We narrowed to 13,845 results for: Cas9

1. pTX200

   Plasmid

   #89264

   Purpose

   Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA01

   Depositor

   Daniel Voytas

   Article

   Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

   Insert

   OsYSA-gRNA01
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Plant

   Mutation

   Promoter

   Maize Ubiquitin1 promoter

   Available since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTX199

   Plasmid

   #89263

   Purpose

   Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA02

   Depositor

   Daniel Voytas

   Article

   Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

   Insert

   OsPDS-gRNA02
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Plant

   Mutation

   Promoter

   Maize Ubiquitin1 promoter

   Available since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSB700 Cerulean-Weissman scaffold

   Plasmid

   #79377

   Purpose

   Cas9 gRNA variant to recruit ms2 proteins

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSB700 Cerulean-Zhang2.0

   Plasmid

   #79378

   Purpose

   Cas9 gRNA variant to recruit ms2 proteins

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   zhang 2.0
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-dSa-VPR

   Plasmid

   #99651

   Purpose

   Expresses tripartite VP64-p65-RTA activator fused to C term. of dead Sa Cas9

   Depositor

   George Church

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VP64-p65-RTA activator

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6(BsmBI)-EF1a-BFP-BSD

   Plasmid

   #117136

   Purpose

   Empty vector encoding BFP-BSD

   Depositor

   Kosuke Yusa

   Insert

   BSD2A
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   modified BFP

   Promoter

   human U6 promoter, human EF1a promoter

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lentiviral sgRNA human CD19

   Plasmid

   #155289

   Purpose

   Lentiviral expression of sgRNA against human CD19

   Depositor

   Martin Carroll

   Insert

   CD19 (CD19 Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT3TS-zSpG

   Plasmid

   #179316

   Purpose

   In vitro transcription of SpG-Cas9 mRNA zebrafish codon-optimized from T3 promoter

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

   Insert

   Zebrafish codon-optimized Cas9 variant SpG
   

   Use

   CRISPR; In vitro transcription by t3 promoter

   Tags

   SV40-NLS

   Expression

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT3TS-zSpRY

   Plasmid

   #179317

   Purpose

   In vitro transcription of SpRY-Cas9 mRNA zebrafish codon-optimized from T3 promoter

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

   Insert

   Zebrafish codon-optimized Cas9 variant SpRY
   

   Use

   CRISPR; In vitro transcription by t3 promoter

   Tags

   SV40-NLS

   Expression

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUDP002

    Plasmid

    #103872

    Purpose

    Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Inserts

    hph
    Spcas9 D147Y P411T
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Yeast

    Mutation

    D147Y P411T

    Promoter

    Arxula adeninivorans TEF1 and Ashbya gossypii (Er…

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUDP123

    Plasmid

    #107269

    Purpose

    pUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. TLCV2

    Plasmid

    #87360

    Purpose

    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Insert

    Cas9-2A-eGFP
    

    Use

    CRISPR and Lentiviral; Doxycycline inducible; egf…

    Tags

    Cas9-T2A-eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. YE1-BE4max

    Plasmid

    #138155

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(YE1)-nSpCas9-UGI-UGI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 W90Y + R126E; within Cas9 D10A

    Promoter

    CMV

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. AALN-BE4max

    Plasmid

    #138161

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(AALN)-nSpCas9-UGI-UGI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 R33A + K34A + H122L + D124N; with…

    Promoter

    CMV

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pST_116_LVL2 cam

    Plasmid

    #179332

    Purpose

    NT-CRISPR plasmid for a single gRNA.

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-multi-Guide

    Plasmid

    #85401

    Purpose

    Lentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neo

    Depositor

    Qin Yan

    Article

    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. AIO-Puro

    Plasmid

    #74630

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Puromycin resistant marker

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    May 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pS648∙CsR

    Plasmid

    #197860

    Purpose

    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    gRNA scaffold - Gm resistance cassette - incP oriT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PEM7

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCB578

    Plasmid

    #141095

    Purpose

    E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array

    Depositor

    Chase Beisel

    Article

    Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

    Inserts

    Cas9 and tracrRNA
    repeat-spacer-repeat array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-multi-CRISPR

    Plasmid

    #85402

    Purpose

    Lentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expression

    Depositor

    Qin Yan

    Article

    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge