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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,962 results for: pcDNA3.1+

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Showing: 4241 - 4260 of 6962 results

  2. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]

    Plasmid
    #227246
    Purpose
    All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell
    Depositor
    Jae Seong Lee
    Publication
    Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.
    Inserts
    Tet-On 3G transactivator protein
    Enhanced green fluorescent protein
    Use
    Cre/Lox and Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF1α and pTRE3G
    Available since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    227246-plasmid-map-sequence-id-450569

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  3. eGFP-SUN1

    Plasmid
    #213515
    Purpose
    Expresses human eGFP-SUN1 in mammalian cells
    Depositor
    Karim Mekhail
    Publication
    Shokrollahi et al Nat Struct Mol Biol. 2024 Sep;31(9):1319
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SUN1 (SUN1 Human)
    Expression
    Mammalian
    Mutation
    SUN1 has a silent mutation on its gene (nucleotid…
    Promoter
    CMV
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    213515-plasmid-map-sequence-id-422354

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  4. pc3.1-CAGGS-ASAP3-mCyRFP3

    Plasmid
    #193323
    Purpose
    A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3
    Depositor
    Michael Lin
    Publication
    Kim et al Sci Rep. 2022 Mar 7;12(1):3678. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ASAP3-R3
    Expression
    Mammalian
    Promoter
    caggs
    Available since
    Oct. 13, 2023
    Availability
    Academic Institutions and Nonprofits only

  8. pCMV-ACBEmax

    Plasmid
    #139106
    Purpose
    Mammalian ACBEmax expression vector (pSI-632)
    Depositor
    Nozomu Yachie
    Publication
    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    139106-plasmid-map-sequence-id-273273

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  9. p4B5-EGFP

    Plasmid
    #140324
    Purpose
    Detection of Dengue virus infection in live cells
    Depositor
    Alan Rothman
    Publication
    Medin et al J Virol Methods. 2015 Jan;211:55-62. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DENV2-NGC NS4b5(10 a.a.)-NLS-eGFP
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    2 conservative n.t. differences near 5' end …
    Promoter
    CMV
    Available since
    May 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    140324-plasmid-map-sequence-id-296784

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  14. pTE4889

    Plasmid
    #88905
    Purpose
    Expresses FnCpf1 crRNA and inactive/dead, humanized FnCpf1 nuclease
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Fn crRNA
    hFnCpf1(D917A)
    Use
    CRISPR
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Promoter
    CMV and human U6
    Available since
    April 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    88905-plasmid-map-sequence-id-176331

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  16. VBS mutant of alpha catenin sensor

    Plasmid
    #71710
    Purpose
    This form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.
    Depositor
    Deborah Leckband
    Publication
    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    alpha-catenin ΔVBS FRET Sensor
    Tags
    ECFP and YPET
    Expression
    Mammalian
    Mutation
    Deleted amino acid residues 316-405 in α -catenin…
    Promoter
    CMV
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71710-plasmid-map-sequence-id-142366

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  18. pcDNA3-NGFP-VMAn-FKBP

    Plasmid
    #70225
    Purpose
    Expresses N-terminal portion of GFP along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Publication
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eGFP
    Tags
    FK506- and rapamycin-binding protein (FKBP), Link…
    Expression
    Mammalian
    Mutation
    Changed Glu 125 to Ile, Deleted amino acids 129-2…
    Promoter
    CMV
    Available since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  19. pcDNA3-FRB-VMAc-CGFP

    Plasmid
    #70226
    Purpose
    Expresses C-terminal portion of GFP along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Publication
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eGFP
    Tags
    FKBP-rapamycin binding (FRB) domain of the mammal…
    Expression
    Mammalian
    Mutation
    Changed Ile 129 to Cys, Deleted amino acids 1-128
    Promoter
    CMV
    Available since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
Showing: 4241 - 4260 of 6962 results