We narrowed to 7,643 results for: RAP

Showing: 4261 - 4280 of 7643 results

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pCR2.1 GFR alpha 2

Plasmid

#119899

Purpose

Mouse GFR alpha 2 In situ hybridization probe

Depositor

David Ginty, Wenqin Luo

Article

Luo et al Neuron. 2009 Dec 24;64(6):841-56. doi: 1

Insert

GDNF family receptor alpha-2 (Gfra2 Mouse)

Use

In situ hybridization probe

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSCIP-mB2M

Plasmid

#154091

Purpose

Self-Cutting and Integrating CRISPR backbone targeting murine B2M

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Insert

sgRNA and BAIT targeting murine B2M locus

Use

CRISPR

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-LexAOx4-Pmin-Fluc-PGK-Rluc-P2A-mCherry

Plasmid

#192634

Purpose

Lentiviral vector for inducible Fluc expression with 4x LexA operator (LexAO) and constitutive Rluc and mCherry.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

firefly luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal promoter

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pMK280 (mCherry2-NeoR)

Plasmid

#72796

Purpose

C-terminal tagging with mCherry2 using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mCherry2-Bsr

Use

CRISPR

Tags

mCherry2

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-2

Plasmid

#172768

Purpose

Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-2

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSBbi_RP_MicroID2-FLAG-NES

Plasmid

#188525

Purpose

Encodes the biotin proximity labeling enzyme MicroID2 with a nuclear export sequence (NES) for expression and stable integration into mammalian cells

Depositor

James Londino

Article

Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

Insert

MicroID2

Use

Transposon mediated gene integration

Tags

FLAG

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTS95 pHAGE2 CMVtetO2 GFP-22xGS-TEV-MCS

Plasmid

#199348

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-TEV-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP-TEV

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS97 pHAGE2 CMVtetO2 GFP-22xGS-SUMOstar-MCS

Plasmid

#199350

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOstar-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP-SUMOstar

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3C WT

Plasmid

#123078

Purpose

Gateway entry clone encoding human MAP1LC3C wild-type

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)

Use

Gateway entry vector / entry clone

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMK279 (mClover-Bsr)

Plasmid

#72795

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mClover-Bsr

Use

CRISPR

Tags

mClover

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pHD157

Plasmid

#84033

Purpose

Vector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the fabp10 promoter and Venus under the crystalin promoter in zebrafish

Depositor

Daniel Hesselson, Didier Stainier

Article

Ni et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Inserts

Cre
Venus

Use

Cre/Lox; Zebrafish transgenesis

Tags

mCherry

Promoter

Crystalin and fabp10

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-JEDI-1P-WPRE

Plasmid

#202615

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P

Use

AAV

Expression

Mammalian

Promoter

CaMKIIa

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMP71-mGFP-LLO-mPMEL

Plasmid

#174608

Purpose

expresses palmitoylated GFP with a C terminal fusion of the LLO190 epitope and the murine hPMEL epitope

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

membrane GFP fused to LLO and mouse PMEL antigen

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pRDA_836

Plasmid

#216088

Purpose

Cas9 [Sp] knockout targeting CD55, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD55 guide

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pOZ-N-FH Abraxas

Plasmid

#27495

Depositor

Roger Greenberg

Article

Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

Insert

Abraxas (FAM175A Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
pBS hBAF53a

Plasmid

#17879

Depositor

Jerry Crabtree

Article

Zhao et al Cell. 1998 Nov 25. 95(5):625-36.

Insert

BAF53a (ACTL6A Human)

Expression

Bacterial

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pLVX Flag RagC WT

Plasmid

#112756

Purpose

express Flag-RagC

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (RRAGC Human)

Use

Lentiviral

Tags

Flag

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-30

Plasmid

#172754

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-30

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_(GGS)6_SNIPR

Plasmid

#188344

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)6, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_(GGS)6_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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J23100_GB

Plasmid

#65983

Purpose

MoClo Basic Part: Constitutive promoter - Anderson series - high strength [G:J23100:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Constitutive promoter

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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