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pQE30-hPolB

Plasmid

#70761

Purpose

bacterial expression of human DNA Polymerase beta (POLB)

Depositor

Primo Schaer

Article

Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

Insert

POLB (POLB Human)

Use

Tags

6x His

Expression

Bacterial

Mutation

R333L

Promoter

T5/lac operon

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pQE30-hPolB

Plasmid

#70761

Purpose

bacterial expression of human DNA Polymerase beta (POLB)

Depositor

Primo Schaer

Article

Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

Insert

POLB (POLB Human)

Use

Tags

6x His

Expression

Bacterial

Mutation

R333L

Promoter

T5/lac operon

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pET His6 NusA TEV LIC cloning vector (2N-T)

Plasmid

#29709

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6-NusA-TEV

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pET His6 NusA TEV LIC cloning vector (2N-T)

Plasmid

#29709

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6-NusA-TEV

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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TFORF2908

Plasmid

#143663

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NFIC (NFIC Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2908

Plasmid

#143663

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NFIC (NFIC Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-T/O-Ds1-mCherry

Plasmid

#112855

Purpose

Dox inducible expression in mammalian cells of Ds1 protein fused to mCherry fluorescent protein

Depositor

David Sprinzak

Article

Loza et al Elife. 2017 Aug 18;6. pii: 24820. doi: 1

Insert

DCHS1 (DCHS1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

doxycycline inducible promoter

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-T/O-Ds1-mCherry

Plasmid

#112855

Purpose

Dox inducible expression in mammalian cells of Ds1 protein fused to mCherry fluorescent protein

Depositor

David Sprinzak

Article

Loza et al Elife. 2017 Aug 18;6. pii: 24820. doi: 1

Insert

DCHS1 (DCHS1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

doxycycline inducible promoter

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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TRPML1-G-GECO1.2-ERES

Plasmid

#207144

Purpose

Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signal

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

Mcoln1 (MCOLN1 Human)

Use

Tags

ER-Export Signal (ERES) and G-GECO1.2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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TRPML1-G-GECO1.2-ERES

Plasmid

#207144

Purpose

Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signal

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

Mcoln1 (MCOLN1 Human)

Use

Tags

ER-Export Signal (ERES) and G-GECO1.2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pDB5319

Plasmid

#204829

Purpose

An integrating plasmid containing both the inducible enotetSW1 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

Depositor

Li-Lin Du

Article

Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

Inserts

tetR
mECitrine

Use

Tags

Expression

Yeast

Mutation

Promoter

CMV promoter and enotetSW1 promoter

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pDB5319

Plasmid

#204829

Purpose

An integrating plasmid containing both the inducible enotetSW1 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

Depositor

Li-Lin Du

Article

Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

Inserts

tetR
mECitrine

Use

Tags

Expression

Yeast

Mutation

Promoter

CMV promoter and enotetSW1 promoter

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMIG-Aalpha WT

Plasmid

#10884

Depositor

William Hahn

Article

Chen et al Cancer Res. 2005 Sep 15. 65(18):8183-92.

Insert

PP2A Aalpha (PPP2R1A Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pMIG-Aalpha WT

Plasmid

#10884

Depositor

William Hahn

Article

Chen et al Cancer Res. 2005 Sep 15. 65(18):8183-92.

Insert

PP2A Aalpha (PPP2R1A Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_EBFP_Donor

Plasmid

#178089

Purpose

HDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EBFP (T65S/F145Y)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human PGK1

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_EBFP_Donor

Plasmid

#178089

Purpose

HDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EBFP (T65S/F145Y)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human PGK1

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET His6 Mocr TEV LIC cloning vector (1O)

Plasmid

#29658

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

6xHis, Mocr, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pET His6 Mocr TEV LIC cloning vector (1O)

Plasmid

#29658

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

6xHis, Mocr, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-MD2-HA

Plasmid

#133065

Purpose

Retroviral expression of mouse MD2 tagged with HA

Depositor

Jonathan Kagan

Article

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

MD2 (Ly96 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-MD2-HA

Plasmid

#133065

Purpose

Retroviral expression of mouse MD2 tagged with HA

Depositor

Jonathan Kagan

Article

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

MD2 (Ly96 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge