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Golgi-targeted optoPLD

Plasmid

#140058

Purpose

Encodes CRY2-mCherry-PLD-P2A-CIBN-Sialyltransferase(TM) for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Tags

CIBN, CRY2, P2A, Sialyltransferase(TM), and mCher…

Expression

Mammalian

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pTK_Sox10_enh_99_Citrine

Plasmid

#130588

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox10_enh_99

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pC1-CMV-DAAO-NES

Plasmid

#141107

Purpose

Expresses DAAO with nuclear export signal in mammalian cells

Depositor

Vsevolod Belousov

Article

Bogdanova et al Curr Protoc Chem Biol. 2017 Jun 19;9(2):

Insert

DAAO-NES

Tags

Nuclear export signal

Expression

Mammalian

Mutation

deleted DAAO amino acids 367-368

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pTK_Sox10_enh_89_Citrine

Plasmid

#130587

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox10_enh_89

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pNL [NlucP/CEBP-RE/Hygro] Vector

Plasmid

#236832

Purpose

Express CEBP response element in luciferase reporter assay

Depositor

Promega Corporation

Has Service

DNA

Insert

CEBP (CEBPA Human)

Use

Luciferase

Tags

NanoLuc (R)

Mutation

None

Promoter

minP

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pTiger-OptoSOS

Plasmid

#86439

Purpose

Single-construct blue light optogenetic Ras activator for use in Drosophila

Depositor

Jared Toettcher

Article

Johnson et al Dev Cell. 2017 Jan 23;40(2):185-192. doi

Insert

OptoSOS

Tags

tagRFP

Expression

Insect

Promoter

Gal4-UAS

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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JGI_018319

Plasmid

#158837

Purpose

Rare earth element alcohol dehydrogenases

Depositor

Joint Genome Institute

Article

Huang et al ISME J. 2019 Aug;13(8):2005-2017. doi: 1

Insert

Mex_1740

Use

Synthetic Biology

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-AncBE4max

Plasmid

#138270

Purpose

Expression of AncBE4max-Cas9 Base-editor from an EF1a promoter.

Depositor

Xiao Wang

Article

Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

Insert

AncBE4max

Use

CRISPR

Expression

Mammalian

Mutation

D10A

Promoter

Ef1a

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Myo19

Plasmid

#127612

Purpose

GFP-tagged Myosin-19

Depositor

Josef Kittler

Article

Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

Insert

Myo19

Tags

eGFP

Expression

Mammalian

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-mCh-kCAAX

Plasmid

#112267

Purpose

ITRACK Acceptor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

mCherry with KRas membrane targeting domain

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCMX/cyclinB1 (17)

Plasmid

#26060

Depositor

Jonathon Pines

Article

Hagting et al Curr Biol. 1999 Jul 1. 9(13):680-9.

Insert

Cyclin B1 (CCNB1 Human)

Tags

Myc

Expression

Mammalian

Available Since

Aug. 27, 2010

Availability

Academic Institutions and Nonprofits only
pEPIC1.1

Plasmid

#169897

Purpose

Empty constitutive expression vector with piggyBac, IRES, and mClover3

Depositor

Richard Schneider

Article

Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

Type

Empty backbone

Use

Avian expression

Tags

3x FLAG 2x StrepII

Expression

Mammalian

Promoter

mouse PGK

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pGagMBPHis

Plasmid

#42176

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

Gag protein sequence is from HIV-1 clone NL4-3

Tags

6xHIS and MBP

Expression

Bacterial

Available Since

July 25, 2013

Availability

Academic Institutions and Nonprofits only
VV073: 1xCox8 - GCaMP5G in fubi

Plasmid

#58509

Purpose

Expresses the calcium sensor GCaMP5G in the mitochondria

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - GCaMP5G

Use

Lentiviral

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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LRT2B-AdGO2

Plasmid

#136911

Purpose

Lentivirus expressing AdGO2 sgRNA

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

sgAdGO2

Use

Lentiviral

Mutation

WT

Promoter

hU6

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB601NL3F10H

Plasmid

#141287

Purpose

No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, BASTA R for plant)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Type

Empty backbone

Use

Gateway Cloning and Luciferase

Tags

FLAG (x3), HIS (x10), and NLUC

Expression

Plant

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAG364-greenFAST

Plasmid

#160353

Purpose

expresses greenFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

greenFAST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMslc

Plasmid

#32095

Depositor

Christopher Buck

Article

Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

Insert

SLC35A1 (SLC35A1 Human)

Expression

Mammalian

Promoter

FerH

Available Since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pAG365-redFAST

Plasmid

#160354

Purpose

Expresses redFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

redFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBSU6-shScr/CMV-eGFP

Plasmid

#89912

Purpose

Express shRNA Scrambled control

Depositor

Robert Friedlander

Article

Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

Insert

shRNA Scrambled

Tags

eGFP under separate promoter

Expression

Mammalian

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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