a and U6

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3 CDK9 (D167N) HA (P#811)

    Plasmid

    #14641

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

    Insert

    cdk9 D167N (CDK9 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D167N. Kinase negative mutant. Please see deposit…

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT2-TetR-neoR

    Plasmid

    #153310

    Purpose

    Tet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GW

    Depositor

    Spyros Petrakis

    Article

    Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

    Insert

    TetR
    

    Use

    Transposon

    Promoter

    SV40

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5 FLAG DEPTOR (13×S/T->A)

    Plasmid

    #21702

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

    Insert

    DEPTOR 13xA (DEPTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Nonphosphorylatable mutant. 13xA. Also has N204S …

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLINK cyclin T1 (1-280) myc (K93D) (P#580)

    Plasmid

    #14611

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

    Insert

    cyclin T1 (1-280) K93D (CCNT1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    K93D. Deficient in binding to CDK9. Inactive in 3…

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pLKO mouse shRNA 1 DEPTOR

    Plasmid

    #21337

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

    Insert

    DEPTOR shRNA 1 (Deptor Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 7, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pT2-CMV/TetO2-EYFP-GW

    Plasmid

    #153309

    Purpose

    Tet-On YFP-tagged Gateway destination vector, to be used together with pT2-TetR-neoR transposon plasmid

    Depositor

    Spyros Petrakis

    Article

    Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Yellow Fluorescent Protein

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCytERM-mGreenLantern

    Plasmid

    #164467

    Purpose

    Visualization of the ER in cells (compatible with OSER assay)

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Tags

    Cytochrome p450 first 29 animo acids (Oryctolagus…

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-EGFP-C1-HsNot1iso1-V1251R_R

    Plasmid

    #147376

    Purpose

    Mammalian Expression of HsNot1iso1-V1251R

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot1iso1-V1251R (CNOT1 Human)
    

    Expression

    Mammalian

    Mutation

    A deletion of 5 AA 822-827 SKMKPS-> T and one …

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLKO mouse shRNA 2 DEPTOR

    Plasmid

    #21338

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

    Insert

    DEPTOR shRNA 2 (Deptor Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only

22. pLINK cyclin T1 (1-280) myc (all 200) (P#575)

    Plasmid

    #14610

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

    Insert

    cyclin T1 (1-280) (all 200) (CCNT1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    C198A, C200A, H202A, C205A. Active in 3T3 cells.

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA5 FRT to BioID-nDIC2-3xFlag

    Plasmid

    #232418

    Purpose

    Expresses human dynein subunit DIC2 in mammalian cells.

    Depositor

    Samara Reck-Peterson

    Article

    Redwine et al Elife. 2017 Jul 18;6:e28257. doi: 10.755

    Insert

    Dynein Intermediate Chain 2 (DYNC1I2 Human)
    

    Tags

    BioID and Flag-3x

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA5 FRT to BioID-cDIC2-3xFlag

    Plasmid

    #232416

    Purpose

    Expresses human dynein subunit DIC2 in mammalian cells.

    Depositor

    Samara Reck-Peterson

    Article

    Redwine et al Elife. 2017 Jul 18;6:e28257. doi: 10.755

    Insert

    Dynein Intermediate Chain 2 (DYNC1I2 Human)
    

    Tags

    BioID and Flag-3x

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAc5.1B-lambdaN-HA-DmThor_50-83-YMVLtoAADD_AB

    Plasmid

    #148376

    Purpose

    Insect Expression of DmThor_50-83-YMVLtoAADD

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    DmThor_50-83-YMVLtoAADD (Thor Fly)
    

    Expression

    Insect

    Mutation

    two silent mutations compared to the sequence giv…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAc5.1B-lambdaN-HA-DmThor-V75DL79D_AB

    Plasmid

    #148377

    Purpose

    Insect Expression of DmThor-V75DL79D

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    DmThor-V75DL79D (Thor Fly)
    

    Expression

    Insect

    Mutation

    two silent mutations compared to the sequence giv…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAc5.1B-EGFP-DmThor-S65DT70D_AB

    Plasmid

    #148375

    Purpose

    Insect Expression of DmThor-S65DT70D

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    DmThor-S65DT70D (Thor Fly)
    

    Expression

    Insect

    Mutation

    two silent mutations compared to the sequence giv…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pT7-EGFP-C1-HsNot1iso1-4x mut_R

    Plasmid

    #147371

    Purpose

    Mammalian Expression of HsNot1iso1-4xmut

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot1iso1-4xmut (CNOT1 Human)
    

    Expression

    Mammalian

    Mutation

    A deletion of 5 AA 822-827 SKMKPS-> T and one …

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. pT7-EGFP-C1-HsNot1iso1-K1208AH1212AK1218A_R

    Plasmid

    #147372

    Purpose

    Mammalian Expression of HsNot1iso1-K1208AH1212AK1218A

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot1iso1-K1208AH1212AK1218A (CNOT1 Human)
    

    Expression

    Mammalian

    Mutation

    A deletion of 5 AA 822-827 SKMKPS-> T and one …

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pT7-EGFP-C1-HsNot1iso1-K1208EH1212TK1218E_R

    Plasmid

    #147373

    Purpose

    Mammalian Expression of HsNot1iso1-K1208EH1212TK1218E

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot1iso1-K1208EH1212TK1218E (CNOT1 Human)
    

    Expression

    Mammalian

    Mutation

    A deletion of 5 AA 822-827 SKMKPS-> T and one …

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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