We narrowed to 62,258 results for: cloning

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pAM1579

Plasmid

#40240

Depositor

Susan Golden

Article

Andersson et al Methods Enzymol. 2000;305:527-42.

Insert

Km Resistance, neutral site II integration platform for Synechococcus

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pLex 983-Micu2

Plasmid

#50372

Purpose

Expression in mammalian cells

Depositor

Vamsi Mootha

Article

Plovanich et al PLoS One. 2013;8(2):e55785. doi: 10.1371

Insert

MICU2 (MICU2 Human)

Use

Lentivirus

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only
Active Wnt7B-V5

Plasmid

#43817

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

human WNT7B (WNT7B Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pγsat

Plasmid

#39238

Depositor

Niall Dillon

Article

Lundgren et al Cell. 2000 Nov 22;103(5):733-43.

Insert

8 copies of the 234bp mouse γ-satellite repeat

Use

Cloning vector

Available Since

Jan. 16, 2013

Availability

Academic Institutions and Nonprofits only
pBad-Xis/Int Reset Flipper

Plasmid

#38207

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

Bxb1-Excisionase
Bxb1 Integrase

Use

Synthetic Biology; Biobrick cloning vector

Promoter

pBAD

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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MSCV-N 16E6

Plasmid

#37875

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

HPV 16E6 (E6 HPV)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

July 25, 2012

Availability

Academic Institutions and Nonprofits only
Flag-HA-LOC220594

Plasmid

#22562

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

LOC220594 (USP32P2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pVS2

Plasmid

#33144

Depositor

Michael Rosbash

Article

Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

Inserts

LacO (256 copies)
GAL-GFP-kd200-GALpA reporter

Expression

Yeast

Mutation

GFP is mutated such that amino acids 64-73 (FSYGV…

Promoter

bidirectional GAL1-GAL10 promoter

Available Since

Dec. 20, 2011

Availability

Academic Institutions and Nonprofits only
pMXs-Sox1

Plasmid

#32931

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Sox1 (Sox1 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only
pENTR-myr-AKT-HA

Plasmid

#31790

Depositor

Xin Chen

Article

Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

Insert

Akt1 (Akt1 Mouse)

Use

Gateway Cloning

Tags

HA and Myr

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-H-RAS V12

Plasmid

#31201

Purpose

Gateway donor vector containing HRAS G12V.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

H-Ras (HRAS Human)

Use

Gateway Cloning

Mutation

G12V constitutively active

Available Since

July 12, 2011

Availability

Academic Institutions and Nonprofits only
pDR-GW FLII12Pglu-700μδ6

Plasmid

#28002

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

FLII12Pglu-700μδ6

Tags

eCFP and eYFP

Expression

Yeast

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pENTR-GFI1B

Plasmid

#16169

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Growth factor independent 1B (GFI1B Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pKM839

Plasmid

#8838

Depositor

David Waugh

Article

Fox et al FEBS Lett. 2003 Feb 27. 537(1-3):53-7.

Type

Empty backbone

Tags

P. furiosus MBP

Expression

Bacterial

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pAAVone-AAV9-CMV-eGFP

Plasmid

#230928

Purpose

pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV9 serotype, utilizing the AAVone single-plasmid AAV production system.

Depositor

Qizhao Wang

Article

Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

Insert

eGFP

Use

AAV

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-miniDq-mCherry-P2A-HA-KORD

Plasmid

#204359

Purpose

AAV vector for coexpression of miniDq and KORD under the control of human synapsin promoter

Depositor

Masao Doi

Article

Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

Insert

miniDq-mCherry-P2A-HA-KORD

Use

AAV

Tags

HA (for KORD) and mCherry (for miniDq)

Mutation

For miniDq, the third intracellular loop (ICL3) o…

Promoter

hSyn

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMS-II-PEST

Plasmid

#204766

Purpose

GEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)

Depositor

Kate Meyer

Article

Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

EGFP-PEST-m6Alinker-DHFR

Mutation

mutated all RACs in EGFP to RGC/RCC

Promoter

CMV

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMN-334

Plasmid

#195468

Purpose

Lentiviral Integratable Inducible synZiFTR ZF10 + Reporter

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

inverted[8X ZF10 BS - ybTATA - mCherry] - pSFFV - ZF10 - NS3 - p65

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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CBE-T1.46

Plasmid

#193284

Purpose

Expression of CBE-T1.46 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CBE-T1.46

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK-rABE-Flag

Plasmid

#191383

Purpose

Doxycycline inducible expression of RNA A-to-I editor rABE

Depositor

Stephen Floor

Article

Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

Insert

rABE-Flag

Use

Lentiviral and Synthetic Biology

Tags

Flag

Expression

Mammalian

Promoter

tet-inducible promoter

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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