We narrowed to 19,782 results for: IRE

1. pET30Xa-SBL1(160E)

   Plasmid

   #66846

   Purpose

   Expresses soybean lipoxygenase-1 S160E mutant with N-term Histag

   Depositor

   Betty Gaffney

   Article

   Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

   Insert

   soybean lipoxygenase-1 (LOC547694, NEWENTRY Glycine max)
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   S160E

   Promoter

   T7lac

   Available since

   Sept. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET30Xa-SBL1(160S,NoCys)

   Plasmid

   #66877

   Purpose

   Expresses a cysteine-free version of WTsoybean lipoxygenase-1 160S (4Cys to Ser) with N-term Histag

   Depositor

   Betty Gaffney

   Article

   Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

   Insert

   soybean lipoxygenase-1
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   four (all) cysteines mutated to serine

   Promoter

   T7lac

   Available since

   Aug. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGFP-N1-hRIP1-FL I541P

   Plasmid

   #61381

   Purpose

   Expresses Human full length RIPK1 with I541P mutation in the mammalian cells

   Depositor

   Francis Chan

   Article

   Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

   Insert

   Full length human RIP1with I541P mutation (RIPK1 Human)
   

   Use

   Tags

   EGFP and HA tag

   Expression

   Mammalian

   Mutation

   changed I541 to P

   Promoter

   Available since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pUASp-Flag-Myc-Qin-Tudor-attB

   Plasmid

   #44210

   Depositor

   Phillip Zamore

   Article

   Zhang et al Mol Cell. 2011 Nov 18;44(4):572-84. doi:

   Insert

   Qin-Tudor-domain (qin Fly)
   

   Use

   Fly transformation

   Tags

   Flag and Myc

   Expression

   Mutation

   lacks E3 domain on N-term (del aa1-274)

   Promoter

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

5. DIRAS2

   Plasmid

   #55670

   Purpose

   expression of mouse DIRAS2

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   DIRAS2 (Diras2 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

6. mutpRL-TK 6xUTR

   Plasmid

   #40761

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   6 copies of the CXCR4 small RNA target site
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

7. mutpRL-TK 2xUTR

   Plasmid

   #40757

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   2 copies of the CXCR4 small RNA target site
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

8. mutpRL-TK 4xUTR

   Plasmid

   #40759

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   4 copies of the CXCR4 small RNA target site
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

9. mutpRL-TK 5xUTR

   Plasmid

   #40760

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   5 copies of the CXCR4 small RNA target site
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

10. mutpRL-TK 1xUTR

    Plasmid

    #40756

    Purpose

    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

    Depositor

    Phillip Zamore

    Article

    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

    Insert

    CXCR4 small RNA target site
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    mutated from TAG to CTC at nt 387–389 of the Reni…

    Promoter

    TK

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

11. TRS6E1b-luc(-732/-721) mutant1

    Plasmid

    #45384

    Depositor

    Thomas Gelehrter

    Article

    Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

    Insert

    6 copies of hPAI1 promoter -732/-721 mutated at -732/-731
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    mutated -732/-731 from ag to tc

    Promoter

    Available since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

12. Abr-pLEF

    Plasmid

    #38157

    Depositor

    Nora Heisterkamp

    Article

    Kweon et al J Biol Chem. 2008 Feb 8;283(6):3023-30.

    Insert

    ABR (ABR Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Aug. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA4-rB90

    Plasmid

    #18959

    Depositor

    Mariano Viapiano

    Article

    Viapiano et al J Neurooncol. 2008 Jul . 88(3):261-72.

    Insert

    Brevican C-terminus (Bcan Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Rat brevican C-terminus: Signal peptide aa 1-22 …

    Promoter

    Available since

    Dec. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pGEX-2TK-WW-EF1+EF2

    Plasmid

    #18080

    Depositor

    Marius Sudol

    Article

    Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

    Insert

    Dystrophin (DMD Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1-Cerulean-Epac2(R53A)-Venus

    Plasmid

    #138197

    Purpose

    FRET-based Epac2 activation sensor with R53A mutation in Epac2 coding sequence.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2(R53A)-Venus (Rapgef4 Synthetic, Mouse)
    

    Use

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Mutation

    Contains arginine-to-alanine substitution at resi…

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-Cerulean-Epac2(R447)-cpV[E172]

    Plasmid

    #138198

    Purpose

    FRET-based Epac2 activation sensor with R447A mutation in Epac2 coding sequence.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2(R447A)-cpVenus[E172] (Rapgef4 Synthetic, Mouse)
    

    Use

    Tags

    Cerulean and cpV[E172]

    Expression

    Mammalian

    Mutation

    Contains arginine-to-alanine substitution at resi…

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-Cerulean-Epac2(R466A)-Venus

    Plasmid

    #138199

    Purpose

    FRET-based Epac2 activation sensor with R466A mutation in Epac2 coding sequence.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2(R466A)-Venus (Rapgef4 Synthetic, Mouse)
    

    Use

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Mutation

    Contains arginine-to-alanine substitution at resi…

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-Cerulean-Epac2(L426W)-Venus

    Plasmid

    #138200

    Purpose

    FRET-based Epac2 activation sensor with L426W mutation in Epac2 coding sequence.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2(L426W)-Venus (Rapgef4 Synthetic, Mouse)
    

    Use

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Mutation

    Contains leucine-to-tryptophan substitution at re…

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYL192 (TRV1)

