We narrowed to 62,258 results for: cloning

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pET28a-MH6-Bsu

Plasmid

#163911

Purpose

Expresses Bsu large fragment for affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

Bsu polymerase

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C9orf72

Plasmid

#83437

Purpose

Expresses GFP-C9orf72 in mammalian cells

Depositor

Shawn Ferguson

Article

Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

Insert

C9orf72 (C9orf72 Human)

Tags

GFP

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pUAST-pHuji-GINKO2

Plasmid

#177117

Purpose

Genetically encoded green fluorescent potassium ion indicator GINKO2 and red fluorescent pH indicator pHuji fusion

Depositor

Robert Campbell

Article

Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

Insert

pHuji-GINKO2

Expression

Insect

Promoter

hsp70 promoter

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

Plasmid

#175574

Purpose

Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-SaPE2

Plasmid

#174817

Purpose

Mammalian expression of SaCas9 prime editor 2

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

SaPE2

Tags

SV40 bpNLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST14-Cys-DogCatcher

Plasmid

#171929

Purpose

Express Cys-DogCatcher protein in bacterial cytoplasm. The cysteine enables convenient coupling to maleimide-dye or thiol-reactive resin.

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

Cys-DogCatcher

Tags

His6 and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pSF463

Plasmid

#167671

Purpose

Intermediate vector for two gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-SEP-GluA1 TKIT

Plasmid

#169441

Purpose

Expression of 2 guides + donor DNA

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

AAV and CRISPR

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

Plasmid

#168290

Purpose

CRISPR-activator Suntag-P65-HSF1 (SPH)

Depositor

Hui Yang

Article

Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

Insert

dCas9, Suntag-P65-HSF1

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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Nrxn3b-Nav1.6-MKII-f-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16

Plasmid

#163031

Purpose

FLiCRE TF with fast kinetics for neuronal expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

Nrxn3b-MKII-f-hLOV1-TEVcs-tTA

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAX

Plasmid

#164611

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in mammalian cells

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Expression

Mammalian

Mutation

S383T

Promoter

CMV

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDisplay-iSeroSnFR

Plasmid

#128484

Purpose

Fluorescent reporter for serotonin (mammalian expression, membrane localization tag)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Insert

iSeroSnFR

Tags

Ig-kappa leader, Myc, PDGFR + enhanced ER export,…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3 CMV sTurboID (C)-Arrestin-GFP

Plasmid

#153010

Purpose

Expresses split-TurboID C-terminal fragment fused to arrestin

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID (C)(G74)-Arrestin

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_FLAG-Mma PylRS

Plasmid

#140009

Purpose

Plasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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QPM-sgR (pTaU3)

Plasmid

#140448

Purpose

For sgRNA expression in monocotyledons protoplasts

Depositor

Caixia Gao

Article

Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

Insert

TaU3-sgRNA

Use

CRISPR

Expression

Plant

Promoter

TaU3

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pET16b-PCNA

Plasmid

#134898

Purpose

Bacterial expression and purification of human PCNA

Depositor

Andrew Deans

Article

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Insert

PCNA (PCNA Human)

Tags

untagged

Expression

Bacterial

Mutation

fully synthetic, codon optimized for bacterial ex…

Promoter

T7

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-Q331K

Plasmid

#133761

Purpose

Lentiviral expression of human TDP-43 Q331K disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed glutamine 331 to lysine.

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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R4pGWB601_UBQ10p-miniTurbo-NES-YFP

Plasmid

#127369

Purpose

Binary vector for expressing cytosolic miniTurbo-YFP under the UBQ10 promoter in plants

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Tags

GS linker, NES, V5, and YFP

Expression

Plant

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

Arabidopsis UBQ10 promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Lrp6

Plasmid

#123595

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

low density lipoprotein receptor-related protein 6 (Lrp6 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGT-S1

Plasmid

#122582

Purpose

MAGIC donor plasmid (RSF1010 oriR with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

beta-lactamase/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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