We narrowed to 5,308 results for: pcDNA3.1+

1. HiBiT-FZD10

   Plasmid

   #195848

   Purpose

   Expresses human FZD10 with an N-terminal HiBiT

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD10 (FZD10 Human)
   

   Use

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. canxTM-mEmerald (cytosolic)

   Plasmid

   #186962

   Purpose

   Expresses mEmerald connected with the C-terminus of Calnexin transmembrane domain.

   Depositor

   Ke Xu

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   CANX (CANX Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Only express calnexin (482-end) and add ER signal…

   Promoter

   CMV Promoter

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDest-303

   Plasmid

   #159678

   Purpose

   Gateway destination vector for mammalian protein expression

   Depositor

   Dominic Esposito

   Article

   Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

   Plasmid

   #137830

   Purpose

   MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factor

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Mutated Leucine 71 to Lysine in CTEVp fragment

   Promoter

   CMV

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Diacylglycerol Kinase Zeta

   Plasmid

   #85454

   Purpose

   expression of Diacylglycerol Kinase Zeta

   Depositor

   Stephen Gee

   Article

   Hogan et al J Biol Chem. 2001 Jul 13;276(28):26526-3

   Insert

   Diacylglycerol Kinase Zeta (DGKZ Human)
   

   Use

   Tags

   3x HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FLAG-hIes6

   Plasmid

   #15356

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

   Insert

   hIes6 (INO80C Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

7. CoV2-N-R203K

   Plasmid

   #177951

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203K). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (R203K) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R203K

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HiBiT-FZD5

   Plasmid

   #195843

   Purpose

   Expresses human FZD5 with an N-terminal HiBiT

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD5 (FZD5 Human)
   

   Use

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HiBiT-FZD3

   Plasmid

   #195841

   Purpose

   Expresses human FZD3 with an N-terminal HiBiT; contains I292V and V495I

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD3 (FZD3 Human)
   

   Use

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   I292V and V495I

   Promoter

   CMV

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-D2s-L-V1

    Plasmid

    #19967

    Depositor

    Jonathan Javitch

    Article

    Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

    Insert

    D2 short variant-N terminal Venus (DRD2 Human, Aequorea Victoria)
    

    Use

    Tags

    Flag and N terminal Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

11. FLYLQ

    Plasmid

    #80421

    Purpose

    expresses cDNA for human CELF1 (CUGBP1) Flag tagged

    Depositor

    Thomas Cooper

    Article

    Kalsotra et al Proc Natl Acad Sci U S A. 2008 Dec 23;10

    Insert

    human CELF1 cDNA (CELF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ATG9A-2STrep

    Plasmid

    #210359

    Purpose

    Expresses ATG9A fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    ATG9A (ATG9A Human)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CoV2-N-G204R

    Plasmid

    #177953

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (G204R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (G204R) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G204R

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CIB-VP16 (B1014)

    Plasmid

    #92036

    Purpose

    Expresses CIB1 (full length, with endogenous NLS) fused to VP16 activation domain.

    Depositor

    Chandra Tucker

    Article

    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

    Insert

    CIB1 (CIB1 Mustard Weed)
    

    Use

    Tags

    Fusion of plant CIB1 (intact NLS) with VP16 activ…

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FLAG HDAC5 S259A/S498A

    Plasmid

    #32216

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    HDAC5 (HDAC5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser259Ala, Ser498Ala

    Promoter

    CMV

    Available since

    Oct. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-LYRa11-RBD-8h

    Plasmid

    #161822

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate LYRa11

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Use

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T324-D522)

    Promoter

    CMV

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Nuc-mTurq2-Apollo-NADP+

    Plasmid

    #193302

    Purpose

    Expresses mTurq2-Apollo-NADP+ sensor localized to the nucleus

    Depositor

    Jonathan Rocheleau

    Article

    Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

    Insert

    G6PD (G6PD Human)
    

    Use

    Tags

    SV40 NLS and mTurqoise2

    Expression

    Mammalian

    Mutation

    S40A, R72Q, H201N, K205T

    Promoter

    T7

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CaMKIV-DCTK75E

    Plasmid

    #126423

    Purpose

    A kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

    Depositor

    Douglas Black

    Article

    Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

    Insert

    calcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed lysine 75 to glutamic acid

    Promoter

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NSP3C-Flag

    Plasmid

    #210349

    Purpose

    Expresses the C terminal of SARS-CoV-2 NSP3 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    C terminal of SARS-CoV-2 NSP3 (ORF1ab )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAB07 (CK2beta)

    Plasmid

    #27091

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2beta (CSNK2B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2012

    Availability

    Academic Institutions and Nonprofits only