    Plasmid

    #148968

    Purpose

    Tobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

    Insert

    TRV RNA1
    

    Use

    Plant vector with trv rna1; this vector used with…

    Tags

    Expression

    Mutation

    P697S mutation in putative replicase. S239G and S…

    Promoter

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TFORF3549

    Plasmid

    #145025

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEGFP-NUP188

    Plasmid

    #159893

    Purpose

    Expresses NUP188 in mammalian cells

    Depositor

    Kozo Tanaka

    Article

    Itoh et al Cancer Sci. 2013 Jul;104(7):871-9. doi:

    Insert

    NUP188 (NUP188 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    To avoid NotI site, first three amino acids are d…

    Promoter

    CMV

    Available since

    Oct. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TFORF1652

    Plasmid

    #143532

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SPI1 (SPI1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TFORF0518

    Plasmid

    #141628

    Purpose

    Lentiviral vector for overexpressing the PAX5 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PAX5 (PAX5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GFP-ZMYND8

    Plasmid

    #65401

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    ZMYND8 (ZMYND8 Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TFORF3376

    Plasmid

    #144852

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TOX (TOX Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-mBATF

    Plasmid

    #34575

    Depositor

    Peter Murray

    Article

    Smith et al J Biol Chem. 2011 Jul 1;286(26):23582-90

    Insert

    BATF (Batf Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA3.1-TRKB-V5/HIS

    Plasmid

    #205614

    Purpose

    Expression of human TRKB receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    TRKB receptor tyrosine kinase (NTRK2 Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TFORF0509

    Plasmid

    #143036

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TSHZ2 (TSHZ2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TFORF3399

    Plasmid

    #144875

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    POU2F2 (POU2F2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3 HA E2F1

    Plasmid

    #236431

    Purpose

    transient overexpression of E2F1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F1 (E2F1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TFORF0951

    Plasmid

    #144266

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV.hSyn.iGluSnFr.WPRE.SV40

    Plasmid

    #98929

    Purpose

    AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    iGluSnFr
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. TFORF3550

    Plasmid

    #145026

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGL3Luc-5XNF-kappaB

    Plasmid

    #185695

    Purpose

    Reporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').

    Depositor

    Esther López-Bayghen

    Article

    Hernandez-Melchor et al ASN Neuro. 2022 Jan-Dec;14:1759091422111

    Insert

    NF-kappaB (NFKB1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    NF-kappaB binding site

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEGPN-TMPRSS2

    Plasmid

    #187484

    Purpose

    Expression vector - TMPRSS2

    Depositor

    Lewis Cantley

    Article

    Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

    Insert

    TMPRSS2 (TMPRSS2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. TFORF2093

    Plasmid

    #143337

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZFP14 (ZFP14 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pPB-CAG-hCas3

    Plasmid

    #134920

    Purpose

    Components for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cas3 with bpNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1-BCR-ABL-p190-FLAG

    Plasmid

    #205620

    Purpose

    Expression of BCR-ABL oncogene in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

    Insert

    BCR-ABL oncogene, p190 isoform b3a2
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T117M- Please see depositor comments

    Promoter

    CMV

    Available since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLVX-XBP1 mNeonGreen NLS

    Plasmid

    #115968

    Purpose

    ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen

    Depositor

    David Andrews

    Article

    Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

    Insert

    X-box binding protein 1 (XBP1 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    HA tag, c-myc NLS, and mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3/Myc-DNMT1

    Plasmid

    #36939

    Purpose

    Mammalian expression of human DNMT1 with myc tag

    Depositor

    Arthur Riggs

    Article

    Li et al J Biol Chem. 2006 Jul 14;281(28):19489-5

    Insert

    DNMT1 (DNMT1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. GFP-EEA1 wt

    Plasmid

    #42307

    Depositor

    Silvia Corvera

    Article

    Lawe et al J Biol Chem. 2000 Feb 4;275(5):3699-705.

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. ABE8.20-m

    Plasmid

    #136300

    Purpose

    expresses ABE8.20-m in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.20-m
    

    Use

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. 7M8

    Plasmid

    #64839

    Purpose

    This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.

    Depositor

    David Schaffer, John Flannery

    Article

    Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra

    Insert

    7M8 cap
    

    Use

    AAV

    Tags

    Expression

    Mutation

    7mer insertion in the AAV2 cap

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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44. pOTTC407 - pAAV EF1a eGFP

    Plasmid

    #60058

    Purpose

    An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

    Insert

    enhanced Green Fluorescent Protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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45. ABE8.8-m

    Plasmid

    #136294

    Purpose

    expresses ABE8.8-m in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.8-m
    

    Use

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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46. pLJC6-3XHA-TMEM192

    Plasmid

    #104434

    Purpose

    Expresses 3XHA-TMEM192 in mammalian cells

    Depositor

    David Sabatini

    Article

    Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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47. TFORF2925

    Plasmid

    #143676

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZFP57 (ZFP57 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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48. phDLX1-N174

    Plasmid

    #60859

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses DLX1 under the EF1a promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    DLX1 (DLX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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49. p7SL-neo-TET

    Plasmid

    #51285

    Purpose

    expresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    7SL RNA (RN7SL2 Human)
    

    Use

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Mutation

    Promoter

    7SL upstream pollII enhancer

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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50. TFORF2029

    Plasmid

    #143566

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SMARCA5 (SMARCA5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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