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pcDNA5/FRT/TO - RBM28 E5 WT minigene

Plasmid

#169269

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 5 WT minigene

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 E5 WT minigene (RBM28 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pB-puro-mU6-CNPsgRNA

Plasmid

#126610

Purpose

Expresses CNP sgRNA under mouse U6 promoter

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

CNP sgRNA (Cnp Mouse)

Use

CRISPR

Promoter

mouse U6 promoter

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pip85.A

Plasmid

#98933

Purpose

in vitro pre-mRNA splicing substrate

Depositor

Melissa Moore, Phil Sharp

Article

Moore et al Science. 1992 May 15;256(5059):992-7.

Insert

PIP85.A

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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K48R FLAG-HaloTag-RTEL1_DonorVector

Plasmid

#247171

Purpose

HR Donor for K48R C-terminal FLAG-HaloTag-RTEL1 fusion in human cells. Includes Puro selection marker.

Depositor

Thomas Cech

Article

Wu et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

K48R FLAG-HaloTag-RTEL1 (RTEL1 Human)

Promoter

Endogenous RTEL1 promoter

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-BioID2-BioID2-Kir4.1-HA

Plasmid

#176746

Purpose

Expresses Kir4.1 fused with BioID2 at the N-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

HA-BioID2-BioID2-Kir4.1 (Kcnj10 Rat, Aquifex aeolicus)

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pTH33 [pmyo-3::YFP-BeCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168169

Purpose

Expression of YFP-BeCyclOp(A-2x) in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)

Tags

YFP

Expression

Worm

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-OPNb-delta S

Plasmid

#107984

Purpose

bacterial expression of OPN splice variant b

Depositor

Georg Weber

Article

He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

Insert

OPNb 1038bp-17aa signal (SPP1 Human)

Tags

GST

Expression

Bacterial

Mutation

WT splice variant

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-OPNc-delta S

Plasmid

#107985

Purpose

bacterial expression of OPN splice variant c

Depositor

Georg Weber

Article

He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

Insert

OPNc 999bp-16aa signal (SPP1 Human)

Tags

GST

Expression

Bacterial

Mutation

WT splice variant

Available Since

May 7, 2018

Availability

Academic Institutions and Nonprofits only

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MPC11 IgG2b gene

Plasmid

#127248

Purpose

Expresses intact IgG2b gene in mammalian cells

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

IgG2b gene with exons and alt pAs (Ighg2b Mouse)

Expression

Mammalian

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Pcamr-RFP

Plasmid

#190614

Purpose

Expresses RFP with an engineered CamR-regulated promoter in E.coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al ACS Synth Biol. 2022 Jan 5. doi: 10.1021

Insert

mScarlet-i

Use

Synthetic Biology

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pZA1pLtetO-GFP

Plasmid

#78227

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pReg-FEN2

Plasmid

#190616

Purpose

Expresses a generalist biosensor responsive to bicyclic monoterpenes in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al ACS Synth Biol. 2022 Jan 5. doi: 10.1021

Insert

FEN2

Use

Synthetic Biology

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pWS3806

Plasmid

#185747

Purpose

Sub-array spacer 2/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 2/4

Use

CRISPR

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pWS3808

Plasmid

#185749

Purpose

Sub-array spacer 4/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 4/4

Use

CRISPR

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pETM30-C9ORF78_F8A

Plasmid

#193369

Purpose

Expression of C9ORF78 F8A variant in E.coli with N-terminal His6-GST-tag which is cleavable with TEV protease

Depositor

Markus Wahl

Article

Bergfort et al Nat Commun. 2022 Mar 3;13(1):1132. doi:

Insert

C9ORF78_F8A (C9orf78 Human)

Tags

His6-GST-TEV

Expression

Bacterial

Mutation

F8A, the mutation is located in the binding inter…

Promoter

T7

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCW-Cdt1ΔCy-mCherry-Puro

Plasmid

#193764

Purpose

Doxycyline-inducible (TetOn) human Cdt1ΔCy (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

Cdt1ΔCy-mCherry (CDT1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

Cy motif (a.a. 68-70) mutated to alanine, a.a. 11…

Promoter

TRE

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-Egr3-EYFP-WPRE

Plasmid

#186417

Purpose

Overexpression of Egr3 transcript variant 1 CDS under EF-1α promoter

Depositor

Mary Kay Lobo

Article

Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

Insert

Early growth response 3 (Egr3 Mouse)

Use

AAV

Tags

EYFP

Promoter

EF-1-alpha

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18

Plasmid

#172317

Purpose

CRISPR dCas9 directly fused to a catalytic inactive version of bacterial DNA methyltransferase MQ1 to target to the FWA locus with three guide RNAs

Depositor

Steven Jacobsen

Article

Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(C141S,S317A)_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pHYRSF236

Plasmid

#46377

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

NpuDnaE intein delta variant, inactive

Tags

H6-Smt3

Expression

Bacterial

Mutation

C1A, C+1A, deleted T76, V97, D98, L100 from NpuDn…

Promoter

T7

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-PiTq

Plasmid

#248769

Purpose

Mammalian expression vector for PiTq

Depositor

Gary Yellen

Article

Rosen et al Proc Natl Acad Sci U S A. 2026 Mar 31;12

Insert

Pi-Tq

Use

AAV

Expression

Mammalian

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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