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LV_EF1a_CDK1(T14A,Y15F)-P2A-Hygro_Barcode

Plasmid

#170220

Purpose

Barcoded lentiviral vector to express CDK1 (T14A, Y15F) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK1 (CDK1 Human)

Use

Lentiviral

Mutation

T14A, Y15F

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC6-GPT2-3xFLAG

Plasmid

#163449

Purpose

lentiviral overexpression vector for human GPT2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

GPT2 (GPT2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

Ubc

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDR FLIP43 GPS1-NR

Plasmid

#101085

Purpose

GA biosensor expression plasmid

Depositor

Wolf Frommer, Alexander Jones

Article

Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

Insert

GPS1-NR

Tags

6HIS and cMyc

Expression

Yeast

Mutation

AtGAI E51K E54R, AtGID1C S114A F115A

Promoter

pPMA1

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Glt1a

Plasmid

#98705

Purpose

mammalian expression of Glt1a

Depositor

Paul Rosenberg

Article

Chen et al J Neurosci. 2002 Mar 15;22(6):2142-52.

Insert

Glt1a (Slc1a2 Rat)

Expression

Mammalian

Mutation

I288V (please see depositor comments below)

Promoter

CMV

Available Since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBPJ_WT

Plasmid

#82158

Purpose

Gateway Donor vector containing RBPJ , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RBPJ (RBPJ Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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JMC034-H11

Plasmid

#58755

Purpose

bacterial expression of PDB 4NW2

Depositor

Cheryl Arrowsmith

Insert

PDB:4NW2 (CHD1 Human)

Tags

His

Expression

Bacterial

Mutation

Bacterial expression for structure determination;…

Promoter

T7

Available Since

Aug. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
JMC02A-C10

Plasmid

#58754

Purpose

bacterial expression of PDB 4O61

Depositor

Cheryl Arrowsmith

Insert

ALKBH5 (ALKBH5 Human)

Tags

His

Expression

Bacterial

Mutation

Bacterial expression for structure determination;…

Promoter

T7

Available Since

Aug. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pIOM14

Plasmid

#20868

Depositor

Carmen Beuzón

Article

Ortiz-Martin et al J Microbiol Methods. 2006 Dec . 67(3):39

Insert

aphA::aacC1

Expression

Bacterial

Available Since

July 15, 2009

Availability

Academic Institutions and Nonprofits only
p064 pCS mTRPS1 P1

Plasmid

#11035

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 P1 (Trps1 Mouse)

Use

Xenopus, mammalian, avian, and zebrafish

Mutation

Truncation at aa338 to mimic C338X mutation found…

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only
CMV-SNAP-hCB2_IRES_EGFP

Plasmid

#223506

Purpose

Expression of CB2 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection marker

Depositor

James Frank

Article

Kosar et al J Am Chem Soc. 2023 Jul 19;145(28):15094

Insert

SNAP-tag-CNR2 and EGFP (CNR2 Synthetic, Human)

Tags

SNAP-tag

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode

Plasmid

#170240

Purpose

Barcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Mutation

S218D, S222D

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1aGFP2AKAT7(E508Q)-W

Plasmid

#159293

Purpose

Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, E508Q mutant) driven by human EF1a promoter

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

KAT7 E508Q

Use

Lentiviral

Expression

Mammalian

Mutation

CRISPR sites mutated (gRNA ID. 5 and A10) and E50…

Promoter

human EF1a promoter

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA

Plasmid

#64973

Purpose

Expresses the wild type of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

T7 promoter

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pHJ41

Plasmid

#48360

Purpose

Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Blasticidin/Gancyclovir selectable

Depositor

George Cross

Article

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-BSD-Ty1-TK-loxP-3' ALD UTR

Use

Cre/Lox; Knockout in t. brucei

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pMM2-rPMCA3b

Plasmid

#47763

Purpose

mammalian expression of rat PMCA3b

Depositor

John Penniston, Emanuel Strehler

Article

Enyedi et al J Biol Chem. 1997 Oct 31;272(44):27525-8

Insert

PMCA3b (Atp2b3 Rat)

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only
pPZP FLIPmal-25u

Plasmid

#14476

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPmal

Use

Plant nanosensor

Tags

ECFP and EYFP

Expression

Plant

Mutation

W230A

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pSP64R1 SHP2 D61A

Plasmid

#8326

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Use

Xenopus expression

Mutation

D61A (activated mutant)

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn1-eYFP (AAV1)

Viral Prep

#117382-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV-hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified pAAV-hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV preparations are suitable purity for injection into animals.

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Promoter

Syn

Tags

eYFP

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only
GB1-A11(2-196)-Strep

Plasmid

#196208

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-Puro_mouse_Adar2_Tv1

Plasmid

#158111

Purpose

expresses mADAR2 shortest transcript, puromycin selection

Depositor

Emanuela Felley-Bosco

Article

Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

Insert

adar2 (Adarb1 Mouse)

Expression

Mammalian

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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